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The map label for this gene is crgA [H]

Identifier: 161869360

GI number: 161869360

Start: 366222

End: 367037

Strand: Direct

Name: crgA [H]

Synonym: NMCC_0366

Alternate gene names: 161869360

Gene position: 366222-367037 (Clockwise)

Preceding gene: 161869358

Following gene: 161869368

Centisome position: 17.01

GC content: 51.59

Gene sequence:

>816_bases
ATGGCAAATTCTGCCGTAAGCCGCATCGTCAAACGGCTGGAGGAAAAGTTGGGTGTGAACCTGCTCAACCGCACCACGCG
GCAACTCAGTCTGACGGAAGAAGGCGCGCAATATTTCCGCCGCGCGCAGAGAATCCTGCAAGAAATGGCAGCGGCGGAAA
CCGAAATGCTGGCAGTGCACGAAATACCGCAAGGCGTGTTGCGCGTGGATTCCGCGATGCCGATGGTGCTGCATCTGCTG
GCGCCGCTGGCAACAAAATTCAACGAACGCTATCCGCATATCCGACTTTCGCTCGTTTCTTCCGAAGGCTATATCAATCT
GATTGAACGCAAAGTCGATATTGCCTTACGGGCCGGAGAATTGGACGATTCCGGGCTGCGTGCACGCCATCTGTTTGACA
GCCGCTTCCGCGTAATCGCCAGTCCTGAATACCTGGCAAAACACGGCACGCCGCAATCTACAGAAGAGCTTGCCGGTCAC
CAATGTTTAGGCTTCACCGAACCCGGTTCTCTAAATACATGGGCGGTTTTAGATGCGCAGGGAAATCCCTATAAGATTTC
ACCGCACTTTACCGCCAGCAGCGGTGAAATCTTACGCTCGTTGTGCCTTTCAGGTTGCGGTATTGCTTGCTTATCAGATT
TTTTGGTTGACAACGACATCGCTGAAGGAAAGTTAATTCCCCTGCTCGCCGAACAAACCTCCGATAAAACACACCCCTTT
AATGCTGTTTATTACAGCGATAAAGCCGTCAACCTCCGCTTGCGCGTATTTTTGGATTTTTTAGTGGAGGAACTGGGAAA
CAATCTCTGTGGATAA

Upstream 100 bases:

>100_bases
TCGAAAAGGAAACACGATGAAAACCAATTCAGAAGAACTGACCGTATTTGTTCAAGTGGTGGAAAGCGGCAGCTTCAGCC
GTGCGGCGGAGCAGTTGGCG

Downstream 100 bases:

>100_bases
TTCTATAAAAACCTGCCATAAAATTGTTTTTACTCACTTAATAACTTTGAGAAAAAATAAATAAGATGATGTATAGCCGG
CGGCAATGGCAGTCTATATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MANSAVSRIVKRLEEKLGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMPMVLHLL
APLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLFDSRFRVIASPEYLAKHGTPQSTEELAGH
QCLGFTEPGSLNTWAVLDAQGNPYKISPHFTASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPF
NAVYYSDKAVNLRLRVFLDFLVEELGNNLCG

Sequences:

>Translated_271_residues
MANSAVSRIVKRLEEKLGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMPMVLHLL
APLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLFDSRFRVIASPEYLAKHGTPQSTEELAGH
QCLGFTEPGSLNTWAVLDAQGNPYKISPHFTASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPF
NAVYYSDKAVNLRLRVFLDFLVEELGNNLCG
>Mature_270_residues
ANSAVSRIVKRLEEKLGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMPMVLHLLA
PLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLFDSRFRVIASPEYLAKHGTPQSTEELAGHQ
CLGFTEPGSLNTWAVLDAQGNPYKISPHFTASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFN
AVYYSDKAVNLRLRVFLDFLVEELGNNLCG

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=264, Percent_Identity=58.7121212121212, Blast_Score=331, Evalue=3e-92,
Organism=Escherichia coli, GI87081978, Length=260, Percent_Identity=33.0769230769231, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789639, Length=270, Percent_Identity=30.7407407407407, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789440, Length=260, Percent_Identity=29.2307692307692, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI145693193, Length=266, Percent_Identity=28.1954887218045, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787128, Length=264, Percent_Identity=25.7575757575758, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787589, Length=249, Percent_Identity=27.3092369477912, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1789173, Length=256, Percent_Identity=26.953125, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788706, Length=244, Percent_Identity=24.5901639344262, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1786448, Length=267, Percent_Identity=25.4681647940075, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=98, Percent_Identity=33.6734693877551, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30064; Mature: 29933

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSAVSRIVKRLEEKLGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVH
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIPQGVLRVDSAMPMVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGE
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHEEECCC
LDDSGLRARHLFDSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQ
CCCCCCHHHHHHHHHHHEEECHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECC
GNPYKISPHFTASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPF
CCCEEECCCEECCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCCCC
NAVYYSDKAVNLRLRVFLDFLVEELGNNLCG
EEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ANSAVSRIVKRLEEKLGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVH
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIPQGVLRVDSAMPMVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGE
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHEEECCC
LDDSGLRARHLFDSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQ
CCCCCCHHHHHHHHHHHEEECHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECC
GNPYKISPHFTASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPF
CCCEEECCCEECCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCCCC
NAVYYSDKAVNLRLRVFLDFLVEELGNNLCG
EEEEECCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]