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The map label for this gene is cysB [H]

Identifier: 161870688

GI number: 161870688

Start: 1812667

End: 1813542

Strand: Direct

Name: cysB [H]

Synonym: NMCC_1760

Alternate gene names: 161870688

Gene position: 1812667-1813542 (Clockwise)

Preceding gene: 161870684

Following gene: 161870690

Centisome position: 84.18

GC content: 52.85

Gene sequence:

>876_bases
TTGTTTACTTCGCAACCCGGCATCTCCAAACAAATCAAATTGCTGGAAGAAGAAATCGGCATTCAGATTTTTATCCGCAG
CGGCAAGCGCGTGGTTTCGGTCTCGCAGCCGGGCAAGGTGGTTTTGGATATTGCAGAACGTATTTTGCGCGATGTTCAGA
ACATTAAAAATATCGGCAGCGAGTTTACCGGACAGGACAGCGGTTCGCTGACGGTTGCCACGACGCATACTCAGGCACGC
TATGCCCTGCCCTTGATTGTTGCCGATTTTGTGAAACGCTATCCGAAAGTCAACCTGACCATCAAACAGGGGAGCCCTGC
CGCCATCGCCCAAATGGTTACTTCGGGAGAGTCTGATTTGGCAATTGTTACGGAACGTATAGACGACCACCCCGAACTGG
GAAGACTTTCCTGCTACGACTGGACACACGCGGTTATCGTACCGAACGACCACCCCTTGCTCGAATGCAGAAACCCCCTC
CGTATTGAAGATTTGGCGAGGTTTCCGCTGATTACTTATGAATTTGCATTCAATGCGGGCAGCAGCATCGCGCGGGCATT
TTCCAAAGCCCGTTTGGAACAGCCGGATGTCGCATTGGCGGCGGCGGATACGGACGTATTGAAGACTTATGTGCGCTTGG
GTTTGGGCGTGGGACTGATGGCGAAAATGGCGTACAACCCGGATACGGACGGCGATTTGCAGCTTGTGGATGCGGCACAC
CTGTTCGAGCCGTCGCCGACGTGGATTGCTTTGCGCAGCGATACTTATTTGCGCGGATATGCCTACGACTTTATCCAAGC
GTTTGCGCCGCACCTGACACGCGAGAAGGTGGATAGGATTCTGTACACGCCCATCAGCGAGGATTTTTCGATTTAG

Upstream 100 bases:

>100_bases
CGACACAGACCCACACCGACCCGCCATGAAATTACAACAATTGAAATACGCCTTAGAAGTTTACCGGCACAACCTGAACG
TTTCCGAAGCGGCCGAAGCC

Downstream 100 bases:

>100_bases
GCGGCTGCCGGTTTTCAGACGGCACTTTGCGGCAGATACAACAAACAGGACAGATGTTTTCGTCTGCCCTGTGTTTATTG
AGAATGCCGTCTGAAATGTT

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MFTSQPGISKQIKLLEEEIGIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHTQAR
YALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGRLSCYDWTHAVIVPNDHPLLECRNPL
RIEDLARFPLITYEFAFNAGSSIARAFSKARLEQPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAH
LFEPSPTWIALRSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI

Sequences:

>Translated_291_residues
MFTSQPGISKQIKLLEEEIGIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHTQAR
YALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGRLSCYDWTHAVIVPNDHPLLECRNPL
RIEDLARFPLITYEFAFNAGSSIARAFSKARLEQPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAH
LFEPSPTWIALRSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI
>Mature_291_residues
MFTSQPGISKQIKLLEEEIGIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHTQAR
YALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGRLSCYDWTHAVIVPNDHPLLECRNPL
RIEDLARFPLITYEFAFNAGSSIARAFSKARLEQPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAH
LFEPSPTWIALRSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=278, Percent_Identity=50.7194244604317, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1788296, Length=279, Percent_Identity=41.2186379928315, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI157672245, Length=230, Percent_Identity=30.4347826086957, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790399, Length=241, Percent_Identity=24.4813278008299, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788887, Length=230, Percent_Identity=24.7826086956522, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788481, Length=219, Percent_Identity=24.2009132420091, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32293; Mature: 32293

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTSQPGISKQIKLLEEEIGIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGS
CCCCCCCCHHHHHHHHHHCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
EFTGQDSGSLTVATTHTQARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDL
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCE
AIVTERIDDHPELGRLSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAG
EEEEECCCCCCCCCCEEEECCEEEEECCCCCCHHCCCCCCCHHHHHHCCEEEEEEECCCC
SSIARAFSKARLEQPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAH
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEHHH
LFEPSPTWIALRSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI
HCCCCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
>Mature Secondary Structure
MFTSQPGISKQIKLLEEEIGIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGS
CCCCCCCCHHHHHHHHHHCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
EFTGQDSGSLTVATTHTQARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDL
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCE
AIVTERIDDHPELGRLSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAG
EEEEECCCCCCCCCCEEEECCEEEEECCCCCCHHCCCCCCCHHHHHHCCEEEEEEECCCC
SSIARAFSKARLEQPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAH
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEHHH
LFEPSPTWIALRSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI
HCCCCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]