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The map label for this gene is hda [H]

Identifier: 161869962

GI number: 161869962

Start: 997438

End: 998169

Strand: Reverse

Name: hda [H]

Synonym: NMCC_0995

Alternate gene names: 161869962

Gene position: 998169-997438 (Counterclockwise)

Preceding gene: 161869963

Following gene: 161869961

Centisome position: 46.35

GC content: 54.37

Gene sequence:

>732_bases
TTGGAAAGGCGCGCGAAAACAACGTACAATAAACCCGTTTACCACCCCTCGGAATGCCGAACCGTGAACCAGCTTATTTT
CGACTTTGCCGCACACGACTATCCGAGTTTCGACAAATTCCTCGGCACGGAAAACGCGGAACTGGTCTATGTCCTCCGAC
ACAAACACGGACAGTTTATCTATGTCTGGGGTGAAGAAGGCGCGGGCAAAAGCCATCTTTTGCAGGCGTGGGTCGCACAG
GCGCTCGAAGCCGGCAAAAACGCCGCCTATATCGATGCCGCCTCCATGCCGCTGACCGATGCCGCGTTCGAGGCGGAATA
CCTCGCCGTCGATCAAGTCGAAAAACTGGGCAACGAAGAACAAGCCCTGCTTTTTTCCATCTTCAACCGCTTCCGCAACA
GCGGCAAAGGCTTTTTGCTGCTCGGTTCGGAATACACACCCCAGCAGCTCGTCATCCGCGAAGATTTGCGGACACGTATG
GCGTACTGCCTCGTTTACGAAGTCAAACCCTTAACCGACCAAGAAAAAATCGACGCGCTCGCCAGTATGGCGGCGGCACG
CCAAGTAACCGTCGATTCCGAAATTTTCGAATACCTGCTCAAACACTGGCGGCGCGATATGGACAGCCTGATGATGATGC
TCGACACGCTGGACAACTACGCCGTCACGATGGGCAAACGCATCACCCTGCCGCTTTTGCGCCAGCTTTTGAAACAACAG
GAAACCCAATGA

Upstream 100 bases:

>100_bases
GCACGCTCAACGCGTTCCACAATATGCGCCTCGATGTCGGCAACGTTGCCTGCGGTTGAACGATGCCGTCTGAAGCCGTT
TTCAGACGGCATCGTTCAAA

Downstream 100 bases:

>100_bases
AAAACCTCGCCATCTTCGACCTCGACAACACCCTCATCAACACCGATTCCGACCACGCCTGGCCGCAATACCTCATCAAA
AAAGGCTTGGTTGACGCTGC

Product: DnaA regulatory inactivator Hda

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MERRAKTTYNKPVYHPSECRTVNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGEEGAGKSHLLQAWVAQ
ALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQALLFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRM
AYCLVYEVKPLTDQEKIDALASMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQQ
ETQ

Sequences:

>Translated_243_residues
MERRAKTTYNKPVYHPSECRTVNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGEEGAGKSHLLQAWVAQ
ALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQALLFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRM
AYCLVYEVKPLTDQEKIDALASMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQQ
ETQ
>Mature_243_residues
MERRAKTTYNKPVYHPSECRTVNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGEEGAGKSHLLQAWVAQ
ALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQALLFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRM
AYCLVYEVKPLTDQEKIDALASMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQQ
ETQ

Specific function: Mediates the interactions of dnaA with the beta subunit sliding clamp, promoting hydrolysis of dnaA-bound ATP. It is essential for the control of initiation of DNA replication by inhibiting reinitiation of replication [H]

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family. HdA subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510964, Length=230, Percent_Identity=31.304347826087, Blast_Score=118, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020591
- InterPro:   IPR017788
- InterPro:   IPR013317
- InterPro:   IPR022864 [H]

Pfam domain/function: PF00308 Bac_DnaA [H]

EC number: NA

Molecular weight: Translated: 27945; Mature: 27945

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00675 SIGMA54_INTERACT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERRAKTTYNKPVYHPSECRTVNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEEECCCCEE
YVWGEEGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEE
EEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHH
QALLFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDAL
HHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHH
ASMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQQ
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECHHHHHHHHHHH
ETQ
CCC
>Mature Secondary Structure
MERRAKTTYNKPVYHPSECRTVNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEEECCCCEE
YVWGEEGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEE
EEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHH
QALLFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDAL
HHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHH
ASMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQQ
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECHHHHHHHHHHH
ETQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA