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The map label for this gene is rlmL [H]

Identifier: 161870237

GI number: 161870237

Start: 1279323

End: 1280387

Strand: Reverse

Name: rlmL [H]

Synonym: NMCC_1278

Alternate gene names: 161870237

Gene position: 1280387-1279323 (Counterclockwise)

Preceding gene: 161870239

Following gene: 161870235

Centisome position: 59.46

GC content: 51.17

Gene sequence:

>1065_bases
ATGGCTTCGTATGACAAGATAAGCGGTGGATGGTATAGGGTTTGCCCGAAACGGTCAAGCAATCGGGCGTTAATTACAGT
AAAATTGCCGTTTTCCACTTTATTCCGCCTGAAACCAATGACCGACATCACACCGTTTGCCAACCGCTTGGGCAAAAACA
TCAAACACCTTATGAAATGGGCGAAACGCAACGGCATCGAAGCCTGGCGCATTTACGACCGCGACATTCCCCAATTCCCT
TTTGCCGCCGATGTTTACGGTGATCGAATCCACCTTCAGGAATACGATACCGGCTGGCTGATGCAGCCCGAAGAATACGA
AGCGTGGCTTGCCGAAGTATTGGAAGCCGTCGTTTTCGTTACCGGTTTTGCGCCCGAACAAATCCACCTCAAACGCCGCG
AACGTCAAAAAGGTTTGCAGCAATACGAGAAAACCGGCAAAACCGGCGATGATTTTGTCATCACCGAAAACGGCCGCAAG
TTTTGGGTCAACCTCGACAAATACCTCGATACCGGCCTTTTCCTCGACCACCGCAACACGCGCAAAAAAGTCGGCGAAAC
CGCAGCGGGCAAACGCTTCCTCAACCTGTTTTCCTACACAGGCAGCTTCACCGTCTACGCCGCCACCGGCGGCGCGGCAT
CCAGTGAAACCGTCGATTTGTCCAACACTTATCTCGATTGGGCGAAGCGCAATTTTGAATTGAACGGCATCGATACGGAA
CGGCACAAAATCGTCCGCGCCGACGTGTTCCAATATCTTCAGACGGCATATGGGGAAGGCAGGCGGTTTGACCTCATTGT
GATGGATCCGCCCAGTTTTTCCAACAGCAAGAAAATGTCCGACATCCTCGACATTCAGCGCGACCACAAAAAGCTGATTG
ACCGCGCGGTGAAGCTGCTCGCTTCAGACGGCATTTTGTACTTTTCCAACAACCTGCGCAGCTTCGTCTTAGACGACTTG
GTATCGGAACAATACGCCGTCAAAGACATTTCCAAACAATCCGTTCCCGAAGATTTCCGCAACAAAAAAATCCATAGGTG
CTGGGAAATCCGCCACGAATCTTAA

Upstream 100 bases:

>100_bases
TGCTTGTGGTTTACATCGACGGCAACCTGCACAGATACGCTTTGCAAAATCAAAGCAACAGAAGAAACGCGCCCGTAAGG
GCGGTTTAGGTTGGAAGGCG

Downstream 100 bases:

>100_bases
GGGTGCGAAAAAGCCGCCGGAATCTTCCGGCGGCTTTTTCGATTGCGCTTTATCAGGCGATAGAGGCTTCGACAAAGGCG
GTCAGCTGACCTTTTGCCAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MASYDKISGGWYRVCPKRSSNRALITVKLPFSTLFRLKPMTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFP
FAADVYGDRIHLQEYDTGWLMQPEEYEAWLAEVLEAVVFVTGFAPEQIHLKRRERQKGLQQYEKTGKTGDDFVITENGRK
FWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSYTGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTE
RHKIVRADVFQYLQTAYGEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDRAVKLLASDGILYFSNNLRSFVLDDL
VSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHES

Sequences:

>Translated_354_residues
MASYDKISGGWYRVCPKRSSNRALITVKLPFSTLFRLKPMTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFP
FAADVYGDRIHLQEYDTGWLMQPEEYEAWLAEVLEAVVFVTGFAPEQIHLKRRERQKGLQQYEKTGKTGDDFVITENGRK
FWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSYTGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTE
RHKIVRADVFQYLQTAYGEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDRAVKLLASDGILYFSNNLRSFVLDDL
VSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHES
>Mature_353_residues
ASYDKISGGWYRVCPKRSSNRALITVKLPFSTLFRLKPMTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPF
AADVYGDRIHLQEYDTGWLMQPEEYEAWLAEVLEAVVFVTGFAPEQIHLKRRERQKGLQQYEKTGKTGDDFVITENGRKF
WVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSYTGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTER
HKIVRADVFQYLQTAYGEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDRAVKLLASDGILYFSNNLRSFVLDDLV
SEQYAVKDISKQSVPEDFRNKKIHRCWEIRHES

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=316, Percent_Identity=45.5696202531646, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI145693111, Length=272, Percent_Identity=32.7205882352941, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 41139; Mature: 41008

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASYDKISGGWYRVCPKRSSNRALITVKLPFSTLFRLKPMTDITPFANRLGKNIKHLMKW
CCCCCCCCCCEEEECCCCCCCCEEEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AKRNGIEAWRIYDRDIPQFPFAADVYGDRIHLQEYDTGWLMQPEEYEAWLAEVLEAVVFV
HHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
TGFAPEQIHLKRRERQKGLQQYEKTGKTGDDFVITENGRKFWVNLDKYLDTGLFLDHRNT
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEHHHHHCCCEEEECCCH
RKKVGETAAGKRFLNLFSYTGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTE
HHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCHHHHHHHHCCCEECCCCHH
RHKIVRADVFQYLQTAYGEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDRAVKLL
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ASDGILYFSNNLRSFVLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHES
HCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASYDKISGGWYRVCPKRSSNRALITVKLPFSTLFRLKPMTDITPFANRLGKNIKHLMKW
CCCCCCCCCEEEECCCCCCCCEEEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AKRNGIEAWRIYDRDIPQFPFAADVYGDRIHLQEYDTGWLMQPEEYEAWLAEVLEAVVFV
HHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
TGFAPEQIHLKRRERQKGLQQYEKTGKTGDDFVITENGRKFWVNLDKYLDTGLFLDHRNT
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEHHHHHCCCEEEECCCH
RKKVGETAAGKRFLNLFSYTGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTE
HHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCHHHHHHHHCCCEECCCCHH
RHKIVRADVFQYLQTAYGEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDRAVKLL
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ASDGILYFSNNLRSFVLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHES
HCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA