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The map label for this gene is pldA [C]

Identifier: 161870609

GI number: 161870609

Start: 1709110

End: 1710237

Strand: Reverse

Name: pldA [C]

Synonym: NMCC_1678

Alternate gene names: 161870609

Gene position: 1710237-1709110 (Counterclockwise)

Preceding gene: 161870610

Following gene: 161870608

Centisome position: 79.42

GC content: 54.34

Gene sequence:

>1128_bases
ATGAATACACGGAATATGCGCTATATTCTTTTGACAGGACTGTTGCCGACGGTATCCGCTTTTGGAGAGACCGCGCTGCA
ATGCGCCGCTTTGACGGACAATGTTACGCGTTTGGCGTGTTACGACAGGATTTTTGCGGCACAGCTTCCGTCTTCGGCAG
GGCAGGAAGGGCAGGAGTCGAAAGCCGTACTCAATCTGACGGAAACCGTCCGCAGCAGCCTGGATAAGGGCGAGGCGGTC
ATTGTTGTTGAAAAAGGCGGGGATGCGCTTCCTGCCGACAGTGCGGGCGAAACCGCCGACATCTATACGCCTTTGAGCCT
GATGTACGACTTGGACAAAAACGATTTGCGCGGGCTGTTGGGCGTACGCGAACACAATCCGATGTACCTTATGCCGCTCT
GGTACAACAATTCGCCCAACTATGCCCCGGGTTCGCCGACGCGCGGTACGACTGTACAGGAAAAATTCGGACAGCAGAAA
CGTGCGGAAACCAAATTGCAGGTTTCGTTCAAAAGCAAAATTGCCGAAGATTTGTTTAAAACCCGCGCGGATCTGTGGTT
CGGCTACACCCAAAGATCCGATTGGCAGATTTACAACCAAGGCAGGAAATCCGCGCCGTTCCGCAATACGGATTACAAAC
CTGAAATTTTCCTGACCCAGCCTGTGAAGGCGGATTTGCCGTTCGGCGGCAGGCTGCGTATGCTCGGTGCGGGTTTTGTC
CACCAGTCCAACGGACAGAGCCGTCCCGAATCGCGTTCGTGGAACAGGATTTACGCCATGGCAGGCATGGAATGGGGCAA
ATTGACGGTGATTCCGCGCGTGTGGGTGCGTGCGTTCGATCAGAGCGGCGATAAAAACGACAATCCCGATATTGCCGACT
ATATGGGGTATGGCGACGTGAAGCTGCAGTACCGCCTGAACGACAGGCAGAATGTGTATTCCGTATTGCGCTACAACCCC
AAAACGGGCTACGGCGCGATTGAAGCCGCCTACACGTTTCCGATTAAGGGCAAACTCAAAGGCGTGGTACGCGGATTCCA
CGGTTACGGCGAGAGCCTGATCGACTACAACCACAAGCAGAACGGTATCGGTATCGGGTTGATGTTCAACGACTTGGACG
GCATCTGA

Upstream 100 bases:

>100_bases
CGGGGGCTAAATCCCGAAATGGCGGAAAGGGTGCGGTTTTTTATCCGAATCCGCTATAAAATGCCGTTTGAAAACCAATA
TGCCGACAATGGGGGCGGAG

Downstream 100 bases:

>100_bases
ACCGCGTGTTCAGACGGTATATCAAGTTGCCGTGCCGTCTGAAGCCGCCGGCGGTTTGGCGGGGCGGGATAAAAATTTCG
TTTGAATCCGGGCAAACGCG

Product: phospholipase A1, putative

Products: NA

Alternate protein names: Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MNTRNMRYILLTGLLPTVSAFGETALQCAALTDNVTRLACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAV
IVVEKGGDALPADSAGETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQEKFGQQK
RAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTDYKPEIFLTQPVKADLPFGGRLRMLGAGFV
HQSNGQSRPESRSWNRIYAMAGMEWGKLTVIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNP
KTGYGAIEAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI

Sequences:

>Translated_375_residues
MNTRNMRYILLTGLLPTVSAFGETALQCAALTDNVTRLACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAV
IVVEKGGDALPADSAGETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQEKFGQQK
RAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTDYKPEIFLTQPVKADLPFGGRLRMLGAGFV
HQSNGQSRPESRSWNRIYAMAGMEWGKLTVIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNP
KTGYGAIEAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI
>Mature_375_residues
MNTRNMRYILLTGLLPTVSAFGETALQCAALTDNVTRLACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAV
IVVEKGGDALPADSAGETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQEKFGQQK
RAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTDYKPEIFLTQPVKADLPFGGRLRMLGAGFV
HQSNGQSRPESRSWNRIYAMAGMEWGKLTVIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNP
KTGYGAIEAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=218, Percent_Identity=39.4495412844037, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 41995; Mature: 41995

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTRNMRYILLTGLLPTVSAFGETALQCAALTDNVTRLACYDRIFAAQLPSSAGQEGQES
CCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCC
KAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSAGETADIYTPLSLMYDLDKNDLRGLL
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCHHEEECCHHHHHHHH
GVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQEKFGQQKRAETKLQVSFKSKIAEDLFK
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHCHHHHCCCEEEEHHHHHHHHHHHH
TRADLWFGYTQRSDWQIYNQGRKSAPFRNTDYKPEIFLTQPVKADLPFGGRLRMLGAGFV
HHHHHEEECCCCCCCHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCEE
HQSNGQSRPESRSWNRIYAMAGMEWGKLTVIPRVWVRAFDQSGDKNDNPDIADYMGYGDV
ECCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHCCCCCCCCCCHHHHCCCCCE
KLQYRLNDRQNVYSVLRYNPKTGYGAIEAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQ
EEEEEECCHHHHHHHHEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCHHHCCCCCC
NGIGIGLMFNDLDGI
CCEEEEEEECCCCCC
>Mature Secondary Structure
MNTRNMRYILLTGLLPTVSAFGETALQCAALTDNVTRLACYDRIFAAQLPSSAGQEGQES
CCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCC
KAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSAGETADIYTPLSLMYDLDKNDLRGLL
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCHHEEECCHHHHHHHH
GVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQEKFGQQKRAETKLQVSFKSKIAEDLFK
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHCHHHHCCCEEEEHHHHHHHHHHHH
TRADLWFGYTQRSDWQIYNQGRKSAPFRNTDYKPEIFLTQPVKADLPFGGRLRMLGAGFV
HHHHHEEECCCCCCCHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCEE
HQSNGQSRPESRSWNRIYAMAGMEWGKLTVIPRVWVRAFDQSGDKNDNPDIADYMGYGDV
ECCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHCCCCCCCCCCHHHHCCCCCE
KLQYRLNDRQNVYSVLRYNPKTGYGAIEAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQ
EEEEEECCHHHHHHHHEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCHHHCCCCCC
NGIGIGLMFNDLDGI
CCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]