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The map label for this gene is truB [H]

Identifier: 161870245

GI number: 161870245

Start: 1287087

End: 1288037

Strand: Direct

Name: truB [H]

Synonym: NMCC_1286

Alternate gene names: 161870245

Gene position: 1287087-1288037 (Clockwise)

Preceding gene: 161870244

Following gene: 161870246

Centisome position: 59.77

GC content: 56.57

Gene sequence:

>951_bases
ATGCCCATGCCGTCCGAACATCGAACCATGAATACAGGCAAACCCCAAAAACGTGCCGTCAACGGTGTTTTGCTCTTGGA
CAAACCCGAAGGCCTTTCCAGCAACACCGCGCTGCAAAAAGCGCGGCGTTTGTTCCGTGCCGAAAAAGCCGGACATACCG
GCGTGCTCGATCCTTTGGCAACCGGACTTTTGCCCGTCTGCTTCGGCGAAGCGACCAAGTTCTCCCAATACCTGCTGGAT
GCCGACAAAGCCTACACCGCCACGCTGAAACTCGGCGAAGCCAGCAGCACGGGTGATGCCGAAGGCGAAATCGTTGCCAC
CGCCCGTGCCGATATTTCCTTAGCCGAATTTCAGACGGCCTGCCAAGCACTGACAGGCAACATCCGCCAAGTGCCACCAA
TGTTTTCCGCGCTCAAACACGAAGGTAAACCGCTGTACGAATACGCGCGCAAAGGCATCGTCATCGAACGCAAAGCGCGC
GACATTACCGTTTACGCCATCGATATTGCCGAATTTGATGCGCCCAAAGCCGTGATAAGCGTGCGTTGCAGCAAAGGCAC
CTATATCCGCACCCTCAGCGAAGACATCGCCAAACACATCGGCACGTTCGCCCACCTGACCGCCCTGCGCCGCACCGAAA
CCGCCGGTTTTACCATCGCCCAAAGCCACACGCTCGAAGCCTTGGCAAACTTGGACGAAACCGGACGCGACAGCTTGCTG
CTGCCCTGCGACGTATTGGTTGCCCATTTTCCCCAAACCGTTTTAAACGATTATGCCGTCCATATGCTCCAATGCGGGCA
ACGGCCGCGTTTCGATGAAGACCTGACTTCCGATACGCCGGTACGCGTTTACACGGAAAACGGCCACTTTGTCGGCCTGG
CGGAATATCAAAAAGAAATATGCCGTCTGAAAGCCTTGCGCCTGATGAACACGGCGGCATCCGCCGTCTGA

Upstream 100 bases:

>100_bases
AGCTGCATTTCAAATACGACGAATCGTTGGAACGCGGTTTGAACCTGTCCGCCCTTATCGATCAAGTGGCGGCGGAAAAA
CCGGTTGAAGACTGACGGAT

Downstream 100 bases:

>100_bases
ACGGCAGCTGTGTTTGAATAATATGTTGATATTTCCGCAAAATCCCGACACACTCGGACACCCGCCCCGCTTATCGCAAC
TTTGCGAACGCCCCCGGAAA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MPMPSEHRTMNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHTGVLDPLATGLLPVCFGEATKFSQYLLD
ADKAYTATLKLGEASSTGDAEGEIVATARADISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKAR
DITVYAIDIAEFDAPKAVISVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEALANLDETGRDSLL
LPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFDEDLTSDTPVRVYTENGHFVGLAEYQKEICRLKALRLMNTAASAV

Sequences:

>Translated_316_residues
MPMPSEHRTMNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHTGVLDPLATGLLPVCFGEATKFSQYLLD
ADKAYTATLKLGEASSTGDAEGEIVATARADISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKAR
DITVYAIDIAEFDAPKAVISVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEALANLDETGRDSLL
LPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFDEDLTSDTPVRVYTENGHFVGLAEYQKEICRLKALRLMNTAASAV
>Mature_315_residues
PMPSEHRTMNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHTGVLDPLATGLLPVCFGEATKFSQYLLDA
DKAYTATLKLGEASSTGDAEGEIVATARADISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARD
ITVYAIDIAEFDAPKAVISVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEALANLDETGRDSLLL
PCDVLVAHFPQTVLNDYAVHMLQCGQRPRFDEDLTSDTPVRVYTENGHFVGLAEYQKEICRLKALRLMNTAASAV

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=249, Percent_Identity=33.3333333333333, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI4503337, Length=302, Percent_Identity=26.158940397351, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI215599015, Length=302, Percent_Identity=26.158940397351, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI2367200, Length=303, Percent_Identity=43.2343234323432, Blast_Score=240, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17553978, Length=302, Percent_Identity=24.8344370860927, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=278, Percent_Identity=27.3381294964029, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324037, Length=191, Percent_Identity=29.3193717277487, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364189, Length=291, Percent_Identity=25.4295532646048, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364187, Length=291, Percent_Identity=25.4295532646048, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364185, Length=291, Percent_Identity=25.4295532646048, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364183, Length=291, Percent_Identity=25.4295532646048, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI62471759, Length=291, Percent_Identity=25.4295532646048, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17975520, Length=291, Percent_Identity=25.4295532646048, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 34548; Mature: 34417

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMPSEHRTMNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHTGVLDPLA
CCCCCCCCCCCCCCCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHH
TGLLPVCFGEATKFSQYLLDADKAYTATLKLGEASSTGDAEGEIVATARADISLAEFQTA
HHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHH
CQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDIAEFDAPKAVIS
HHHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCEEEEECCCEEEEEEEEEECCCCCCEEEE
VRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEALANLDETGRDSLL
EEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCEE
LPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFDEDLTSDTPVRVYTENGHFVGLAEYQKEI
CHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEHHHHHHHH
CRLKALRLMNTAASAV
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PMPSEHRTMNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHTGVLDPLA
CCCCCCCCCCCCCCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHH
TGLLPVCFGEATKFSQYLLDADKAYTATLKLGEASSTGDAEGEIVATARADISLAEFQTA
HHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHH
CQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDIAEFDAPKAVIS
HHHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCEEEEECCCEEEEEEEEEECCCCCCEEEE
VRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEALANLDETGRDSLL
EEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCEE
LPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFDEDLTSDTPVRVYTENGHFVGLAEYQKEI
CHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEHHHHHHHH
CRLKALRLMNTAASAV
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]