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The map label for this gene is rumA

Identifier: 161702959

GI number: 161702959

Start: 2161136

End: 2162533

Strand: Reverse

Name: rumA

Synonym: Bcep18194_A5118

Alternate gene names: 161702959

Gene position: 2162533-2161136 (Counterclockwise)

Preceding gene: 78066589

Following gene: 78066585

Centisome position: 58.54

GC content: 66.81

Gene sequence:

>1398_bases
GTGTCCGAAGCCGTCCCCACTTCTGCGCGCAAATCAAGAAATGCGCCCGTCGCGCCCGGGCCCGCCCCGGTTCTCGAGAT
CGAATCGCTCGACATGGAAGCGCGCGGTGTCGGTCGCACGATCACCGAGGACGGCGAGCCTGGCAAGGTCATCTTCGTCG
AAGGCGCGCTGCCGGGCGAGCGCGTGACCTATTCGAGCTTCCGCCGCAAGCCAAGCTACGAGCAGGCGACGGTTGTCGAC
ATTCTGCGCCCGAGCGTGATGCGCACGAAGCCGAAATGCGCGTTCTTCGGCACCTGCGGCGGTTGCTCGATGCAGCACCT
CGACATGCGTGCACAGGTGGCGGTCAAGCAGCGCGTGCTCGAAGACAACCTTTGGCACCTGGCGAAGCTGCGTGCGGAAA
CGATGTTCGCGCCGATCCACGGCCCGTCGTGGGGCTATCGCTACCGCGCGCGCCTGACCGTGCGCAACGTCGCGAAGAAG
GGCGGCGTGCTGGTCGGGTTCCACGAGAAGAAGAGCAGCTACGTCGCCGACATGACGAGCTGCGAAGTGCTGCCGCCGCA
CGTGTCGGCGATGCTCGTGCCGCTGCGCCGGCTCGTCGAGGGGCTGTCGATCCGCGATCGGATGCCGCAGATCGAACTCG
CGGTCGGTTCGGAAGTTACGGCGCTCGTGCTGCGCGTGCTGGAGCCGATCAACGCGGACGACGAAGCGCTGCTGCGCGCG
TTCGCTGACGAGCATAAAGTGCAGTTCTGGCTGCAGCCGAAAGGTCCGGACACGGTGGCGCCGTTCTATCCGCTGGACGT
GTCGCTCGACTACACGCTGCCGGAATTCGGCATCCGCATGCCGTTCAAGCCGACCGACTTCACGCAGGTCAACCACCAGA
TCAACCGCGTGCTGGTAGGCCGCGCGCTGCGTCTGCTCGCGCCGTCGCGCGACGACCGCGTGCTCGACCTGTTCTGCGGG
ATCGGCAACTTCACGCTGCCGCTCGCGCGGCTGTCGCGCGAGGTGATGGGCATCGAAGGCAGTGACACGCTGACGACGCG
CGCGCTGGCGAACGCGCGCGAGAACGGTGTCGACGGCCACACGACGTTCGCGTGCCGTAACCTGTTCGAAGTGACGGGCG
ACGACATCCGCGCGCTCGGCGCATTCGACAAGTTCCTGATCGACCCACCGCGCGAAGGCGCGCTCGCGGTGTCGAAGGCG
CTGGCCGAGATCGCGCAGAGCGGTGAAGGCCCGCTGCCGAAGCGCATCGTCTACGTGTCGTGCAACCCGTCGACGCTCGC
GCGCGATGCGGGCCTGCTCGTGCACGAGGCCGGCTACCGCCTGAAGGGCGCCGGTGTCGTGAACATGTTCCCGAATACGT
CGCACGTCGAATCGATCGCACTGTTCGAGCGCGACTGA

Upstream 100 bases:

>100_bases
GTCTATGCTCAAGTATCGACGCTTGCCGCATGCAAAGCGGGCGATGCGGGCCTGCGTCGTCTACAATCTCGCCTTCTTCA
ACGTTTGTCAGGAAAAGCTG

Downstream 100 bases:

>100_bases
GCCCAAGCGGCGGACGTAAAAAAACCGGCCCGCGGGCCGGTTTTTTCATGGGGTGAGACGAACGTCAGTTGCGGTTGCCG
CCGAAGATGCCGAGCAGCGC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSEAVPTSARKSRNAPVAPGPAPVLEIESLDMEARGVGRTITEDGEPGKVIFVEGALPGERVTYSSFRRKPSYEQATVVD
ILRPSVMRTKPKCAFFGTCGGCSMQHLDMRAQVAVKQRVLEDNLWHLAKLRAETMFAPIHGPSWGYRYRARLTVRNVAKK
GGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSEVTALVLRVLEPINADDEALLRA
FADEHKVQFWLQPKGPDTVAPFYPLDVSLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVGRALRLLAPSRDDRVLDLFCG
IGNFTLPLARLSREVMGIEGSDTLTTRALANARENGVDGHTTFACRNLFEVTGDDIRALGAFDKFLIDPPREGALAVSKA
LAEIAQSGEGPLPKRIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVESIALFERD

Sequences:

>Translated_465_residues
MSEAVPTSARKSRNAPVAPGPAPVLEIESLDMEARGVGRTITEDGEPGKVIFVEGALPGERVTYSSFRRKPSYEQATVVD
ILRPSVMRTKPKCAFFGTCGGCSMQHLDMRAQVAVKQRVLEDNLWHLAKLRAETMFAPIHGPSWGYRYRARLTVRNVAKK
GGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSEVTALVLRVLEPINADDEALLRA
FADEHKVQFWLQPKGPDTVAPFYPLDVSLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVGRALRLLAPSRDDRVLDLFCG
IGNFTLPLARLSREVMGIEGSDTLTTRALANARENGVDGHTTFACRNLFEVTGDDIRALGAFDKFLIDPPREGALAVSKA
LAEIAQSGEGPLPKRIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVESIALFERD
>Mature_464_residues
SEAVPTSARKSRNAPVAPGPAPVLEIESLDMEARGVGRTITEDGEPGKVIFVEGALPGERVTYSSFRRKPSYEQATVVDI
LRPSVMRTKPKCAFFGTCGGCSMQHLDMRAQVAVKQRVLEDNLWHLAKLRAETMFAPIHGPSWGYRYRARLTVRNVAKKG
GVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSEVTALVLRVLEPINADDEALLRAF
ADEHKVQFWLQPKGPDTVAPFYPLDVSLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVGRALRLLAPSRDDRVLDLFCGI
GNFTLPLARLSREVMGIEGSDTLTTRALANARENGVDGHTTFACRNLFEVTGDDIRALGAFDKFLIDPPREGALAVSKAL
AEIAQSGEGPLPKRIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVESIALFERD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=197, Percent_Identity=29.4416243654822, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI51173878, Length=197, Percent_Identity=29.4416243654822, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789148, Length=445, Percent_Identity=36.1797752808989, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1787083, Length=402, Percent_Identity=24.3781094527363, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790403, Length=203, Percent_Identity=27.0935960591133, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24666579, Length=228, Percent_Identity=30.7017543859649, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 51112; Mature: 50980

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAVPTSARKSRNAPVAPGPAPVLEIESLDMEARGVGRTITEDGEPGKVIFVEGALPGE
CCCCCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
RVTYSSFRRKPSYEQATVVDILRPSVMRTKPKCAFFGTCGGCSMQHLDMRAQVAVKQRVL
CCCHHHHHCCCCCCHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
EDNLWHLAKLRAETMFAPIHGPSWGYRYRARLTVRNVAKKGGVLVGFHEKKSSYVADMTS
HHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEEHHHHHHHCCEEEEEECCCCCHHHHCCC
CEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSEVTALVLRVLEPINADDEALLRA
CCCCCHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHH
FADEHKVQFWLQPKGPDTVAPFYPLDVSLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVG
HHCCCEEEEEECCCCCCCCCCEEEEEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHH
RALRLLAPSRDDRVLDLFCGIGNFTLPLARLSREVMGIEGSDTLTTRALANARENGVDGH
HHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
TTFACRNLFEVTGDDIRALGAFDKFLIDPPREGALAVSKALAEIAQSGEGPLPKRIVYVS
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEE
CNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVESIALFERD
CCCHHHHHHCCEEEECCCCEEECCCEEEECCCCCCHHEEEEEECC
>Mature Secondary Structure 
SEAVPTSARKSRNAPVAPGPAPVLEIESLDMEARGVGRTITEDGEPGKVIFVEGALPGE
CCCCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
RVTYSSFRRKPSYEQATVVDILRPSVMRTKPKCAFFGTCGGCSMQHLDMRAQVAVKQRVL
CCCHHHHHCCCCCCHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
EDNLWHLAKLRAETMFAPIHGPSWGYRYRARLTVRNVAKKGGVLVGFHEKKSSYVADMTS
HHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEEHHHHHHHCCEEEEEECCCCCHHHHCCC
CEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSEVTALVLRVLEPINADDEALLRA
CCCCCHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHH
FADEHKVQFWLQPKGPDTVAPFYPLDVSLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVG
HHCCCEEEEEECCCCCCCCCCEEEEEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHH
RALRLLAPSRDDRVLDLFCGIGNFTLPLARLSREVMGIEGSDTLTTRALANARENGVDGH
HHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
TTFACRNLFEVTGDDIRALGAFDKFLIDPPREGALAVSKALAEIAQSGEGPLPKRIVYVS
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEE
CNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVESIALFERD
CCCHHHHHHCCEEEECCCCEEECCCEEEECCCCCCHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA