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The map label for this gene is gloA

Identifier: 161870729

GI number: 161870729

Start: 1848994

End: 1849410

Strand: Reverse

Name: gloA

Synonym: NMCC_1802

Alternate gene names: 161870729

Gene position: 1849410-1848994 (Counterclockwise)

Preceding gene: 161870730

Following gene: 161870727

Centisome position: 85.88

GC content: 53.24

Gene sequence:

>417_bases
ATGCGCTTACTCCATACTATGCTCCGCGTGGGCAATCTCGAAAAATCCCTCGATTTCTACCAAAACGTTTTGGGTATGAA
ACTGCTCCGCCGAAAAGATTATCCCGAAGGCAGATTTACCCTTGCCTTCGTCGGTTACGGCGACGAAACCGACAGCACGG
TTTTGGAACTGACGCACAACTGGGATACGGAACGATACGACTTGGGCAACGCCTACGGACACATCGCGGTTGAAGTGGAC
GATGCCTACGAAGCCTGCGAACGTGTGAAGCGGCAGGGCGGAAACGTCGTCCGCGAAGCCGGCCCGATGAAACACGGCAC
AACCGTGATAGCCTTCGTCGAAGACCCCGACGGATACAAAATCGAGTTCATTCAAAAGAAAAGCGGTGACGATTCGGTTG
CCTATCAAACTGCCTGA

Upstream 100 bases:

>100_bases
AATCACAAGTCTTTTTCTGGCATTGTCCTGCCTGCAACAAATGGCAGACGTTTACGCCAAACAAAATCGAAGTTTAACCA
CTACCGAGAGGAACACAAAA

Downstream 100 bases:

>100_bases
TACCGCCGCCGCCAATGCCGTCTGAAGCCTTTAGGGGTTTCAGACGGCATTTTGTTGCCGTCGACCTGCTGTTTGAGCCT
GTGCCGGTTCAAACTTTATC

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase

Number of amino acids: Translated: 138; Mature: 138

Protein sequence:

>138_residues
MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVD
DAYEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA

Sequences:

>Translated_138_residues
MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVD
DAYEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA
>Mature_138_residues
MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVD
DAYEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family

Homologues:

Organism=Homo sapiens, GI118402586, Length=148, Percent_Identity=39.8648648648649, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787940, Length=127, Percent_Identity=67.7165354330709, Blast_Score=191, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=31.5789473684211, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=44.6043165467626, Blast_Score=112, Evalue=3e-26,
Organism=Drosophila melanogaster, GI20129743, Length=150, Percent_Identity=40, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19920404, Length=139, Percent_Identity=29.4964028776978, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LGUL_NEIMA (P0A0T2)

Other databases:

- EMBL:   AL157959
- PIR:   G81211
- RefSeq:   YP_002343396.1
- ProteinModelPortal:   P0A0T2
- SMR:   P0A0T2
- EnsemblBacteria:   EBNEIT00000000165
- GeneID:   907300
- GenomeReviews:   AL157959_GR
- KEGG:   nma:NMA2147
- GeneTree:   EBGT00050000021776
- HOGENOM:   HBG704761
- OMA:   PGPMKHG
- ProtClustDB:   CLSK877504
- BioCyc:   NMEN122587:NMA2147-MONOMER
- BRENDA:   4.4.1.5
- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146
- TIGRFAMs:   TIGR00068

Pfam domain/function: PF00903 Glyoxalase

EC number: =4.4.1.5

Molecular weight: Translated: 15669; Mature: 15669

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00934 GLYOXALASE_I_1; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHN
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEECC
WDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYK
CCCCEEECCCCCEEEEEEECHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEECCCCCE
IEFIQKKSGDDSVAYQTA
EEEEEECCCCCCEEEECC
>Mature Secondary Structure
MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHN
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEECC
WDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYK
CCCCEEECCCCCEEEEEEECHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEECCCCCE
IEFIQKKSGDDSVAYQTA
EEEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919