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The map label for this gene is yafV [C]

Identifier: 161870635

GI number: 161870635

Start: 1742265

End: 1743077

Strand: Reverse

Name: yafV [C]

Synonym: NMCC_1705

Alternate gene names: 161870635

Gene position: 1743077-1742265 (Counterclockwise)

Preceding gene: 161870637

Following gene: 161870632

Centisome position: 80.94

GC content: 61.87

Gene sequence:

>813_bases
ATGGACAAAATCAGAGTTGCCGCCGTGCAGATGGTGTCGGGCGTGTCGCCGGAAACCAACGTCGCCGCCATGAAACGCCT
GGTCGCACGGGCGGCGGAGCAGGGTGCGGATTGGGTGCTGCTGCCCGAATATTGGGTGCTGATGGGCGCAAACGATACCG
ACAAACTCGCGCTTGCCGAGCCTTTGGGCGGCGGACGCTTTCAGACGGCATTGAGCGAAACGGCGAAAGAATGCGGCGTG
GTGCTGTTCGGCGGAACCGTGCCGCTGCAAAGCCCCGAGGCGGGCAAGGTCATGAATACTTTATTGGTGTACGGGCGCGA
CGGCAAACGGACGGGGCTGTACCACAAAATGCACCTCTTCGGCTTTTCCGGTTTGGGCGAACGCTATGCCGAAGCCGATA
CCATCCGCGCGGGCGGGGAAGTGCCGCACTTGTCGGCAGAGGGCGTGCCGGTGGCGGCGGGCATTTGTTACGATGTCCGC
TTTTCCGAATTTTTCCGCCGCCAGCTGCCGTTTGACGTATTGATGCTGCCCGCTGCGTTTACGCACACGACGGGCAAGGC
GCATTGGGAGCTGCTGCTGCGCGCGCGTGCCGTCGAAAACCAATGTTACGTCGTGGCGGCGGCACAGGGCGGTTTGCACG
AAAACGGACGGCGCACGTTCGGACACAGCATGATTGTCGATCCGTGGGGCGACGTATTGGACGTATTGCCCGAAGGCGAA
GGCGTTGTTACGGCAGACATCGATGCCAACCGCCTGAACAGCGTCCGCAACCGCCTGCCCGCCTTGAAATACCGGGTTTT
GGATGCCGTCTGA

Upstream 100 bases:

>100_bases
GGTTCAAAATGGGCATTTGTGTAAACGGTTTTCGCAAAGTACCGTCATGGTAGCCTATCGGCGGAATATGCCGCAAGGTC
GGCAGGAAAAAGGAGAAGAA

Downstream 100 bases:

>100_bases
AGGTTCAGACGGCATCGGTGCCGGGGAATCAGCGGGCTGTAGGATGGGCTTCAGCCTGCCATTTCCATCAATCCCACATT
TCTACCGTTTTCATCGAATC

Product: nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEYWVLMGANDTDKLALAEPLGGGRFQTALSETAKECGV
VLFGGTVPLQSPEAGKVMNTLLVYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGEVPHLSAEGVPVAAGICYDVR
FSEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRRTFGHSMIVDPWGDVLDVLPEGE
GVVTADIDANRLNSVRNRLPALKYRVLDAV

Sequences:

>Translated_270_residues
MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEYWVLMGANDTDKLALAEPLGGGRFQTALSETAKECGV
VLFGGTVPLQSPEAGKVMNTLLVYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGEVPHLSAEGVPVAAGICYDVR
FSEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRRTFGHSMIVDPWGDVLDVLPEGE
GVVTADIDANRLNSVRNRLPALKYRVLDAV
>Mature_270_residues
MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEYWVLMGANDTDKLALAEPLGGGRFQTALSETAKECGV
VLFGGTVPLQSPEAGKVMNTLLVYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGEVPHLSAEGVPVAAGICYDVR
FSEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRRTFGHSMIVDPWGDVLDVLPEGE
GVVTADIDANRLNSVRNRLPALKYRVLDAV

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=272, Percent_Identity=36.3970588235294, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI297632348, Length=271, Percent_Identity=36.5313653136531, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI5031947, Length=271, Percent_Identity=36.5313653136531, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI297632350, Length=271, Percent_Identity=36.5313653136531, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI297632346, Length=193, Percent_Identity=29.5336787564767, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786412, Length=230, Percent_Identity=27.8260869565217, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556280, Length=268, Percent_Identity=37.3134328358209, Blast_Score=167, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6322335, Length=295, Percent_Identity=30.8474576271186, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323383, Length=279, Percent_Identity=32.258064516129, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI17933642, Length=268, Percent_Identity=37.3134328358209, Blast_Score=160, Evalue=7e-40,
Organism=Drosophila melanogaster, GI21355835, Length=269, Percent_Identity=33.0855018587361, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29291; Mature: 29291

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEYWVLMGANDTDKLALAE
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCEEEEC
PLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLLVYGRDGKRTGLYHKMHLF
CCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHEEECCCCCCCCCEEEEEEE
GFSGLGERYAEADTIRAGGEVPHLSAEGVPVAAGICYDVRFSEFFRRQLPFDVLMLPAAF
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECHHHHHHHHHCCHHEEECCHHH
THTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRRTFGHSMIVDPWGDVLDVLPEGE
HCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCHHHHHHHCCCCC
GVVTADIDANRLNSVRNRLPALKYRVLDAV
CEEEECCCHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEYWVLMGANDTDKLALAE
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCEEEEC
PLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLLVYGRDGKRTGLYHKMHLF
CCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHEEECCCCCCCCCEEEEEEE
GFSGLGERYAEADTIRAGGEVPHLSAEGVPVAAGICYDVRFSEFFRRQLPFDVLMLPAAF
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECHHHHHHHHHCCHHEEECCHHH
THTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRRTFGHSMIVDPWGDVLDVLPEGE
HCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCHHHHHHHCCCCC
GVVTADIDANRLNSVRNRLPALKYRVLDAV
CEEEECCCHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]