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The map label for this gene is proB [H]

Identifier: 161869954

GI number: 161869954

Start: 987889

End: 988752

Strand: Reverse

Name: proB [H]

Synonym: NMCC_0987

Alternate gene names: 161869954

Gene position: 988752-987889 (Counterclockwise)

Preceding gene: 161869957

Following gene: 161869953

Centisome position: 45.92

GC content: 56.71

Gene sequence:

>864_bases
ATGGAAGAATATACGGCAAACCTGTCTTCAGACGGCATCGTGTCCGCGCAAATCCTGCTCAGCCGCGCCGACTTTGCCGA
CAAACGCCGCTACCAAAATGCCGGCGGCGCACTTTCCGTGCTGCTGCAACGCCGCGCCGTCCCCATCATCAATGAAAACG
ATACGGTTTCGGTTGAGGAATTGAAAATCGGCGACAACGACACATTGAGTGCGCAAGTGGCGGCGATGATACAGGCAGAC
CTCTTGGTGCTGCTGACCGACATAGACGGTCTTTACACGGGCAACCCGAACAGCAATCCCGATGCCGTACGGCTGGACAA
AATCGAACACATCAACCATGAAATCATCGAAATGGCGGGCGGATCGGGTTCGGCAAACGGCACGGGCGGTATGCTGACCA
AAATCAAAGCGGCAACCATCGCCGCCGAATCCGGCGTACCGGTGTATATCTGTTCCTCGCTCAAACCCGATGCACTTGCC
GAAGCTGCCGAACATCAGGCGGACGGCTCGTTTTTCGTCCCCCGTGCCAAAGGTTTGCGGACGCAGAAGCAATGGCTGGC
GTTCTATTCCGAAAGCCGGGGCAGCGTTTATGTGGACGAAGGTGCGGAACACGCTTTGTCCGAACAGGGGAAAAGCCTGC
TGATGTCGGGCATTGCCGGAATCGAAGGGCATTTTTCCCGTATGGACACCGTAACCGTGTACAGCAAGGCAACCAAACAG
CCCCTGGGCAAAGGGCGCGTCCTGTTCGGTTCTGCCGCCGCCGAAGACCTGCTCAAATCGCGTAAGGCGAAAGGCGTGTT
CATCCATCGGGACGACTGGATTTCCATCACGCCCGAAATACGCCTGCTTCTGACCGAATTTTAG

Upstream 100 bases:

>100_bases
CGGCGCGGTTGCGGCAGGGTTCGGTGCGCTGGGTTTCAAAAAACGTCCGGTCAAAATCGCCGACAAACAGGCTTCCGCCG
CCGTCGGGCAGGGGCTGCTG

Downstream 100 bases:

>100_bases
AAAAGGAAACCCATGTCAAACACACAAAAACAGCTTGCCCTTGCCAAAGCGGCAAAAAAATCCGTCAACACGGCGGATAC
GGAAGAAAAAAACCGCGCGC

Product: glutamate 5-kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MEEYTANLSSDGIVSAQILLSRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQAD
LLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIKAATIAAESGVPVYICSSLKPDALA
EAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRGSVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQ
PLGKGRVLFGSAAAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF

Sequences:

>Translated_287_residues
MEEYTANLSSDGIVSAQILLSRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQAD
LLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIKAATIAAESGVPVYICSSLKPDALA
EAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRGSVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQ
PLGKGRVLFGSAAAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF
>Mature_287_residues
MEEYTANLSSDGIVSAQILLSRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQAD
LLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIKAATIAAESGVPVYICSSLKPDALA
EAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRGSVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQ
PLGKGRVLFGSAAAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=187, Percent_Identity=32.620320855615, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI62912457, Length=185, Percent_Identity=32.4324324324324, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1786437, Length=274, Percent_Identity=37.956204379562, Blast_Score=171, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17569969, Length=176, Percent_Identity=33.5227272727273, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17569967, Length=176, Percent_Identity=33.5227272727273, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320506, Length=258, Percent_Identity=36.046511627907, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321822, Length=181, Percent_Identity=41.4364640883978, Blast_Score=135, Evalue=7e-33,
Organism=Drosophila melanogaster, GI21357643, Length=186, Percent_Identity=35.4838709677419, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 30851; Mature: 30851

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEYTANLSSDGIVSAQILLSRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEE
CCCCCCCCCCCCEEEHHHHHHHHCHHHHHHHCCCCHHHHHHHHHCCCCEECCCCCEEEEE
LKIGDNDTLSAQVAAMIQADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAG
EECCCCCCHHHHHHHHHHHHHEEEEECCCCEEECCCCCCCCEEEEHHHHHCCHHHHEECC
GSGSANGTGGMLTKIKAATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLR
CCCCCCCCCCHHEEEEEHEEEECCCCCEEEECCCCCHHHHHHHHHCCCCCEECCCCCCCC
TQKQWLAFYSESRGSVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQ
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEHHHHCC
PLGKGRVLFGSAAAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF
CCCCCEEEECCHHHHHHHHHHCCCEEEEECCCCEEECCCEEEEEECC
>Mature Secondary Structure
MEEYTANLSSDGIVSAQILLSRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEE
CCCCCCCCCCCCEEEHHHHHHHHCHHHHHHHCCCCHHHHHHHHHCCCCEECCCCCEEEEE
LKIGDNDTLSAQVAAMIQADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAG
EECCCCCCHHHHHHHHHHHHHEEEEECCCCEEECCCCCCCCEEEEHHHHHCCHHHHEECC
GSGSANGTGGMLTKIKAATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLR
CCCCCCCCCCHHEEEEEHEEEECCCCCEEEECCCCCHHHHHHHHHCCCCCEECCCCCCCC
TQKQWLAFYSESRGSVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQ
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEHHHHCC
PLGKGRVLFGSAAAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF
CCCCCEEEECCHHHHHHHHHHCCCEEEEECCCCEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]