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The map label for this gene is gshA

Identifier: 161870119

GI number: 161870119

Start: 1148371

End: 1149540

Strand: Reverse

Name: gshA

Synonym: NMCC_1158

Alternate gene names: NA

Gene position: 1149540-1148371 (Counterclockwise)

Preceding gene: 161870128

Following gene: 161870118

Centisome position: 53.38

GC content: 53.68

Gene sequence:

>1170_bases
ATGAATTTGTTCCCCGGCGGCTTCAATAATCTGAATCCCAACTTTATCCCGCTGGCGGCGGTTGCCGCGCAAGATGCGGT
GCAACGCGCCTGTGAAACGGCGAAATCCGTATTGATTATTCCCGAAAACCACACGCGCAATATGTTTTACCTGCAAAACG
TTTACGCCCTCGGCGAGATTTTGTGTTCGGCAGGGTATGAAGTGCGCTTGGGCAGCCTGAATCCGGAAGTAACCGAACCG
ACCGAATTTGAAACCGCATTGGGCGACAAAATCCTGTTGGAACCTTTATTGCGTACCCGCGACCGCGTCCATCTTGCAGA
CGGCTTTTCGCCTTGCGTGGTTTTGTTGAACAACGATTTGTCCGCCGGTATTCCCGACATCCTCAAAGGCATCAGCCAAA
CCGTTTTGCCGCCGTTGCACGGCGGTTGGACGACGCGCCGCAAAACAAATCATTTCGGCGCGTACAACCAAGTTGCCGCC
GAATTTGCCAAGTTAATCGGCATCGACGAATGGCAAATTAACCCTTATTTTGAAAAAATCGGCGGTTTGGACTTCCAAGG
GCGTGAAGGCGAAGACGCGTTGGCGGAGGCGGTAGAACGTGTGCTGGCGAAAATTCAAGCCAAATACGACGAATCGGGCA
TTACCGACAAACCTTTCGTCATCGTCAAAGCCGATGCCGGCACTTACGGCATGGGCGTGATGAGCGTCAAATCCGCCGAC
GAAGTGCGCGGATTGAACCGTAAAAACCGCAATAAAATGGCGAAAGTCAAAGAAGGTTTGGAAGTCAGCGAAGTGATTGT
CCAAGAAGGGATTTATACTTATGAAACCTTAAACGGCGCGGTGTGCGAACCCGTCGTGTATATGATGGACCGCTTCGTCA
TCGGCGGCTTTTTCCGCGTACACGAAGGGCGCGGTGCGGACGAAAACCTCAACGCCGGCGGTATGGTGTTTGTTCCGCTG
TCCAACAGCATCCCCACCGGCAACGGCGACAACTCCCAAGAAGCTCCCGAAGCCTGCAAGCGCGTATTCGAACAATGGGA
CTCGCTGGGTATGCCGCGCTCTGAAAAAGACTGCGACGTGGACAACGAACACAACCGCCTCTACGTTTACGGCGTAATGG
CACGCCTGTCGCTTCTGGCGGCGTCCATCGAGTTGGAAGAAACGGCGTAA

Upstream 100 bases:

>100_bases
AATCGAGGCGTGGTTCCGCACGCAGTGGAGCGTACACCGCCCGCCGTTTTACGGTTCGGTCGATATACGCAATGCCGGTT
ACAAAATTTCGTCTATCGAT

Downstream 100 bases:

>100_bases
GACTGTTTTGAAATACAGATGCCGTCTGAAGCGGAAATCCGGTTCAGACGGCATTTCGGATATTTGGCGTGTGGGAACAT
TTGTTCCAGACGGCATCTCA

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPENHTRNMFYLQNVYALGEILCSAGYEVRLGSLNPEVTEP
TEFETALGDKILLEPLLRTRDRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQVAA
EFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITDKPFVIVKADAGTYGMGVMSVKSAD
EVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTYETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPL
SNSIPTGNGDNSQEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA

Sequences:

>Translated_389_residues
MNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPENHTRNMFYLQNVYALGEILCSAGYEVRLGSLNPEVTEP
TEFETALGDKILLEPLLRTRDRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQVAA
EFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITDKPFVIVKADAGTYGMGVMSVKSAD
EVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTYETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPL
SNSIPTGNGDNSQEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA
>Mature_389_residues
MNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPENHTRNMFYLQNVYALGEILCSAGYEVRLGSLNPEVTEP
TEFETALGDKILLEPLLRTRDRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQVAA
EFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITDKPFVIVKADAGTYGMGVMSVKSAD
EVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTYETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPL
SNSIPTGNGDNSQEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 42768; Mature: 42768

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPENHTRNMFYLQNVYALGEI
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEHHHHHHHHHHH
LCSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTRDRVHLADGFSPCVVLLNNDL
HHHCCCEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCEEECCCCCCEEEEECCCC
SAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQVAAEFAKLIGIDEWQINPYFEKI
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
GGLDFQGREGEDALAEAVERVLAKIQAKYDESGITDKPFVIVKADAGTYGMGVMSVKSAD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEECCHH
EVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTYETLNGAVCEPVVYMMDRFVIGGFFRV
HHHCCCCCHHHHHHHHHHCCHHHHHHHHCCCCEEHHCCCHHHHHHHHHHHHHHHCCEEEE
HEGRGADENLNAGGMVFVPLSNSIPTGNGDNSQEAPEACKRVFEQWDSLGMPRSEKDCDV
ECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DNEHNRLYVYGVMARLSLLAASIELEETA
CCCCCEEEEEEHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPENHTRNMFYLQNVYALGEI
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEHHHHHHHHHHH
LCSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTRDRVHLADGFSPCVVLLNNDL
HHHCCCEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCEEECCCCCCEEEEECCCC
SAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQVAAEFAKLIGIDEWQINPYFEKI
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
GGLDFQGREGEDALAEAVERVLAKIQAKYDESGITDKPFVIVKADAGTYGMGVMSVKSAD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEECCHH
EVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTYETLNGAVCEPVVYMMDRFVIGGFFRV
HHHCCCCCHHHHHHHHHHCCHHHHHHHHCCCCEEHHCCCHHHHHHHHHHHHHHHCCEEEE
HEGRGADENLNAGGMVFVPLSNSIPTGNGDNSQEAPEACKRVFEQWDSLGMPRSEKDCDV
ECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DNEHNRLYVYGVMARLSLLAASIELEETA
CCCCCEEEEEEHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA