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The map label for this gene is sbp [H]

Identifier: 161869929

GI number: 161869929

Start: 961537

End: 962592

Strand: Reverse

Name: sbp [H]

Synonym: NMCC_0961

Alternate gene names: 161869929

Gene position: 962592-961537 (Counterclockwise)

Preceding gene: 161869930

Following gene: 161869928

Centisome position: 44.7

GC content: 53.79

Gene sequence:

>1056_bases
ATGAAAACCTACGCACCGGCACTCTATACCGCAGCCCTGCTCACCGCCTGCAGCCCCACAGCCGATTCAAACCATCCGTC
CGGACAAAATGCCCCGGCCAATACCGAATCCGACGGAAAAAACATTACCCTGCTCAACGCCTCATACGATTTGGCACGGG
ATTTTTACAAAGAATACAACCCCTTATTTATCAAAACATACCAATCCGAACACCCCGGCACATCCGTCAGCATCCAACAG
TCCCACGGCGGCTCCAGCAAACAGGCATTATCCGTAGCCAACGGCCTTCAAGCCGATGTCGTAACCATGAACCAATCCTC
CGACATCGACCTGCTCGAAAAAAAAGGACTGGTAGAAAAAGGCTGGCAACAAGCCCTCCCCGACCACGCCGCGCCCTACA
CCAGCACTATGGTTTTCCTTGTCCGAAAAAACAACCCCAAACAGATCCGCGATTGGAACGACCTTGCCAAAGACGGCGTT
AACATCGTCATCGCCAATCCCAAAACCTCGGGCAACGGACGCTACGCCTTCCTCGGCGCATACGGTTACGGTCTGAAAAC
CACCAACGGCAACGAACAGGAAGCCCAAAAACTCGTCGCATCCATCCTCAAAAACACCCCCGTTTTTGAAAACGGCGGAC
GCGCCGCCACCACCACCTTCACACAACGCAACATCGGCGACGTACTCATCACTTTTGAAAACGAAGCCAACTACGTCAGC
AAAAAACTGACCCAAGGACAGTTTGAAATCGTCTATCCGAGCTACACCATTTCCGCCGAAAGCCCCGTCGCCGTCGTCAA
CAGCATCGTCGCGAAAAAAGGCACGCAAAAAACCGCCCGCGCCTATCTCGAATACCTTTGGAGCGAACCGGCGCAAGAAC
TCGCCGCCTCACTCTACCTGCGCCCCCGCAACCCCGAAGTATTGGCAAGACACAAAGCCGACTTCCCAGACTTAGACACA
TTCTCTCCCGAAGAAAAATTCGGCGGATGGGACAACATCATGAAAACCTACTTCGCCGACGGCGGCATATTCGACCGCCT
GACCGCACAAAAATGA

Upstream 100 bases:

>100_bases
CCTTATAACCAAAACTGATAATCTTCAAATCAAACATTATTTCCACAAGCGCAGACAGCCGTATAAAGTTGCCCCATCTA
CCAACCGACAGGACAAGCAT

Downstream 100 bases:

>100_bases
CCGCTATGCCGTCTGAACCGCCTTTCAGACGGCATTTCACACATCAAAACAGAAAAACCGCGCCCAAAATCATCATCACA
AGACACAACGGCAAACCGGA

Product: sulfate ABC transporter, periplasmic sulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKTYAPALYTAALLTACSPTADSNHPSGQNAPANTESDGKNITLLNASYDLARDFYKEYNPLFIKTYQSEHPGTSVSIQQ
SHGGSSKQALSVANGLQADVVTMNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATTTFTQRNIGDVLITFENEANYVS
KKLTQGQFEIVYPSYTISAESPVAVVNSIVAKKGTQKTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDT
FSPEEKFGGWDNIMKTYFADGGIFDRLTAQK

Sequences:

>Translated_351_residues
MKTYAPALYTAALLTACSPTADSNHPSGQNAPANTESDGKNITLLNASYDLARDFYKEYNPLFIKTYQSEHPGTSVSIQQ
SHGGSSKQALSVANGLQADVVTMNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATTTFTQRNIGDVLITFENEANYVS
KKLTQGQFEIVYPSYTISAESPVAVVNSIVAKKGTQKTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDT
FSPEEKFGGWDNIMKTYFADGGIFDRLTAQK
>Mature_351_residues
MKTYAPALYTAALLTACSPTADSNHPSGQNAPANTESDGKNITLLNASYDLARDFYKEYNPLFIKTYQSEHPGTSVSIQQ
SHGGSSKQALSVANGLQADVVTMNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATTTFTQRNIGDVLITFENEANYVS
KKLTQGQFEIVYPSYTISAESPVAVVNSIVAKKGTQKTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDT
FSPEEKFGGWDNIMKTYFADGGIFDRLTAQK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=318, Percent_Identity=48.4276729559748, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1788765, Length=307, Percent_Identity=46.9055374592834, Blast_Score=298, Evalue=5e-82,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38515; Mature: 38515

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYAPALYTAALLTACSPTADSNHPSGQNAPANTESDGKNITLLNASYDLARDFYKEYN
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCC
PLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVTMNQSSDIDLLEKKGLVEK
CEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCHHH
GWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGVNIVIANPKTSGNGRYAFLGA
HHHHHCCCCCCCCCEEEEEEEECCCCCHHHCHHHHHHCCCEEEEECCCCCCCCCEEEEEE
YGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATTTFTQRNIGDVLITFENEANYVS
ECCCEECCCCCHHHHHHHHHHHHHCCCCEECCCCEEEEEEECCCCCCEEEEEECCCHHHH
KKLTQGQFEIVYPSYTISAESPVAVVNSIVAKKGTQKTARAYLEYLWSEPAQELAASLYL
HHHCCCCEEEEECCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCC
RPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIMKTYFADGGIFDRLTAQK
CCCCCHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCCHHHHCCCC
>Mature Secondary Structure
MKTYAPALYTAALLTACSPTADSNHPSGQNAPANTESDGKNITLLNASYDLARDFYKEYN
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCC
PLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVTMNQSSDIDLLEKKGLVEK
CEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCHHH
GWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGVNIVIANPKTSGNGRYAFLGA
HHHHHCCCCCCCCCEEEEEEEECCCCCHHHCHHHHHHCCCEEEEECCCCCCCCCEEEEEE
YGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATTTFTQRNIGDVLITFENEANYVS
ECCCEECCCCCHHHHHHHHHHHHHCCCCEECCCCEEEEEEECCCCCCEEEEEECCCHHHH
KKLTQGQFEIVYPSYTISAESPVAVVNSIVAKKGTQKTARAYLEYLWSEPAQELAASLYL
HHHCCCCEEEEECCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCC
RPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIMKTYFADGGIFDRLTAQK
CCCCCHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]