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The map label for this gene is aspA [H]

Identifier: 161870127

GI number: 161870127

Start: 1156874

End: 1158319

Strand: Direct

Name: aspA [H]

Synonym: NMCC_1166

Alternate gene names: 161870127

Gene position: 1156874-1158319 (Clockwise)

Preceding gene: 161870126

Following gene: 161870130

Centisome position: 53.72

GC content: 51.94

Gene sequence:

>1446_bases
ATGGCGCATTTTTTCAATATTAATTATCCGACTTACAAAGGTAAAAACATGACTGTCCGTATCGAACACGATTTATTGGG
CGACCGCGAGATTCCCGCCGAAGTGTATTGGGGCATCCACACTCTGCGCGCCATTGAAAACTTTAAAATCTCCACACAAA
AAATTTCCGACGTGCCGCAGTTTGTCCGCAGTATGGTGATGGTGAAAAAAGCAACCGCGCAGGCAAACGGCGAATTGGGT
GCAGTAAAACCCGAAATCGCCGCCGCCATTGAAAAGGCTTGCGACGAAGTTCTGCTGAACAACCGCTGCCTCGACCAATT
CCCCTCCGACGTGTATCAGGGCGGTGCGGGAACTTCGGTCAATATGAACACCAACGAAGTCATCGCCAACCTCGCATTGG
AAGCCTTGGGATACGAAAAAGGCCGCTACGACATCGTCAATCCGATGGATCATGTCAATGCCAGCCAATCGACCAACGAT
GCCTATCCCACGGGCTTCCGCCTTGCCGTGTATTACAGCATCGGCGAATTGCTCGACAAACTGACCGTCCTGAAAAACGC
CTTTACCGCCAAAGCCGAAGAATTTAAAGACGTTTTGAAAATGGGTCGCACCCAGCTTCAAGATGCCGTACCGATGACGG
CAGGACAAGAATTCCAATCTTTCCAAGTGTTGTTGGAAGAAGAAATCCTCAACCTCGACCGCACGCGCCAACTGCTTTTA
GAAGTCAATTTGGGCGCAACGGCAATCGGCACGGGCGTGAACACGCCCAAAGGCTACGCCGAACTGGTGGTCAAAAAACT
CTCCGAAGTCAGCGGCTTGCCTTGCAAACTGACCGAAAACCTGATTGAGGCGACCTCCGACTGCGGCGCATATGTGATGG
TACACGGCGCATTGAAGCGCACGGCGGTCAAACTCTCCAAAATCTGCAACGACTTGCGCCTGCTCTCTTCCGGCCCGCGT
GCCGGCTTGAAAGAAATCAACCTGCCCGAATTACAGGCCGGTTCTTCCATTATGCCTGCCAAAGTCAATCCCGTGATTCC
CGAAGTGGTCAACCAAGTCTGCTTCAAAGTCATCGGCAACGATACGACGATTACCTTCGCCGCCGAAGCCGGGCAACTGC
AATTAAACGTGATGGAGCCGGTCATCGCCCAATGTATGTTTGAAACCATTTCCCTCTTGGGCAATGCAGCAATCAACCTG
TCCGACAAATGCGTCAAAGGCATTACGGTCAACCGCGAAATATGCGAGCGTTACGTTTTCAACTCCATCGGGCTGGTTAC
TTATCTGAATCCGTATATCGGACACCACAACGGCGATTTGGTCGGCAAAATCTGCGCCCAAACCGGCAAAGGCGTGCGCG
AGGTTGTCTTGGAACGCGGGCTGTTGAGCGAAGAAGAACTCAACCGCATCCTCTCTCCTGAAAACCTGATGAACCCCCAT
CTGTAA

Upstream 100 bases:

>100_bases
TATAACACCCGAAAACAACGTTTCCCGTTTTCAGACGGCATACGATATGGACAATTTCATCCGGCCGGTATAGTGTTCAT
CCTGCGGCGGCATCTTTCCG

Downstream 100 bases:

>100_bases
CGTTGCAGACAAATGCCGTCTGAAGCAGGGATGCTGCTTCAGACGGCATTTTGTTATATCAATCAGCCGGATTATATGCC
GAACAGTACCTTGACTTCCC

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MAHFFNINYPTYKGKNMTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQFVRSMVMVKKATAQANGELG
AVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGTSVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTND
AYPTGFRLAVYYSIGELLDKLTVLKNAFTAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDRTRQLLL
EVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYVMVHGALKRTAVKLSKICNDLRLLSSGPR
AGLKEINLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINL
SDKCVKGITVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEELNRILSPENLMNPH
L

Sequences:

>Translated_481_residues
MAHFFNINYPTYKGKNMTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQFVRSMVMVKKATAQANGELG
AVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGTSVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTND
AYPTGFRLAVYYSIGELLDKLTVLKNAFTAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDRTRQLLL
EVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYVMVHGALKRTAVKLSKICNDLRLLSSGPR
AGLKEINLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINL
SDKCVKGITVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEELNRILSPENLMNPH
L
>Mature_480_residues
AHFFNINYPTYKGKNMTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQFVRSMVMVKKATAQANGELGA
VKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGTSVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDA
YPTGFRLAVYYSIGELLDKLTVLKNAFTAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDRTRQLLLE
VNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYVMVHGALKRTAVKLSKICNDLRLLSSGPRA
GLKEINLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLS
DKCVKGITVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEELNRILSPENLMNPHL

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=463, Percent_Identity=39.0928725701944, Blast_Score=328, Evalue=8e-90,
Organism=Escherichia coli, GI87082375, Length=461, Percent_Identity=72.885032537961, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=461, Percent_Identity=38.6117136659436, Blast_Score=331, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=41.3419913419913, Blast_Score=357, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=41.4110429447853, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6324993, Length=465, Percent_Identity=40.6451612903226, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24662684, Length=468, Percent_Identity=40.1709401709402, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=39.6963123644252, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=39.6963123644252, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=38.8768898488121, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=34.1252699784017, Blast_Score=289, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52705; Mature: 52574

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHFFNINYPTYKGKNMTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
CCEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGTSV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCCCCEE
NMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGELLDK
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHH
LTVLKNAFTAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDRTRQLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHE
EVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYVMVHGALKR
EECCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEHHHHHH
TAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
DTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLSDKCVKGITVNREICERYVF
CCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHH
NSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEELNRILSPENLMNPH
HHCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHCCCHHHCCCC
L
C
>Mature Secondary Structure 
AHFFNINYPTYKGKNMTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
CEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGTSV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCCCCEE
NMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGELLDK
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHH
LTVLKNAFTAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDRTRQLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHE
EVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYVMVHGALKR
EECCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEHHHHHH
TAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
DTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLSDKCVKGITVNREICERYVF
CCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHH
NSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEELNRILSPENLMNPH
HHCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHCCCHHHCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]