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The map label for this gene is leuA [H]

Identifier: 161869955

GI number: 161869955

Start: 989243

End: 991036

Strand: Direct

Name: leuA [H]

Synonym: NMCC_0988

Alternate gene names: 161869955

Gene position: 989243-991036 (Clockwise)

Preceding gene: 161869952

Following gene: 161869956

Centisome position: 45.94

GC content: 55.69

Gene sequence:

>1794_bases
ATGCAATTAGATATAGACCGCTTGGTTGCTTATTTCGGCGGCGTGAACGCGCTTGCCGAAGCGTTGAAACAGCACGATCC
CGAAAATGCCGCGACGACCGCCGCCATCTATAAATGGCGCACGCGCGGCTCGCTGCCTCTGGCGCAACTGCAAAAGCTGA
CCGCGTTGGCGGAAGCGCAAGGCAGGCCGCTGGATTTGAATGCTTTTTTACAAAAAAACGAATCTCTGGAGAGAACAGAA
ATGACACAGGCCAACCGCGTTATCATTTTCGACACCACCCTGCGCGACGGCGAGCAGTCGCCCGGCGCCGCTATGACCAA
AGAGGAAAAAATCCGCGTCGCCCGCCAGCTGGAAAAATTGGGTGTGGACATCATCGAAGCGGGTTTTGCCGCTGCCAGCC
CGGGCGATTTCGAGGCGGTCAATGCGATTGCGAAAACCATTACCAAATCAACGGTCTGTTCATTGTCCCGCGCCATCGAG
CGGGACATCCGTCAGGCGGGTGAGGCCGTTGCGCCCGCGCCGAAAAAACGCATCCACACCTTCATCGCCACCAGCCCCAT
CCATATGGAGTACAAATTGAAGATGAAGCCGAAGCAGGTGATTGAGGCGGCGGTCAAAGCGGTGAAAATCGCTCGTGAAT
ACACCGACGATGTGGAATTTTCCTGCGAAGACGCGTTGCGTTCGGAAATCGATTTCCTTGCCGAAATCTGCGGCGCGGTG
ATTGAAGCGGGCGCGACCACCATCAATATTCCCGATACCGTCGGCTATTCCATCCCGTACAAAACCGAAGAATTTTTCCG
CGAACTGATTGCCAAAACGCCCAACGGCGGCAAAGTCGTTTGGTCGGCACACTGCCACAACGATTTGGGCTTGGCGGTTG
CCAATTCGCTTGCCGCATTAAAAGGCGGTGCGCGTCAGGTGGAATGTACTGTCAACGGCTTGGGCGAACGTGCAGGCAAT
GCTTCGGTTGAAGAAATCGTGATGGCGTTGAAAGTGCGCCACGACTTGTTCGGCTTGGAAACCGGCATCGATACCACGCA
AATCGTGCCTTCGTCCAAACTGGTGTCCACCATTACGGGCTATCCCGTGCAGCCCAACAAAGCCATTGTCGGTGCCAATG
CCTTTTCGCATGAATCGGGCATCCATCAGGACGGGGTGCTGAAACACCGCGAAACTTACGAGATTATGTCCGCCGAATCG
GTCGGCTGGGCAACAAACCGTTTGAGCTTGGGCAAATTGTCCGGCCGCAACGCCTTCAAAACCAAGCTGGCGGATTTGGG
CATCGAGTTGGAAAGCGAAGAGGCACTGAACGCCGCGTTTGCCCGCTTCAAAGAACTCGCCGACAAAAAACGCGAAATCT
TCGATGAAGACCTGCACGCACTGGTATCCGACGAAATGGGCAGCATGAATGCCGAGAGCTACAAATTCATCTCCCAAAAA
ATCAGCACCGAAACCGGCGAAGAGCCGCGCGCCGACATCGTGTTCAGCATCAAAGGCGAAGAAAAACGCGCTTCCGCCAC
AGGCTCCGGCCCCGTGGATGCGATTTTCAAAGCGATTGAAAGCGTGGCGCAAAGCGGCGCGGCTTTGCAGATTTATTCCG
TCAACGCCGTCACGCAAGGTACGGAAAGCCAGGGCGAAACCAGCGTCCGTCTGGCGCGTGGCAACCGCGTTGTCAACGGT
CAGGGTGCGGATACCGACGTTTTGGTCGCCACTGCCAAAGCCTACCTCTCTGCCTTAAGCAAACTGGAATTTGGTTCTGC
CAAACCGAAAGCGCAAGGCAGCGGTACGATTTGA

Upstream 100 bases:

>100_bases
TATTGAGATTTTTCAAATTTATATTGCCGTTTTGTCCAAAATGCGTATAATCCTGTCCATATTTCTGCTGTAGGCTGATT
TATTTTAGACAAGGACTACC

Downstream 100 bases:

>100_bases
GCGTGAAAACAGACGATGCCGTCTGAAGCATAAAAAGGCTTCAGACGGCATTGCGGCGATATAGTGGATTAAATTTAAAC
CAGTACGGCGTTGCCTCGCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGSLPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTE
MTQANRVIIFDTTLRDGEQSPGAAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDVEFSCEDALRSEIDFLAEICGAV
IEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNGGKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGN
ASVEEIVMALKVRHDLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRETYEIMSAES
VGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKKREIFDEDLHALVSDEMGSMNAESYKFISQK
ISTETGEEPRADIVFSIKGEEKRASATGSGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNG
QGADTDVLVATAKAYLSALSKLEFGSAKPKAQGSGTI

Sequences:

>Translated_597_residues
MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGSLPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTE
MTQANRVIIFDTTLRDGEQSPGAAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDVEFSCEDALRSEIDFLAEICGAV
IEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNGGKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGN
ASVEEIVMALKVRHDLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRETYEIMSAES
VGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKKREIFDEDLHALVSDEMGSMNAESYKFISQK
ISTETGEEPRADIVFSIKGEEKRASATGSGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNG
QGADTDVLVATAKAYLSALSKLEFGSAKPKAQGSGTI
>Mature_597_residues
MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGSLPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTE
MTQANRVIIFDTTLRDGEQSPGAAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDVEFSCEDALRSEIDFLAEICGAV
IEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNGGKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGN
ASVEEIVMALKVRHDLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRETYEIMSAES
VGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKKREIFDEDLHALVSDEMGSMNAESYKFISQK
ISTETGEEPRADIVFSIKGEEKRASATGSGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNG
QGADTDVLVATAKAYLSALSKLEFGSAKPKAQGSGTI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=501, Percent_Identity=46.9061876247505, Blast_Score=443, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320071, Length=380, Percent_Identity=29.2105263157895, Blast_Score=143, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6320019, Length=393, Percent_Identity=28.7531806615776, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324225, Length=591, Percent_Identity=25.8883248730964, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324682, Length=560, Percent_Identity=25.7142857142857, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 64201; Mature: 64201

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGSLPLAQLQKLTALAEAQ
CCCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHCC
GRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPGAAMTKEEKIRVARQLEKL
CCCCCHHHHHHCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIERDIRQAGEAVAPAPKKRIHT
CHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
FIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDVEFSCEDALRSEIDFLAEICGAV
HHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
IEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNGGKVVWSAHCHNDLGLAVANSLAAL
HHCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
KGGARQVECTVNGLGERAGNASVEEIVMALKVRHDLFGLETGIDTTQIVPSSKLVSTITG
CCCCEEEEEEECCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHCC
YPVQPNKAIVGANAFSHESGIHQDGVLKHRETYEIMSAESVGWATNRLSLGKLSGRNAFK
CCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHH
TKLADLGIELESEEALNAAFARFKELADKKREIFDEDLHALVSDEMGSMNAESYKFISQK
HHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ISTETGEEPRADIVFSIKGEEKRASATGSGPVDAIFKAIESVAQSGAALQIYSVNAVTQG
HCCCCCCCCCCCEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCEECC
TESQGETSVRLARGNRVVNGQGADTDVLVATAKAYLSALSKLEFGSAKPKAQGSGTI
CCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGSLPLAQLQKLTALAEAQ
CCCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHCC
GRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPGAAMTKEEKIRVARQLEKL
CCCCCHHHHHHCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIERDIRQAGEAVAPAPKKRIHT
CHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
FIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDVEFSCEDALRSEIDFLAEICGAV
HHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
IEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNGGKVVWSAHCHNDLGLAVANSLAAL
HHCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
KGGARQVECTVNGLGERAGNASVEEIVMALKVRHDLFGLETGIDTTQIVPSSKLVSTITG
CCCCEEEEEEECCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHCC
YPVQPNKAIVGANAFSHESGIHQDGVLKHRETYEIMSAESVGWATNRLSLGKLSGRNAFK
CCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHH
TKLADLGIELESEEALNAAFARFKELADKKREIFDEDLHALVSDEMGSMNAESYKFISQK
HHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ISTETGEEPRADIVFSIKGEEKRASATGSGPVDAIFKAIESVAQSGAALQIYSVNAVTQG
HCCCCCCCCCCCEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCEECC
TESQGETSVRLARGNRVVNGQGADTDVLVATAKAYLSALSKLEFGSAKPKAQGSGTI
CCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA