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The map label for this gene is hemL

Identifier: 161869351

GI number: 161869351

Start: 358147

End: 359430

Strand: Direct

Name: hemL

Synonym: NMCC_0357

Alternate gene names: 161869351

Gene position: 358147-359430 (Clockwise)

Preceding gene: 161869350

Following gene: 161869356

Centisome position: 16.63

GC content: 58.8

Gene sequence:

>1284_bases
ATGAACCGTAACGAAATTTTATTCGACCGCGCCAAAGCCATCATCCCCGGCGGCGTGAATTCCCCCGTCCGCGCATTCGG
CAGCGTCGGCGGCGTGCCGCGCTTCATCAAAAAAGCCGAAGGCGCGTATGTTTGGGACGAAAACGGCACGCGTTATACCG
ACTATGTCGGCTCTTGGGGGCCTGCGATTGTCGGACACGCGCACCCCGAAGTCGTCGAAGCCGTGCGCGAAGCCGCGTTG
GGCGGTTTGTCGTTCGGCGCGCCCACCGAGGGCGAAATCGCCATTGCCGAACAAATCGCCAAAATCATGCCGTCCGTCGA
ACGGCTGCGCCTCGTCAGCTCCGGTACCGAAGCGACGATGACCGCCATCCGTTTGGCACGCGGTTTTACCGGTCGCGACA
AAATCATCAAGTTTGAAGGCTGCTACCACGGGCATTCCGACAGCCTGCTGGTGAAAGCCGGCAGCGGCCTGCTGACGTTC
GGCAATCCTTCTTCCGCCGGTGTGCCTGCCGACTTTACCAAACACACTTTAGTCCTTGAGTACAACAACATCGCCCAATT
GGAAGAAGCCTTTGCTCAAAGCGGCAATGACATTGCCTGTGTGATTTTGGAGCCTTTTGTCGGCAATATGAACCTTGTCC
GCCCGACCGAAGCCTTCGTCAAAGCCCTGCGCGAATTGACCGAAAAACACGGCGCGGTGTTGATTTACGACGAAGTGATG
ACCGGTTTCCGCGTCGCGCTCGGCGGCGCACAATCGCTGCACGGCATCATGCCCGACCTGACCACGATGGGCAAAGTCAT
CGGCGGCGGTATGCCGCTTGCCGCGTTCGGCGGGCGCAAAGACATTATGGAATGTATTTCCCCACTGGGCGGCGTGTATC
AGGCAGGCACCTTGTCGGGCAACCCGATTGCCGTCGCCGCCGGTTTGAAAACGCTGGAAATCATCCGCCGCGAAGGGTTT
TATGAAAACCTGACGGCGCGTACCGAACAGTTGGTTCAAGGTTTTCGGACGGCAGCGGATGCGGCGGGCATCGAGTTCAC
CGCCGACAGCGTGGGCGGTATGTTCGGTCTGTATTTCGCCGCACACGCGCCGCGAAACTATGCCGATATGGCGCGTTCCA
ATATTGACGCTTTCAAACGCTTCTTCCACGGTATGCTCGACCGCGGCATTGCCTTCGGCCCGTCCGCTTATGAAGCAGGT
TTCGTTTCTGCCGCGCATACGCCCGAGCTGATTGACGAAACGGTTGCGGTTGCGGTTGAAGTGTTCAAGGCGATGGCTGC
ATGA

Upstream 100 bases:

>100_bases
AGAAGCAGGCAAATCCTTGTCGCAACCTGAAAAATGATAAAATGGGACTTTCTGCTTCTAAGCCCGAAACCTGCCGTTCA
GACGGCATTTGAGGATAAAT

Downstream 100 bases:

>100_bases
TGTTTTGACGGACAGAGTTTCTCTGTTCGATTTGTTTGGCAGATTGAAGTAAGAATGCACACCGCCGTCATTCCCGCGCA
GGCGGGAATCCAGACCTTTC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYVWDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAAL
GGLSFGAPTEGEIAIAEQIAKIMPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEAFVKALRELTEKHGAVLIYDEVM
TGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAFGGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGF
YENLTARTEQLVQGFRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAFGPSAYEAG
FVSAAHTPELIDETVAVAVEVFKAMAA

Sequences:

>Translated_427_residues
MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYVWDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAAL
GGLSFGAPTEGEIAIAEQIAKIMPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEAFVKALRELTEKHGAVLIYDEVM
TGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAFGGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGF
YENLTARTEQLVQGFRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAFGPSAYEAG
FVSAAHTPELIDETVAVAVEVFKAMAA
>Mature_427_residues
MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYVWDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAAL
GGLSFGAPTEGEIAIAEQIAKIMPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEAFVKALRELTEKHGAVLIYDEVM
TGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAFGGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGF
YENLTARTEQLVQGFRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAFGPSAYEAG
FVSAAHTPELIDETVAVAVEVFKAMAA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=300, Percent_Identity=27.3333333333333, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI13994255, Length=328, Percent_Identity=29.2682926829268, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI284507298, Length=216, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=56.5727699530516, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1789016, Length=358, Percent_Identity=33.2402234636872, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788044, Length=346, Percent_Identity=30.0578034682081, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787560, Length=317, Percent_Identity=32.807570977918, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789759, Length=310, Percent_Identity=28.0645161290323, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI145693181, Length=313, Percent_Identity=29.3929712460064, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786991, Length=421, Percent_Identity=26.3657957244656, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71992977, Length=331, Percent_Identity=28.7009063444109, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI25144271, Length=320, Percent_Identity=25.9375, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32564660, Length=327, Percent_Identity=27.82874617737, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=159, Percent_Identity=28.3018867924528, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323470, Length=328, Percent_Identity=29.2682926829268, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=325, Percent_Identity=23.3846153846154, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21357415, Length=306, Percent_Identity=28.4313725490196, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356575, Length=359, Percent_Identity=26.7409470752089, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_NEIM0 (A9M1G6)

Other databases:

- EMBL:   CP000381
- RefSeq:   YP_001598518.1
- ProteinModelPortal:   A9M1G6
- SMR:   A9M1G6
- EnsemblBacteria:   EBNEIT00000012074
- GeneID:   5795341
- GenomeReviews:   CP000381_GR
- KEGG:   nmn:NMCC_0357
- GeneTree:   EBGT00050000020132
- HOGENOM:   HBG725944
- OMA:   DKVIKFE
- ProtClustDB:   PRK00062
- BioCyc:   NMEN374833:NMCC_0357-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45340; Mature: 45340

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYVWDENGTRYTDYVGSWG
CCCCCCHHHHHHEECCCCCCHHHHHHHCCCCHHHHHHHCCCCEEECCCCCEECCCCCCCC
PAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKIMPSVERLRLVSSGTEATM
CCHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHH
TAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTFGNPSSAGVPADFTKHTLVLE
HHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCEEEEE
YNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEAFVKALRELTEKHGAVLIYDEVM
ECCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEHHHH
TGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAFGGRKDIMECISPLGGVYQAGTLSG
HHHHHHHCCHHHHHHCCCCHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHCCCEECCCCCC
NPIAVAAGLKTLEIIRREGFYENLTARTEQLVQGFRTAADAAGIEFTADSVGGMFGLYFA
CCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHH
AHAPRNYADMARSNIDAFKRFFHGMLDRGIAFGPSAYEAGFVSAAHTPELIDETVAVAVE
HCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHH
VFKAMAA
HHHHHCC
>Mature Secondary Structure
MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYVWDENGTRYTDYVGSWG
CCCCCCHHHHHHEECCCCCCHHHHHHHCCCCHHHHHHHCCCCEEECCCCCEECCCCCCCC
PAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAKIMPSVERLRLVSSGTEATM
CCHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHH
TAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTFGNPSSAGVPADFTKHTLVLE
HHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCEEEEE
YNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEAFVKALRELTEKHGAVLIYDEVM
ECCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEHHHH
TGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAFGGRKDIMECISPLGGVYQAGTLSG
HHHHHHHCCHHHHHHCCCCHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHCCCEECCCCCC
NPIAVAAGLKTLEIIRREGFYENLTARTEQLVQGFRTAADAAGIEFTADSVGGMFGLYFA
CCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHH
AHAPRNYADMARSNIDAFKRFFHGMLDRGIAFGPSAYEAGFVSAAHTPELIDETVAVAVE
HCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHH
VFKAMAA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA