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The map label for this gene is amiC [H]

Identifier: 161870618

GI number: 161870618

Start: 1724474

End: 1725724

Strand: Direct

Name: amiC [H]

Synonym: NMCC_1687

Alternate gene names: 161870618

Gene position: 1724474-1725724 (Clockwise)

Preceding gene: 161870617

Following gene: 161870619

Centisome position: 80.08

GC content: 59.31

Gene sequence:

>1251_bases
ATGACCAAACTGACACGAAGACAAATCATCCGCCGCACCGCCGGCACACTGTTCGCCCTAAGCCCCATCGCATCCGCCGT
TGCCAAAACGGTACGCGCCCCGCAGTTTACCGCCGTACGGATATGGCCGTCGCACACCTACACCCGCCTGACGCTGGAAA
GCACCGCCGCGCTCAAATACCAACACTTCACGCTCGACAATCCGGGCAGGCTGGTCGTCGATATACAAAACGCGAACATC
AATACCGTATTGCACGGACTTTCCCAAAAAGTTATGGCGGACGACCCCTTTATCCGCAGCATACGCGCCGGTCAGAACAC
GCCCGACACCGTCCGCCTCGTCATCGATTTGAAACAGCCTTCCTACGCGCAAGTTTTTTCCCTCCCACCCGTCGGCGGCT
TTAAAAACCGCCTCGTCGTCGATCTCTATCCGCAAGGGATGGATGCCGACGATCCGATGATGGCGCTGCTCAACGGCAGC
CTGAACAAAACCCGTCGCGGCTCCCCTGAAGCCGACCTCGCTCAAAACACTCCCGCACAAACCGAACGCGGCAAAAACGG
GCGCAGACCCGTCATTATGATCGACCCCGGACACGGCGGCGAAGACCCCGGCGCCGTCAGCCCGGGCGGTTTGCAGGAAA
AACACGTCGTCCTCTCCATCGCCCGCGAAACCAAAAAACAATTGGAAGCCTTGGGTTACAACGTCTTTATGACGCGCAAC
GAAGACGTGTTCATCCCATTGGGCGTGCGTGTCGCCAAAGGGCGCGCACGGCGGGCGGACGTATTCGTCTCCATCCACGC
CGACGCTTTCACCAGCCCCTCCGCGCGCGGAACGGGGGTTTATATGCTGAACACCAAAGGCGCGACCAGCTCTGCCGCCA
AATTCTTGGAACAAACCCAAAATAATGCCGACGCGGTCGGCGGCGTACCGACCAGCGGCAACCGCAATGTCGATACCGCC
CTGCTCGACATGACCCAAACCGCCACGCTGCGCGACAGCCGCAAACTCGGCAAACTGGTGCTTGAAGAATTGGGCAGGCT
CAACCACTTGCACAAAGGCAGGGTGGACGAAGCCAATTTCGCCGTTTTGCGCGCGCCCGATATGCCGTCTATCCTGGTCG
AAACCGCCTTCCTGTCCAATCCTGCCGAAGAGAAGCTGCTGGGCAGCGAATCCTTCCGTCGGCAGTGCGCCCAATCCATT
GCCTCGGGTGTCCAACGCTACATCAATACATCCGTATTGAAGCGGGGTTGA

Upstream 100 bases:

>100_bases
CCCCGCCTGCCGACATCACCGCAACATTGACACACGACGGCGACGGCAGAAAATGCCTGCTGACCGCCCATACCGAACGA
GGACGCGAAAGCCTGCCGCT

Downstream 100 bases:

>100_bases
TTGAAAAGGCGGTAGTCGGATGAAGGCAGGCATAAACCTACTTCCGCCCCAAACCGCGTTTTTATGATGAAAGGTATGGA
TAAATTGAGATACCAACAGG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPSHTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANI
NTVLHGLSQKVMADDPFIRSIRAGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDADDPMMALLNGS
LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVLSIARETKKQLEALGYNVFMTRN
EDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSARGTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTA
LLDMTQTATLRDSRKLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESFRRQCAQSI
ASGVQRYINTSVLKRG

Sequences:

>Translated_416_residues
MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPSHTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANI
NTVLHGLSQKVMADDPFIRSIRAGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDADDPMMALLNGS
LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVLSIARETKKQLEALGYNVFMTRN
EDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSARGTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTA
LLDMTQTATLRDSRKLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESFRRQCAQSI
ASGVQRYINTSVLKRG
>Mature_415_residues
TKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPSHTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANIN
TVLHGLSQKVMADDPFIRSIRAGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDADDPMMALLNGSL
NKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVLSIARETKKQLEALGYNVFMTRNE
DVFIPLGVRVAKGRARRADVFVSIHADAFTSPSARGTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTAL
LDMTQTATLRDSRKLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESFRRQCAQSIA
SGVQRYINTSVLKRG

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=417, Percent_Identity=51.5587529976019, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1788776, Length=226, Percent_Identity=42.9203539823009, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1790611, Length=237, Percent_Identity=36.2869198312236, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 45300; Mature: 45169

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPSHTYTRLTLESTAALKY
CCCHHHHHHHHHHHCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCEEEEE
QHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIRAGQNTPDTVRLVIDLKQP
EEEEECCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEEECCC
SYAQVFSLPPVGGFKNRLVVDLYPQGMDADDPMMALLNGSLNKTRRGSPEADLAQNTPAQ
CCEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHCCCCCH
TERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVLSIARETKKQLEALGYNVFMTRN
HHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECC
EDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSARGTGVYMLNTKGATSSAAKFLEQTQ
CCEEEECCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHC
NNADAVGGVPTSGNRNVDTALLDMTQTATLRDSRKLGKLVLEELGRLNHLHKGRVDEANF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
AVLRAPDMPSILVETAFLSNPAEEKLLGSESFRRQCAQSIASGVQRYINTSVLKRG
EEEECCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPSHTYTRLTLESTAALKY
CCHHHHHHHHHHHCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCEEEEE
QHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIRAGQNTPDTVRLVIDLKQP
EEEEECCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEEECCC
SYAQVFSLPPVGGFKNRLVVDLYPQGMDADDPMMALLNGSLNKTRRGSPEADLAQNTPAQ
CCEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHCCCCCH
TERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVLSIARETKKQLEALGYNVFMTRN
HHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECC
EDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSARGTGVYMLNTKGATSSAAKFLEQTQ
CCEEEECCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHC
NNADAVGGVPTSGNRNVDTALLDMTQTATLRDSRKLGKLVLEELGRLNHLHKGRVDEANF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
AVLRAPDMPSILVETAFLSNPAEEKLLGSESFRRQCAQSIASGVQRYINTSVLKRG
EEEECCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]