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The map label for this gene is queA [H]

Identifier: 161869357

GI number: 161869357

Start: 363807

End: 364847

Strand: Direct

Name: queA [H]

Synonym: NMCC_0363

Alternate gene names: 161869357

Gene position: 363807-364847 (Clockwise)

Preceding gene: 161869356

Following gene: 161869358

Centisome position: 16.89

GC content: 57.83

Gene sequence:

>1041_bases
ATGGATATTTCAGATTTTGATTTTACCCTGCCCGAAAAGCTGATTGCCCAGCATCCGCCCGAAGTGCGCGGCAGCAGCCG
CCTTTTGGTTGCGTTGCCCGATATGCCGCTGCAAGACCGGGTGTTCGGCGATTTGCCGGATTATGTCGAGGCGGGCGACG
TTTTGGTGTTCAACAACACCAAAGTCATGAAGGCGCGGCTGTTTGGGCAGAAAGACAGCGGCGGCAGGATCGAAGCCCTG
ATCGAGCGTGTTTTAGACAGTCATACCGCACTGGCGCACATCCGTTCGTCCAAGTCCCCCAAGCCCGGTATGGGGCTGGT
GTTTGAAGGCGGCATCCGCGCCGTGATGGTCGGGCGTGAGGGCGAACTGTTCTGCCTGCGTTTTGAAGGCGGAGAGACCG
TTTACGAACTTTTGGAACAGAACGGACACCTGCCCCTGCCGCCTTATATCGAACGTGCCGCCGATGCGGACGACGACAGC
CGTTATCAAACCGTTTATGCCAAATATCAGGGCGCGGTCGCCGCGCCGACGGCGGGCCTGCATTTTACGGAAGAACTTTT
GCGCCGTCTGAAAGACAAAGGCGCGGTAACCGCAGAAGTAACCCTGCACGTCGGTGCGGGAACGTTCCAACCCGTGCGCG
TCGATAAAATCGAAGAACACAAAATGCACAGCGAATGGTTTGAAGTGCCGTCTGAAACCGCCGCCGCCGTTGAGGCGGCA
AAAGCCCGGGGGAACAAAGTCTGGGCGGTCGGCACGACTTCCATGCGCGCCCTCGAATCTGCCGCGCGTGCGACGGGTCG
TCTGAAAGCGGGGCAGGGCGACACCGATATTTTCATCACGCCGGGCTACCGTTTTAATGTTGTCGACAGACTGGTTACCA
ATTTTCATTTGCCGAAATCGACGCTGCTGATGTTGGTCAGCGCGTTTTCGGGTATGGGTCATATCCGCGCCGCGTATCGT
CATGCGGTCGAACGCGAATACCGTTTCTTCAGCTACGGCGATGCGATGGTTTTGGGGCGGAACGAAGGGGTGGTACGTTA
G

Upstream 100 bases:

>100_bases
AGAGTGTTTATTCTAATCTGTTGGTTTTTCGGGCAAAGATGCCGTCTGAAAAAGGGCTAAAGTGCGTATAATGGCGGCTT
GCCCGAACGAGAGTGTAAAA

Downstream 100 bases:

>100_bases
AACAGCCTCAAGTCTATTGTGGGAAGACAAGTGCCGTCTGAACGGACGTTCGGACGGCATTCTTTTGTTGATTTGAATGA
AAGGAACTGCATGACATATA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGDLPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEAL
IERVLDSHTALAHIRSSKSPKPGMGLVFEGGIRAVMVGREGELFCLRFEGGETVYELLEQNGHLPLPPYIERAADADDDS
RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIEEHKMHSEWFEVPSETAAAVEAA
KARGNKVWAVGTTSMRALESAARATGRLKAGQGDTDIFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYR
HAVEREYRFFSYGDAMVLGRNEGVVR

Sequences:

>Translated_346_residues
MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGDLPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEAL
IERVLDSHTALAHIRSSKSPKPGMGLVFEGGIRAVMVGREGELFCLRFEGGETVYELLEQNGHLPLPPYIERAADADDDS
RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIEEHKMHSEWFEVPSETAAAVEAA
KARGNKVWAVGTTSMRALESAARATGRLKAGQGDTDIFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYR
HAVEREYRFFSYGDAMVLGRNEGVVR
>Mature_346_residues
MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGDLPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEAL
IERVLDSHTALAHIRSSKSPKPGMGLVFEGGIRAVMVGREGELFCLRFEGGETVYELLEQNGHLPLPPYIERAADADDDS
RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIEEHKMHSEWFEVPSETAAAVEAA
KARGNKVWAVGTTSMRALESAARATGRLKAGQGDTDIFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYR
HAVEREYRFFSYGDAMVLGRNEGVVR

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=56.9364161849711, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38191; Mature: 38191

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGDLPDYVEAGDVLVFNNT
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCHHHCCCCCCHHCCCCEEEECCC
KVMKARLFGQKDSGGRIEALIERVLDSHTALAHIRSSKSPKPGMGLVFEGGIRAVMVGRE
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEEECC
GELFCLRFEGGETVYELLEQNGHLPLPPYIERAADADDDSRYQTVYAKYQGAVAAPTAGL
CCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHEEEEEHHCCCEECCCCCH
HFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIEEHKMHSEWFEVPSETAAAVEAA
HHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEECCHHHHHHHHHHHHCCCCHHHHHHHHH
KARGNKVWAVGTTSMRALESAARATGRLKAGQGDTDIFITPGYRFNVVDRLVTNFHLPKS
HHCCCEEEEEECHHHHHHHHHHHHHCCEECCCCCCEEEECCCCEEHHHHHHHHHCCCCHH
TLLMLVSAFSGMGHIRAAYRHAVEREYRFFSYGDAMVLGRNEGVVR
HHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCC
>Mature Secondary Structure
MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGDLPDYVEAGDVLVFNNT
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCHHHCCCCCCHHCCCCEEEECCC
KVMKARLFGQKDSGGRIEALIERVLDSHTALAHIRSSKSPKPGMGLVFEGGIRAVMVGRE
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEEECC
GELFCLRFEGGETVYELLEQNGHLPLPPYIERAADADDDSRYQTVYAKYQGAVAAPTAGL
CCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHEEEEEHHCCCEECCCCCH
HFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIEEHKMHSEWFEVPSETAAAVEAA
HHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEECCHHHHHHHHHHHHCCCCHHHHHHHHH
KARGNKVWAVGTTSMRALESAARATGRLKAGQGDTDIFITPGYRFNVVDRLVTNFHLPKS
HHCCCEEEEEECHHHHHHHHHHHHHCCEECCCCCCEEEECCCCEEHHHHHHHHHCCCCHH
TLLMLVSAFSGMGHIRAAYRHAVEREYRFFSYGDAMVLGRNEGVVR
HHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA