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The map label for this gene is folK [H]

Identifier: 161869689

GI number: 161869689

Start: 717835

End: 718329

Strand: Reverse

Name: folK [H]

Synonym: NMCC_0709

Alternate gene names: 161869689

Gene position: 718329-717835 (Counterclockwise)

Preceding gene: 161869690

Following gene: 161869685

Centisome position: 33.36

GC content: 54.75

Gene sequence:

>495_bases
ATGAACAACAGACATTTTGCCGTCATCGCCCTGGGCAGTAATCTTGAAAACCCTGCCCAACAGGTACGCGCCGCATTGGA
CACGCTGTCGTCCCATCCTGACATCCGTCTTAAACAGGCTTCCTCACTGTATATGACCGCGCCCGTCGGTTACGACAATC
AGCCCGATTTCGTCAATGCCGTCTGCACCGTTTCCACCACCTTGGACGGCATTGCCCTGCTTGCCGAACTCAACCGTATC
GAAGCCGATTTCGGACGCGAACGCAGCTTCCGCAACGCGCCGCGCACATTGGATTTGGACATTATCGACTTTGACGGCAT
CTCCAGCGACGACCCCCGACTCACCCTGCCGCATCCGCGCGCGCACGAACGCAGTTTCGTCATACGCCCTTTGGCAGAAA
TCCTCCCTGATTTTATTTTGGGAAAACACGGAAAGGTTGCCGAATTGTCAAAACGGCTGGGCAATCAAGGCATCCGTCTT
TTACCGGATAAGTAA

Upstream 100 bases:

>100_bases
TTCGACCAAGTTCTACACCGTTTACCTGCTCGCCATGTGTTGCCTCGCCTGCATCGTTTACCTTGCCAAAACCAAAGTCC
TGCCTTTCTGAAACACCGTT

Downstream 100 bases:

>100_bases
TTCCGCACGCGGATGCCGTCTGAATGCCGGTTTCAGACGGCATTTTTTCTTTGCCGCCAACACGCGTGCAAAAAAATCGC
CCCTTGGAAAAGGGGCGCAA

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine- pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLYMTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRI
EADFGRERSFRNAPRTLDLDIIDFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRL
LPDK

Sequences:

>Translated_164_residues
MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLYMTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRI
EADFGRERSFRNAPRTLDLDIIDFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRL
LPDK
>Mature_164_residues
MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLYMTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRI
EADFGRERSFRNAPRTLDLDIIDFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRL
LPDK

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=135, Percent_Identity=42.962962962963, Blast_Score=101, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324073, Length=140, Percent_Identity=35, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 18164; Mature: 18164

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLYMTAPVGYDNQPDFVNA
CCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCCCCCCHHHH
VCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDIIDFDGISSDDPRLTLPHPR
EEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCEEECCCCC
AHERSFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRLLPDK
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEECCCC
>Mature Secondary Structure
MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLYMTAPVGYDNQPDFVNA
CCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCCCCCCHHHH
VCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDIIDFDGISSDDPRLTLPHPR
EEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCEEECCCCC
AHERSFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRLLPDK
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10080886 [H]