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The map label for this gene is nusA [H]

Identifier: 161751102

GI number: 161751102

Start: 287320

End: 287751

Strand: Reverse

Name: nusA [H]

Synonym: ST0273

Alternate gene names: 161751102

Gene position: 287751-287320 (Counterclockwise)

Preceding gene: 15920463

Following gene: 15920461

Centisome position: 10.68

GC content: 32.18

Gene sequence:

>432_bases
TTGCCTGAGATTAAGCTTACTTCAGAGGAATTAAGGTATATGTCACTTTTTCAAGACGTAACGCGTGTAACTGCAAGGGA
TTGTATTGTAGATGAAGAAAATAACAGAATTATATTTCTTGTAAACCCAGAAAATATGGGAACAGCTATAGGTAAAAATG
GTTCTAATGTAAAAAGGCTGGAAAAACTTATAGGAAAATCAATTGAGATAGTAGGATATAGCGATAATCTAGAAGATTTA
GTAAGAAATCTAATGGCACCTGCAAAGGTGAGAAGTGTAAAAGTGGTTCAGTCTAATTCTAAGAAGACAGTTTATATTAA
TGTTGATCCTCAAGATAAGGGCCTTGCTATTGGTAAGAACGGAAGAAATGTTACTAGAGCAAAGTTAATATTAAAAAGAT
ATATGGATATTGATTCCGTGATTATAACTTAA

Upstream 100 bases:

>100_bases
AGGAACACTTTGTGGAAAACCATTTATGATATCCACTATTGGTGTTATAGATCCTGGTGAATCCAGATTACTAGAAGAAA
TAAAGGAGGGGGCTCAATAA

Downstream 100 bases:

>100_bases
GGGTGAAGAAGAATGGCAGGAGGTAAATCACCTAAAGGAATGTTTGCTGCAAGGAAATTAAGGTTAAAGAGACTTAAGTT
TAGATGGCATCAAAGAAAAT

Product: transcription elongation factor NusA-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MPEIKLTSEELRYMSLFQDVTRVTARDCIVDEENNRIIFLVNPENMGTAIGKNGSNVKRLEKLIGKSIEIVGYSDNLEDL
VRNLMAPAKVRSVKVVQSNSKKTVYINVDPQDKGLAIGKNGRNVTRAKLILKRYMDIDSVIIT

Sequences:

>Translated_143_residues
MPEIKLTSEELRYMSLFQDVTRVTARDCIVDEENNRIIFLVNPENMGTAIGKNGSNVKRLEKLIGKSIEIVGYSDNLEDL
VRNLMAPAKVRSVKVVQSNSKKTVYINVDPQDKGLAIGKNGRNVTRAKLILKRYMDIDSVIIT
>Mature_142_residues
PEIKLTSEELRYMSLFQDVTRVTARDCIVDEENNRIIFLVNPENMGTAIGKNGSNVKRLEKLIGKSIEIVGYSDNLEDLV
RNLMAPAKVRSVKVVQSNSKKTVYINVDPQDKGLAIGKNGRNVTRAKLILKRYMDIDSVIIT

Specific function: Participates In Both The Termination And Antitermination Of Transcription. Nusa Binds Directly To The Core Enzyme Of The DNA-Dependent RNA Polymerase. Nusa Also Interacts With Lambda N Protein, RNA, Rho Factor, And Perhaps Nusb. Binds RNA. [C]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KH domain [H]

Homologues:

None

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR004044
- InterPro:   IPR010212 [H]

Pfam domain/function: PF07650 KH_2 [H]

EC number: NA

Molecular weight: Translated: 16096; Mature: 15965

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEIKLTSEELRYMSLFQDVTRVTARDCIVDEENNRIIFLVNPENMGTAIGKNGSNVKRL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCCCCCHHHH
EKLIGKSIEIVGYSDNLEDLVRNLMAPAKVRSVKVVQSNSKKTVYINVDPQDKGLAIGKN
HHHHCCEEEEEECCCCHHHHHHHHCCCHHHHEEEEEECCCCEEEEEEECCCCCCEEECCC
GRNVTRAKLILKRYMDIDSVIIT
CCCHHHHHHHHHHHCCCCCEEEC
>Mature Secondary Structure 
PEIKLTSEELRYMSLFQDVTRVTARDCIVDEENNRIIFLVNPENMGTAIGKNGSNVKRL
CCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCCCCCHHHH
EKLIGKSIEIVGYSDNLEDLVRNLMAPAKVRSVKVVQSNSKKTVYINVDPQDKGLAIGKN
HHHHCCEEEEEECCCCHHHHHHHHCCCHHHHEEEEEECCCCEEEEEEECCCCCCEEECCC
GRNVTRAKLILKRYMDIDSVIIT
CCCHHHHHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2501756 [H]