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The map label for this gene is pmbA [H]

Identifier: 161869774

GI number: 161869774

Start: 808274

End: 809605

Strand: Reverse

Name: pmbA [H]

Synonym: NMCC_0797

Alternate gene names: 161869774

Gene position: 809605-808274 (Counterclockwise)

Preceding gene: 161869781

Following gene: 161869772

Centisome position: 37.6

GC content: 60.89

Gene sequence:

>1332_bases
ATGCTGTTCAACCACACCGCTTCCGAACTTCTCGACCTCTGCCGCCGCACGCTCGACTTGGCAAAAGCAGCGGGCGCGAC
CGCCGCCGAAGCCGATTTCAGCGAATCCATCGGACAAAGCGTCAGCGTGCGGCTGGGCGAAATCGAACAAATCGAGTTCC
AGCAGGACAAGTCGCTGGACATTACCGTTTACGTCGGCAAACGCAAAGGCCGCGCTGGCACCGCCGATTTCTCCGAACAA
GCCCTGCGCGACACCGTCCGCGCCGCCGCCGACATCGCCCGCCACACCGCCGAAGACGGTTGCGCCGGACTTGCCGACCC
CGAGTTGATGGCGCAACACATCGGCGACCCCGACCTTTACCACGAATGGGATTTGGATACGGAAGCCGCCGTCGGCTTTG
CAAAACAATGCGAACAAGCCGCCCTGAACGAGGATGAGCGCATCGAAAACTCCGAAGGCGCGGCGGTGCAAACCGGCCAT
TACCAATACGTTTACGGCAACACCCACGGTTTTGCCGCACACCGGCAAGGCACGCACCACAGCATTTCCTGCAGCGTCGT
TGCCGCCGACGAAAACGGGATGCAGCGCGACTACTGGTACGATTCCGCCTGCCGCCATCCGGATATGGACAGCCCCGAAA
CCATCGGTCAAACCGCCGCCCGCCGCACTTTGCGCCGACTCGGCAGCCGCAGCATCCCGACAGGCAGCTACCCCGTCCTC
TTCGATACCACCGTTTCGGGCGGTCTTATCGGACACCTCGTCGGCGCACTCTCCGGCGGCGCGCTCTACCGCCAAAGCAG
TTTCCTGATCGACAGCATCGGCAAAAAAGTCCTGCCCGATTTCCTCAACCTGCGCGAAGAACCGCACATCCCCCGCTCTT
TCCGCAGCAGCTATTTTGATGCGGAAGGCGTTGCCACCGCGCCGCGCTTCGTGATTCAAAACGGCATTGTCGAAGGCTAT
TTCCTCAGCAGTTACAGCGCGCGCAAACTCGGTATGCAGACCACGGGCAACGCCGGCGGCGCGCACAACCTGTATTTGAA
CCATACGCACGAAACGCAATCCGACCTGCTGAAAGAAATGGGGACGGGATTGTTGGTAACCGAACTGATGGGACAGGGCG
CGAACACCATTACCGGTGACTACTCGCGCGGCGCGGCGGGTTTTTGGGTGGAAAACGGCGTGATTGCCTACCCCGTCCAC
GAGATTACCGTAGCCGGACGCTTGCAGGATATGTACCGCGACATTGTCGGCGTGGCGGATGACGCTTTGCGGCGTTCGTC
CAACAAAATCGGCTCGATTCTGATTGCGGGGATGACGGTTGCCGGAAGCTGA

Upstream 100 bases:

>100_bases
CAGACGGCATTTTGCACCGTCCGCCCTGCAAACGGCACGCTTTTGCCCCTGCCGCCCGAAACGGTTAAAATGCCGCAACG
CTCCACCAAGAAAGATTTCT

Downstream 100 bases:

>100_bases
ACGCCCTGCCGATGCCGTCTGAAGACGAAGGCAGCACGTTTTGCCCCGTGCCTGCCCGTTTGCCGTTTTATGCCGCACCG
CCTTTTGCCGACAACATTGC

Product: pmbA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVRLGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQ
ALRDTVRAAADIARHTAEDGCAGLADPELMAQHIGDPDLYHEWDLDTEAAVGFAKQCEQAALNEDERIENSEGAAVQTGH
YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQTAARRTLRRLGSRSIPTGSYPVL
FDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKKVLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGY
FLSSYSARKLGMQTTGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVENGVIAYPVH
EITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS

Sequences:

>Translated_443_residues
MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVRLGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQ
ALRDTVRAAADIARHTAEDGCAGLADPELMAQHIGDPDLYHEWDLDTEAAVGFAKQCEQAALNEDERIENSEGAAVQTGH
YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQTAARRTLRRLGSRSIPTGSYPVL
FDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKKVLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGY
FLSSYSARKLGMQTTGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVENGVIAYPVH
EITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS
>Mature_443_residues
MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVRLGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQ
ALRDTVRAAADIARHTAEDGCAGLADPELMAQHIGDPDLYHEWDLDTEAAVGFAKQCEQAALNEDERIENSEGAAVQTGH
YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQTAARRTLRRLGSRSIPTGSYPVL
FDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKKVLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGY
FLSSYSARKLGMQTTGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVENGVIAYPVH
EITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=414, Percent_Identity=49.7584541062802, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47870; Mature: 47870

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVRLGEIEQIEFQQDKSLD
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCHHEEHCCCCCCCE
ITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCAGLADPELMAQHIGDPDLY
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC
HEWDLDTEAAVGFAKQCEQAALNEDERIENSEGAAVQTGHYQYVYGNTHGFAAHRQGTHH
CCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEECCCEEEEEECCCCCCCCCCCCCC
SISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQTAARRTLRRLGSRSIPTGSYPVL
EEEEEEEEECCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
FDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKKVLPDFLNLREEPHIPRSFRSSYFD
EECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
AEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQTTGNAGGAHNLYLNHTHETQSDLLKEM
CCCCCCCHHHHHHCCCCCEEEECCCCHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
GTGLLVTELMGQGANTITGDYSRGAAGFWVENGVIAYPVHEITVAGRLQDMYRDIVGVAD
CCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHH
DALRRSSNKIGSILIAGMTVAGS
HHHHHCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVRLGEIEQIEFQQDKSLD
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCHHEEHCCCCCCCE
ITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCAGLADPELMAQHIGDPDLY
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC
HEWDLDTEAAVGFAKQCEQAALNEDERIENSEGAAVQTGHYQYVYGNTHGFAAHRQGTHH
CCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEECCCEEEEEECCCCCCCCCCCCCC
SISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQTAARRTLRRLGSRSIPTGSYPVL
EEEEEEEEECCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
FDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKKVLPDFLNLREEPHIPRSFRSSYFD
EECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
AEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQTTGNAGGAHNLYLNHTHETQSDLLKEM
CCCCCCCHHHHHHCCCCCEEEECCCCHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
GTGLLVTELMGQGANTITGDYSRGAAGFWVENGVIAYPVHEITVAGRLQDMYRDIVGVAD
CCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHH
DALRRSSNKIGSILIAGMTVAGS
HHHHHCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]