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The map label for this gene is lipA [H]

Identifier: 161870059

GI number: 161870059

Start: 1082546

End: 1083514

Strand: Reverse

Name: lipA [H]

Synonym: NMCC_1095

Alternate gene names: 161870059

Gene position: 1083514-1082546 (Counterclockwise)

Preceding gene: 161870060

Following gene: 161870053

Centisome position: 50.32

GC content: 54.49

Gene sequence:

>969_bases
ATGAGTGAAATCAAAACCGACGACCCCAAACGCGGCATCAAACTCAGAGGCGCGGACAAAACCGCGCGCATCCCCATCAA
AGTCGTCCCCCTTCAGGAAAAACTGAAAAAGCCCGAATGGATACGCGCCAAACTCCCATCGCGAAAATTCTTTGAAATCA
AAGACATTTTGCGCGAACAAAAGATGCACACCGTTTGCGAGGAAGCCTCCTGCCCCAACATCGGCGAATGCTTCAGCAAA
GGCACGGCGACCTTCATGATTATGGGTGACATCTGCACCCGCCGCTGCCCGTTCTGCGACGTGGGACACGGTCGGCCCAA
TATGCTCGACCCCGACGAACCGAGAAACCTCGCCGAATCCGTCAAAGCCATGAACCTGCGTTACGTCGTCATCACCTCCG
TCGACCGCGACGACCTGCGCGACGGCGGCGCACAGCATTTCGCCGACTGCATCAAAGCCATCCGCGAAACCAGCCCGAAC
ACCAAAATCGAAATCCTCGTCCCCGACTTCCGAGGACGCTTGGACATCGCACTCAAAATCCTTGCCGAAACCCCGCCCGA
CGTGATGAACCACAACCTAGAAACCCATCCGAGCCTGTACAGAAAAGCCCGTCCCGGTGCCAATTATCAACATTCTTTAG
ACTTATTAAAACGTTATAAAGAAATGATGCCGCACATCCCGACCAAATCCGGCATCATGGTCGGCTTGGGCGAAACAGAC
GAAGACGTGCGTGAAATTATGCGCGATATGCGGGCGCACAATATCGAAATGATTACCATCGGACAGTACCTCCAGCCTTC
AGACGGACACCTGCCCGTCCTGCGCTACGTTACGCCCGAGCAGTTCAAAATCTTTGAAAAAGAAGCATACGAACTGGGCT
TCAGCAATGCCGCCATCGGCGCGATGGTACGTTCCAGCTACCACGCCGACGAACAGGCTGCCGAAGCCTTACGCGAATGT
GAATTCTGA

Upstream 100 bases:

>100_bases
CAAATCGCGGATTTTGTCCAACCCTGCCCCACGCCGGACGAAGTCGCCGCCAAACTCACCGCACACCTTGAAACACAATT
CACACCGAAAGCAGACAACA

Downstream 100 bases:

>100_bases
CGCTATTTGAGACTGAACTACGCTATTTGCGACTTTATAACGCTAAGTTCGACTGGGCAAATATGGATCAACAACCAGTT
ATTGAAATTGCCGAATTCCC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAKLPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSK
GTATFMIMGDICTRRCPFCDVGHGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKRYKEMMPHIPTKSGIMVGLGETD
EDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRYVTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALREC
EF

Sequences:

>Translated_322_residues
MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAKLPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSK
GTATFMIMGDICTRRCPFCDVGHGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKRYKEMMPHIPTKSGIMVGLGETD
EDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRYVTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALREC
EF
>Mature_321_residues
SEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAKLPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKG
TATFMIMGDICTRRCPFCDVGHGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPNT
KIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKRYKEMMPHIPTKSGIMVGLGETDE
DVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRYVTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRECE
F

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=287, Percent_Identity=47.0383275261324, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI37577164, Length=249, Percent_Identity=45.7831325301205, Blast_Score=223, Evalue=2e-58,
Organism=Escherichia coli, GI1786846, Length=308, Percent_Identity=63.3116883116883, Blast_Score=408, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32564533, Length=293, Percent_Identity=42.6621160409556, Blast_Score=228, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=292, Percent_Identity=45.2054794520548, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=45.8333333333333, Blast_Score=262, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36668; Mature: 36537

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAKLPSRKFFEIKDILREQ
CCCCCCCCCCCCCEEECCCCCCCCCEEEECHHHHCCCCCHHHHCCCCCHHHHHHHHHHHH
KMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVGHGRPNMLDPDEPRNLAES
HHHHHHHHCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
VKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPNTKIEILVPDFRGRLDIALKI
HHHCCEEEEEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
LAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKRYKEMMPHIPTKSGIMVGLGETD
HHCCCHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCH
EDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRYVTPEQFKIFEKEAYELGFSNAAIG
HHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHH
AMVRSSYHADEQAAEALRECEF
HHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAKLPSRKFFEIKDILREQ
CCCCCCCCCCCCEEECCCCCCCCCEEEECHHHHCCCCCHHHHCCCCCHHHHHHHHHHHH
KMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVGHGRPNMLDPDEPRNLAES
HHHHHHHHCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
VKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPNTKIEILVPDFRGRLDIALKI
HHHCCEEEEEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
LAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKRYKEMMPHIPTKSGIMVGLGETD
HHCCCHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCH
EDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRYVTPEQFKIFEKEAYELGFSNAAIG
HHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHH
AMVRSSYHADEQAAEALRECEF
HHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA