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The map label for this gene is pmbA [H]

Identifier: 161525392

GI number: 161525392

Start: 2438195

End: 2439607

Strand: Reverse

Name: pmbA [H]

Synonym: Bmul_2222

Alternate gene names: 161525392

Gene position: 2439607-2438195 (Counterclockwise)

Preceding gene: 161525395

Following gene: 161525389

Centisome position: 70.74

GC content: 67.87

Gene sequence:

>1413_bases
TTGACCATGCGGGCGGCCTGCCCGCCACCGGATATCACGACGATGGCAGCCAATCTCGACGTACAAGCGCGCTACTTCCC
GCACACGCAGGATCAGCTCAAAGAAATCGCCTCGGACATTCTTCGCCATGCGAAGGCACTCGGCGCGACCGACGCGGCGA
CCGAAATCTCCGAAGGCGACGGCCTGTCCGTCTCGGTGCGCCGCGGCGAGGTCGAGACGATCGAGCACAATCGCGACAAG
ATGGTCGGCGTGACGGTGTTCATCGGCAAGAAGCGCGGCAACGCGAGCACGTCCGATTTCTCGCCGGCCGCCATCAAGGA
TACGGTCGCCGCGGCCTACAACATCGCGCGCTTCACGGCCGAGGACGACGCGGCCGGCCTCGCCGAAGCCGAGCTGCTCG
AGACCGATCCGCGCGATCTCGATCTCTATCACCCATGGGCGCTGTCGGCCGACGAAGCCGTCGACATCGCGCGCCGCGCG
GAAGACGCCGCGTTCGCGGTCAGCCCGCAGATCCGCAATTCGGAAGGCGCGAGCGTGTCCGCGCAGCATTCGCAGTTCGT
GCTCGCCACGTCGCGCGGCTTCCTCGGCGGCTATCCGTACTCGCGTCACTACATCGCGTGCGCGCCGATCGCGGGCAGCG
GCCGTCACATGCAGCGCGACGACTGGTACACGTCGAAGCGCAGCGCGGCCGATCTCGCCGCGCCGGAAGCGGTGGGCCGC
TATGCCGCGGAGCGCGCGCTCGCGCGCATGGGCGCGCGTCGCCTCGACACGCGCAAGGTGCCCGTGCTGTTCGAAGCACC
GCTCGCGGCCGGTCTGCTCGGCGCGTTCGTACAGGCAGTGAGCGGCGGCGCGCTGTACCGGAAGACGTCGTTCCTCGTCG
ACAGCCTCGGCAAGCCGGTGTTCGCGCCGCACGTGCAGATCGTCGAGGATCCGCACGTGCCGCGCGCGATGGGCAGCGCG
CCGTTCGACGAAGAGGGCGTGCGCACGCGCTCGCGCAGCGTCGTGAAGGACGGCGTCGTGGAAGGCTATTTCCTGTCGAC
GTATTCGGCGCGCAAGCTCGGCACGCAGACCACCGGCAACGCCGGCGGCTCGCACAACCTCGCGCTGCGCAGCTCGAACA
CGCAGCCCGGCGACGATTTCGACGCAATGCTGAAGAAGCTCGGCACGGGCCTGCTGCTCACCGAACTGATGGGGCAGGGC
GTGAACTACGTGACCGGCGATTATTCGCGCGGCGCGGCGGGCTTCTGGGTCGAGAACGGTGTGATCCAGTATCCGGTCGA
GGAGATCACGGTCGCGAGCACGCTGCAGGAAATGTTCCGGCACATCGTCGCGATCGGCGCGGATTCGATCGTGCGCGGCA
CGAAGGAAACCGGCTCGGTGCTGATCGAGCAGATGACGATCGCCGGCCAGTAA

Upstream 100 bases:

>100_bases
GTTTTGCGGTTCATGGGCGGCATTGTATCGCGGCGCGCATGCGCCCAGCTTGTCGGTATCCGGCCCCGGACCTTGCTATG
ATCGCGGGATACGCAACATT

Downstream 100 bases:

>100_bases
GCACGGCACTGCGCCGCGTCGGCGGCGCGCTCGGACGAAAAAAAACCGATCGGCGCGAGCCGGTCGGTTTTTTTCATCGG
GCGGTACGTATCAGCCGCGC

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTMRAACPPPDITTMAANLDVQARYFPHTQDQLKEIASDILRHAKALGATDAATEISEGDGLSVSVRRGEVETIEHNRDK
MVGVTVFIGKKRGNASTSDFSPAAIKDTVAAAYNIARFTAEDDAAGLAEAELLETDPRDLDLYHPWALSADEAVDIARRA
EDAAFAVSPQIRNSEGASVSAQHSQFVLATSRGFLGGYPYSRHYIACAPIAGSGRHMQRDDWYTSKRSAADLAAPEAVGR
YAAERALARMGARRLDTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKPVFAPHVQIVEDPHVPRAMGSA
PFDEEGVRTRSRSVVKDGVVEGYFLSTYSARKLGTQTTGNAGGSHNLALRSSNTQPGDDFDAMLKKLGTGLLLTELMGQG
VNYVTGDYSRGAAGFWVENGVIQYPVEEITVASTLQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ

Sequences:

>Translated_470_residues
MTMRAACPPPDITTMAANLDVQARYFPHTQDQLKEIASDILRHAKALGATDAATEISEGDGLSVSVRRGEVETIEHNRDK
MVGVTVFIGKKRGNASTSDFSPAAIKDTVAAAYNIARFTAEDDAAGLAEAELLETDPRDLDLYHPWALSADEAVDIARRA
EDAAFAVSPQIRNSEGASVSAQHSQFVLATSRGFLGGYPYSRHYIACAPIAGSGRHMQRDDWYTSKRSAADLAAPEAVGR
YAAERALARMGARRLDTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKPVFAPHVQIVEDPHVPRAMGSA
PFDEEGVRTRSRSVVKDGVVEGYFLSTYSARKLGTQTTGNAGGSHNLALRSSNTQPGDDFDAMLKKLGTGLLLTELMGQG
VNYVTGDYSRGAAGFWVENGVIQYPVEEITVASTLQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ
>Mature_469_residues
TMRAACPPPDITTMAANLDVQARYFPHTQDQLKEIASDILRHAKALGATDAATEISEGDGLSVSVRRGEVETIEHNRDKM
VGVTVFIGKKRGNASTSDFSPAAIKDTVAAAYNIARFTAEDDAAGLAEAELLETDPRDLDLYHPWALSADEAVDIARRAE
DAAFAVSPQIRNSEGASVSAQHSQFVLATSRGFLGGYPYSRHYIACAPIAGSGRHMQRDDWYTSKRSAADLAAPEAVGRY
AAERALARMGARRLDTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKPVFAPHVQIVEDPHVPRAMGSAP
FDEEGVRTRSRSVVKDGVVEGYFLSTYSARKLGTQTTGNAGGSHNLALRSSNTQPGDDFDAMLKKLGTGLLLTELMGQGV
NYVTGDYSRGAAGFWVENGVIQYPVEEITVASTLQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=439, Percent_Identity=48.0637813211845, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1789640, Length=394, Percent_Identity=23.0964467005076, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 50310; Mature: 50179

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMRAACPPPDITTMAANLDVQARYFPHTQDQLKEIASDILRHAKALGATDAATEISEGD
CCCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
GLSVSVRRGEVETIEHNRDKMVGVTVFIGKKRGNASTSDFSPAAIKDTVAAAYNIARFTA
CCEEEEECCCEEEHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEECC
EDDAAGLAEAELLETDPRDLDLYHPWALSADEAVDIARRAEDAAFAVSPQIRNSEGASVS
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCEEE
AQHSQFVLATSRGFLGGYPYSRHYIACAPIAGSGRHMQRDDWYTSKRSAADLAAPEAVGR
CCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHH
YAAERALARMGARRLDTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKPV
HHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
FAPHVQIVEDPHVPRAMGSAPFDEEGVRTRSRSVVKDGVVEGYFLSTYSARKLGTQTTGN
CCCCEEEECCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCC
AGGSHNLALRSSNTQPGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAGFWVENG
CCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCEEEECC
VIQYPVEEITVASTLQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ
EEECCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TMRAACPPPDITTMAANLDVQARYFPHTQDQLKEIASDILRHAKALGATDAATEISEGD
CCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
GLSVSVRRGEVETIEHNRDKMVGVTVFIGKKRGNASTSDFSPAAIKDTVAAAYNIARFTA
CCEEEEECCCEEEHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEECC
EDDAAGLAEAELLETDPRDLDLYHPWALSADEAVDIARRAEDAAFAVSPQIRNSEGASVS
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCEEE
AQHSQFVLATSRGFLGGYPYSRHYIACAPIAGSGRHMQRDDWYTSKRSAADLAAPEAVGR
CCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHH
YAAERALARMGARRLDTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKPV
HHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
FAPHVQIVEDPHVPRAMGSAPFDEEGVRTRSRSVVKDGVVEGYFLSTYSARKLGTQTTGN
CCCCEEEECCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCC
AGGSHNLALRSSNTQPGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAGFWVENG
CCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCEEEECC
VIQYPVEEITVASTLQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ
EEECCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]