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The map label for this gene is eda [H]

Identifier: 161870268

GI number: 161870268

Start: 1312656

End: 1313294

Strand: Direct

Name: eda [H]

Synonym: NMCC_1309

Alternate gene names: 161870268

Gene position: 1312656-1313294 (Clockwise)

Preceding gene: 161870267

Following gene: 161870270

Centisome position: 60.96

GC content: 61.66

Gene sequence:

>639_bases
ATGTCCAAACTGACCCCACGCGAAATCCTGACCGCCGGCGCAGTTGTGCCTGTGATGGCGATTGACGACTTAAGCACCGC
CGTCGATTTGTCCTACGCCCTTGTCGAAGGCGGCATCCCTACCCTCGAAATTACCCTGCGTACACCCGTCGGCCTCGACG
CCATCCGCCTGATTGCCAAAGAAGTGCCCAACGCCATCGTCGGCGCGGGTACGGTAACCAATCCCGAACAGCTCAAAGCC
GTCGAAGACGCAGGCGCGGTTTTCGCCATCAGCCCGGGGCTGCATGAATCCCTCGCCAAAGCCGGCCACAACAGCGGCAT
CCCCCTGATTCCCGGTGTTGCCACCCCGGGCGAAATCCAACTGGCTTTGGAACACGGCATCGACACCCTCAAACTCTTCC
CCGCCGAAGTCGTCGGCGGCAAAGCCATGCTCAAAGCCCTGTACGGCCCTTACGCCGATGTTCGCTTCTGCCCGACAGGC
GGCATCAGCCTCGCCACCGCGCCCGAGTACTTGGCACTGCCCAACGTCCTGTGCGTCGGCGGCTCTTGGCTGACACCGAA
AGAAGCCGTGAAAAACAAAGACTGGGACACCATCACCCGCCTCGCCAAAGAAGCGGCGGCGTTGAAACCCAAAGCCTGA

Upstream 100 bases:

>100_bases
TAAGCAACTTCCCCCCATTTTTCAAAAAGGCCGTCTGAAAACCTAGCCCCTTCCTGTTTGACCTCAACAAAAATGCCCCT
ATCCCACTTGGAGAACTGAA

Downstream 100 bases:

>100_bases
TTCGCATCGTAAAAATGCCGTCTGAAAAACCTTTCCCGTTTCAGACGGCATTTTGCCGATTGAGGGCACAGTCGGCATAC
ACGGCAGCACTGATCAGACA

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSKLTPREILTAGAVVPVMAIDDLSTAVDLSYALVEGGIPTLEITLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKA
VEDAGAVFAISPGLHESLAKAGHNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
GISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA

Sequences:

>Translated_212_residues
MSKLTPREILTAGAVVPVMAIDDLSTAVDLSYALVEGGIPTLEITLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKA
VEDAGAVFAISPGLHESLAKAGHNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
GISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA
>Mature_211_residues
SKLTPREILTAGAVVPVMAIDDLSTAVDLSYALVEGGIPTLEITLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAV
EDAGAVFAISPGLHESLAKAGHNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTGG
ISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=203, Percent_Identity=56.1576354679803, Blast_Score=213, Evalue=7e-57,
Organism=Escherichia coli, GI48994954, Length=142, Percent_Identity=32.3943661971831, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 21933; Mature: 21802

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLTPREILTAGAVVPVMAIDDLSTAVDLSYALVEGGIPTLEITLRTPVGLDAIRLIAK
CCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHH
EVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAGHNSGIPLIPGVATPGEIQ
HCCCCEECCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCEE
LALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTGGISLATAPEYLALPNVLCVG
EEHHCCCCEEEECHHHHHCHHHHHHHHHCCCCCEEECCCCCEEECCCCCHHHCCCEEEEC
GSWLTPKEAVKNKDWDTITRLAKEAAALKPKA
CCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKLTPREILTAGAVVPVMAIDDLSTAVDLSYALVEGGIPTLEITLRTPVGLDAIRLIAK
CCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHH
EVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAGHNSGIPLIPGVATPGEIQ
HCCCCEECCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCEE
LALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTGGISLATAPEYLALPNVLCVG
EEHHCCCCEEEECHHHHHCHHHHHHHHHCCCCCEEECCCCCEEECCCCCHHHCCCEEEEC
GSWLTPKEAVKNKDWDTITRLAKEAAALKPKA
CCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]