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The map label for this gene is metK [H]

Identifier: 161869420

GI number: 161869420

Start: 444761

End: 445930

Strand: Direct

Name: metK [H]

Synonym: NMCC_0428

Alternate gene names: 161869420

Gene position: 444761-445930 (Clockwise)

Preceding gene: 161869418

Following gene: 161869421

Centisome position: 20.65

GC content: 56.67

Gene sequence:

>1170_bases
ATGAGCGAATATCTGTTTACTTCCGAATCGGTATCCGAAGGCCATCCGGACAAAGTGGCCGACCAAGTATCCGATGCTAT
TTTGGATGCGATTCTGGCGCAAGACCCCAAAGCGCGTGTCGCCGCAGAAACTTTGGTCAACACAGGCTTGTGCGTATTGG
CGGGCGAAATCACCACCACCGCCCAAGTGGACTACATCAAAGTCGCACGCGAAACCATCAAACGCATCGGCTACAACTCC
TCCGAGCTGGGCTTTGATGCCAACGGCTGCGCGGTCGGCGTGTACTACGACCAACAATCTCCGGACATTGCCCAAGGCGT
AAACGAAGGCGAAGGCATCGACTTGAACCAAGGCGCAGGCGACCAAGGTCTGATGTTCGGCTACGCCTGCGACGAAACCC
CGACCCTGATGCCGTTTGCCATCTATTACAGCCACCGCCTGATGCAACGCCAAAGCGAATTGCGCAAAGACGGCCGCCTG
CCGTGGCTGCGTCCTGATGCCAAAGCCCAACTGACCGTGGTTTACGACAGCGAAACCGGTAAGGTAAAACGCATTGATAC
CGTCGTCCTCTCTACGCAACACGATCCGGAAATCGGTTATGAAGAGTTGAAAAACGCCGTCATTGAGCAGATTATCAAGC
CCGTCCTGCCGTCTGAACTGCTGACCGACGAAACCAAATACCTGATCAACCCGACCGGCCGCTTCGTCATCGGCGGCCCG
CAAGGCGACTGCGGTTTGACCGGCCGTAAAATCATCGTCGATACCTACGGCGGCGCGGCTCCGCACGGCGGCGGCGCGTT
CTCCGGCAAAGATCCGTCCAAGGTTGACCGTTCCGCCGCCTACGCCTGCCGCTATGTGGCGAAAAACATCGTCGCCGCAG
GCTTGGCAACCCAATGCCAAATCCAAGTTTCCTACGCCATCGGCGTTGCCGAACCGACTTCGATTTCCATCGATACTTTC
GGCACAGGCAAAATCAGCGAAGAAAAACTGATTACCTTAGTTCGCGAACATTTCGACCTGCGCCCCAAAGGCATCGTCCA
AATGCTCGACCTCTTGCGCCCGATTTACAGTAAATCCGCCGCTTACGGACATTTCGGCCGCGAAGAACCTGAGTTCACTT
GGGAGCGCACCGACAAAGCTGCTGCATTGAGGGCGGCAGCGGGGCTGTAA

Upstream 100 bases:

>100_bases
GGCACGAATAGGGAAATTTTGCTATATTTCCCGCTGTCGACATTATGTTCATACAACATGCCGTCTAACGAAGATGGTTT
GTTTTTCAAGGAAAATCTCA

Downstream 100 bases:

>100_bases
TTCCGGTTTGAAAATCAAAAATGCCGTCTGAACAGTTCAGACGGCATTTTTATATAGTGGATTAACAAAAATCAGGACAG
AGACCTTTGCAAAAATAGTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAETLVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNS
SELGFDANGCAVGVYYDQQSPDIAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPSELLTDETKYLINPTGRFVIGGP
QGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTF
GTGKISEEKLITLVREHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL

Sequences:

>Translated_389_residues
MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAETLVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNS
SELGFDANGCAVGVYYDQQSPDIAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPSELLTDETKYLINPTGRFVIGGP
QGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTF
GTGKISEEKLITLVREHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL
>Mature_388_residues
SEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAETLVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSS
ELGFDANGCAVGVYYDQQSPDIAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRLP
WLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPSELLTDETKYLINPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFG
TGKISEEKLITLVREHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=377, Percent_Identity=58.3554376657825, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI4557737, Length=377, Percent_Identity=58.6206896551724, Blast_Score=423, Evalue=1e-118,
Organism=Escherichia coli, GI1789311, Length=389, Percent_Identity=69.1516709511568, Blast_Score=548, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=57.1808510638298, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=56.1170212765958, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=55.7894736842105, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17551082, Length=380, Percent_Identity=53.4210526315789, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=50.531914893617, Blast_Score=353, Evalue=8e-98,
Organism=Caenorhabditis elegans, GI32565911, Length=329, Percent_Identity=55.0151975683891, Blast_Score=351, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI25145635, Length=332, Percent_Identity=53.6144578313253, Blast_Score=342, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI32565903, Length=302, Percent_Identity=53.6423841059603, Blast_Score=311, Evalue=5e-85,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=61.4457831325301, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=76.9230769230769, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=375, Percent_Identity=57.8666666666667, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6320710, Length=378, Percent_Identity=56.3492063492063, Blast_Score=423, Evalue=1e-119,
Organism=Drosophila melanogaster, GI45552159, Length=374, Percent_Identity=54.2780748663102, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580529, Length=374, Percent_Identity=54.2780748663102, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580537, Length=374, Percent_Identity=54.2780748663102, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580533, Length=374, Percent_Identity=54.2780748663102, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580535, Length=374, Percent_Identity=54.2780748663102, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580531, Length=374, Percent_Identity=54.2780748663102, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580527, Length=377, Percent_Identity=53.8461538461538, Blast_Score=373, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580525, Length=377, Percent_Identity=53.8461538461538, Blast_Score=373, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580541, Length=378, Percent_Identity=51.5873015873016, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=57.3770491803279, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42148; Mature: 42017

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAETLVNTGLCVLAGEITTT
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCCCC
AQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPDIAQGVNEGEGIDLNQGAG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCCCCCEECCCCCC
DQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRLPWLRPDAKAQLTVVYDSETG
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCC
KVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPSELLTDETKYLINPTGRFVIGGP
CEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCEEECCCCCEEEECC
QGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYACRYVAKNIVAAGLATQCQ
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IQVSYAIGVAEPTSISIDTFGTGKISEEKLITLVREHFDLRPKGIVQMLDLLRPIYSKSA
EEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
AYGHFGREEPEFTWERTDKAAALRAAAGL
CCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAETLVNTGLCVLAGEITTT
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCCCC
AQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPDIAQGVNEGEGIDLNQGAG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCCCCCEECCCCCC
DQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRLPWLRPDAKAQLTVVYDSETG
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCC
KVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPSELLTDETKYLINPTGRFVIGGP
CEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCEEECCCCCEEEECC
QGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYACRYVAKNIVAAGLATQCQ
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IQVSYAIGVAEPTSISIDTFGTGKISEEKLITLVREHFDLRPKGIVQMLDLLRPIYSKSA
EEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
AYGHFGREEPEFTWERTDKAAALRAAAGL
CCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA