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The map label for this gene is nadD [C]

Identifier: 161611219

GI number: 161611219

Start: 598483

End: 599568

Strand: Reverse

Name: nadD [C]

Synonym: MHJ_0473

Alternate gene names: 161611219

Gene position: 599568-598483 (Counterclockwise)

Preceding gene: 71893825

Following gene: 71893823

Centisome position: 66.81

GC content: 29.47

Gene sequence:

>1086_bases
ATGACTTTGAAAAAGATTGCTATTTATGGAGGGACTTTTAACCCTGTTCACAAAGCGCATTTAGAAATTGCTCAAAAAGC
AATAGAATTTTTAAATCTTGATAAACTCTTTTTTGTCCCCAATTACATAAATCCGCTTAAGAAGCAAAAAAAAAATATTA
TTGATCCAAAATTCCGCTATGAGATGCTCAAATTGGTTCAAATTGAAAAAACAGATGTTTGTGATTTTGAAATTAAAGCG
AAAAAAGTTAGTTATACAATCGATACCATTAATTTTTTTGAAAAAAAATACCCTAATACAAAGATCTTTTTAATTATTGG
TTCCGATAATCTTCGTAATTTTAAATTATGGAAAAATTATAAAGAAATTCTTGAAAAAGTTCAACTTGTTGTTTTTACCC
GTAAAAATTATCCAGATTTACGCCAACTCAAACGTTATAACGGTTTAATTTTACCAACGAAATTGCCAAATTTCAGCTCA
TCACAAATTAGAAAAGGTGAATTTTCCGGTCTTGATTCAAAAGTTAGAGCTTATATAGGTAAAAACTTTCTTTATGCCGA
AGAAATTTTAACAAGTTTTTTAGGTTATTCCAAGCGCTTAATTCATTCCAAAAATACGGCCAAACTAGCAAATGAGTATG
CAAAAATTAACAATTTGGACTCTAAATTAGCTTATTATGCTGGATTATTTCACGATCTTACAAAGAAATGAACAAAAGAT
CAACATCTCGAATTTCTAAGAAGTCAAAAAATTTCAGTTAAGAATTTACAAGAATATCAACTTCATCAACTTTCGGCTTC
AATTTGACTTAAAAATGTTTATTGTTTACCTTTTGAGCAAATTATTTATGCAATTTCATGTCATACGAGCCTCTGTTTTG
AGATGTCCTTATTTGATAAGATTGTTTATGTCGCCGATAAATTAGCCCGCGGTCGGCGGTTTCTAGGAGTGCAAAAATTA
CGAAAATTAGCAAGACAGGATCTAGAAAAAGCATTTCAAAAATTAGTTGAAATCGCCTATAACAACCATAAAGATTTACC
AAATTCACGAGAGCAAAACAAAATTTATGCTAGACACCAAAATTAA

Upstream 100 bases:

>100_bases
CCGCCGATTTTTAAAAAATCCAGAAAAAGAAATTGCCAAATCAAAAAAAATTAAGGAAATACGTTCCACTGTCACAGTCG
GAGCAATTGATTATTAAAAG

Downstream 100 bases:

>100_bases
ATCAGCAATTTTTTATGCCGATACTAATGAAGCAGCAAACTTAGAAGAATTCGGCGCAATTTTGTTAAGAAATATTGAAA
TTTTTCAAAAAAAAATTGCT

Product: putative nicotinate-nucleotide adenylyltransferase

Products: diphosphate; deamido-NAD+

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTLKKIAIYGGTFNPVHKAHLEIAQKAIEFLNLDKLFFVPNYINPLKKQKKNIIDPKFRYEMLKLVQIEKTDVCDFEIKA
KKVSYTIDTINFFEKKYPNTKIFLIIGSDNLRNFKLWKNYKEILEKVQLVVFTRKNYPDLRQLKRYNGLILPTKLPNFSS
SQIRKGEFSGLDSKVRAYIGKNFLYAEEILTSFLGYSKRLIHSKNTAKLANEYAKINNLDSKLAYYAGLFHDLTKKWTKD
QHLEFLRSQKISVKNLQEYQLHQLSASIWLKNVYCLPFEQIIYAISCHTSLCFEMSLFDKIVYVADKLARGRRFLGVQKL
RKLARQDLEKAFQKLVEIAYNNHKDLPNSREQNKIYARHQN

Sequences:

>Translated_361_residues
MTLKKIAIYGGTFNPVHKAHLEIAQKAIEFLNLDKLFFVPNYINPLKKQKKNIIDPKFRYEMLKLVQIEKTDVCDFEIKA
KKVSYTIDTINFFEKKYPNTKIFLIIGSDNLRNFKLWKNYKEILEKVQLVVFTRKNYPDLRQLKRYNGLILPTKLPNFSS
SQIRKGEFSGLDSKVRAYIGKNFLYAEEILTSFLGYSKRLIHSKNTAKLANEYAKINNLDSKLAYYAGLFHDLTKK*TKD
QHLEFLRSQKISVKNLQEYQLHQLSASI*LKNVYCLPFEQIIYAISCHTSLCFEMSLFDKIVYVADKLARGRRFLGVQKL
RKLARQDLEKAFQKLVEIAYNNHKDLPNSREQNKIYARHQN
>Mature_360_residues
TLKKIAIYGGTFNPVHKAHLEIAQKAIEFLNLDKLFFVPNYINPLKKQKKNIIDPKFRYEMLKLVQIEKTDVCDFEIKAK
KVSYTIDTINFFEKKYPNTKIFLIIGSDNLRNFKLWKNYKEILEKVQLVVFTRKNYPDLRQLKRYNGLILPTKLPNFSSS
QIRKGEFSGLDSKVRAYIGKNFLYAEEILTSFLGYSKRLIHSKNTAKLANEYAKINNLDSKLAYYAGLFHDLTKK*TKDQ
HLEFLRSQKISVKNLQEYQLHQLSASI*LKNVYCLPFEQIIYAISCHTSLCFEMSLFDKIVYVADKLARGRRFLGVQKLR
KLARQDLEKAFQKLVEIAYNNHKDLPNSREQNKIYARHQN

Specific function: Catalyzes The Reversible Adenylation Of Nicotinate Mononucleotide (Namn) To Nicotinic Acid Adenine Dinucleotide (Naad). [C]

COG id: COG1057

COG function: function code H; Nicotinic acid mononucleotide adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1786858, Length=136, Percent_Identity=31.6176470588235, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005249
- InterPro:   IPR004820
- InterPro:   IPR003607
- InterPro:   IPR005248
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2 [H]

EC number: 2.7.7.18

Molecular weight: Translated: 42181; Mature: 42050

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKKIAIYGGTFNPVHKAHLEIAQKAIEFLNLDKLFFVPNYINPLKKQKKNIIDPKFRY
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHEECCHHHHHHHHHHHCCCCHHHHH
EMLKLVQIEKTDVCDFEIKAKKVSYTIDTINFFEKKYPNTKIFLIIGSDNLRNFKLWKNY
HHHHHHHHCCCCCCCEEEEEEEEEEEHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
KEILEKVQLVVFTRKNYPDLRQLKRYNGLILPTKLPNFSSSQIRKGEFSGLDSKVRAYIG
HHHHHHHHEEEEECCCCHHHHHHHHHCCEEEECCCCCCCCHHCCCCCCCCCHHHHHHHHC
KNFLYAEEILTSFLGYSKRLIHSKNTAKLANEYAKINNLDSKLAYYAGLFHDLTKKTKDQ
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
HLEFLRSQKISVKNLQEYQLHQLSASILKNVYCLPFEQIIYAISCHTSLCFEMSLFDKIV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YVADKLARGRRFLGVQKLRKLARQDLEKAFQKLVEIAYNNHKDLPNSREQNKIYARHQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCC
>Mature Secondary Structure 
TLKKIAIYGGTFNPVHKAHLEIAQKAIEFLNLDKLFFVPNYINPLKKQKKNIIDPKFRY
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHEECCHHHHHHHHHHHCCCCHHHHH
EMLKLVQIEKTDVCDFEIKAKKVSYTIDTINFFEKKYPNTKIFLIIGSDNLRNFKLWKNY
HHHHHHHHCCCCCCCEEEEEEEEEEEHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
KEILEKVQLVVFTRKNYPDLRQLKRYNGLILPTKLPNFSSSQIRKGEFSGLDSKVRAYIG
HHHHHHHHEEEEECCCCHHHHHHHHHCCEEEECCCCCCCCHHCCCCCCCCCHHHHHHHHC
KNFLYAEEILTSFLGYSKRLIHSKNTAKLANEYAKINNLDSKLAYYAGLFHDLTKKTKDQ
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
HLEFLRSQKISVKNLQEYQLHQLSASILKNVYCLPFEQIIYAISCHTSLCFEMSLFDKIV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YVADKLARGRRFLGVQKLRKLARQDLEKAFQKLVEIAYNNHKDLPNSREQNKIYARHQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: KCl; NH4Cl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1.1 {diphosphate}} 0.37 {NAD+}} 0.0045 {deamido-NAD+}} 0.5 {ATP}} 0.08 {nicotinate} 0.03 {nicotinate} 0.2 {nicotinamide} [C]

Substrates: ATP; nicotinate ribonucleotide

Specific reaction: ATP + nicotinate ribonucleotide = diphosphate + deamido-NAD+

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NAD+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633; 10954595 [H]