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The map label for this gene is rpoE [H]

Identifier: 161525347

GI number: 161525347

Start: 2394218

End: 2394817

Strand: Reverse

Name: rpoE [H]

Synonym: Bmul_2177

Alternate gene names: 161525347

Gene position: 2394817-2394218 (Counterclockwise)

Preceding gene: 161525348

Following gene: 161525346

Centisome position: 69.45

GC content: 61.17

Gene sequence:

>600_bases
GTGAGTGAAAAAGAAATCGATCAGGTTCTGGTCGAGCGCGTTCAGAAAGGCGACAAGGCAGCGTTCGAACTCCTGGTCTC
CAAATACCACCGCAAGATCATTCGGCTGATCTCGCGCCTCGTGCGGGACCCCGCCGAGGTCGAGGATGTGGCGCAGGACG
CGTTCATCAAGGCGTATCGGGCGTTGCCGCAGTTTCGCGGCGAGTCCGCGTTCTACACGTGGTTGTACCGGATTGCCGTC
AATACGGCCAAGAACTACCTTGCGACGCAGGGCCGCCGGGCGCCGACGTCGACCGAGGCCGATGCCGAGGAGGCGGAAAC
TTTCTCCGACGCAGACCAACTAAGGGATATCAACACGCCTGAGTCGATGTTGATGAGCAAGCAGATTGCCGAAACGGTCA
ACACGGCGATGGCCGCGCTGCCCGAGGAGTTGCGCCAGGCCATCACGCTGCGCGAGATCGAAGGTCTCAGCTACGAGGAA
ATCGCGGAAATGATGGGCTGTCCGATCGGCACGGTCCGGTCGCGGATTTTCCGGGCGCGCGAGGCAATCGCTGCGAAATT
GCGTCCGCTGCTCGATACGCCGGAAGGCAAGCGCTGGTAA

Upstream 100 bases:

>100_bases
ACGGGCTGTTACGACGCGTAACCAGCGGCGCGCGCAGCGGCCGGCGCGGGAACGCTACAATAGCGCTCCGCGTTGCATCC
CTAGTTAACGGATTTGTCAG

Downstream 100 bases:

>100_bases
GCGCCGCCGACGGGCGCAACGACGCGGGTCGGGGTCTAGTTACGGATAGAGTCGTGAAGTCACGACGGGGTGTGCAAGAT
GGGGAGCATCATGGGGTCGG

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSEKEIDQVLVERVQKGDKAAFELLVSKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAV
NTAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTPESMLMSKQIAETVNTAMAALPEELRQAITLREIEGLSYEE
IAEMMGCPIGTVRSRIFRAREAIAAKLRPLLDTPEGKRW

Sequences:

>Translated_199_residues
MSEKEIDQVLVERVQKGDKAAFELLVSKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAV
NTAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTPESMLMSKQIAETVNTAMAALPEELRQAITLREIEGLSYEE
IAEMMGCPIGTVRSRIFRAREAIAAKLRPLLDTPEGKRW
>Mature_198_residues
SEKEIDQVLVERVQKGDKAAFELLVSKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAVN
TAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTPESMLMSKQIAETVNTAMAALPEELRQAITLREIEGLSYEEI
AEMMGCPIGTVRSRIFRAREAIAAKLRPLLDTPEGKRW

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=191, Percent_Identity=61.7801047120419, Blast_Score=239, Evalue=1e-64,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22610; Mature: 22479

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKEIDQVLVERVQKGDKAAFELLVSKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYR
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPQFRGESAFYTWLYRIAVNTAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCH
ESMLMSKQIAETVNTAMAALPEELRQAITLREIEGLSYEEIAEMMGCPIGTVRSRIFRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
EAIAAKLRPLLDTPEGKRW
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEKEIDQVLVERVQKGDKAAFELLVSKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYR
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPQFRGESAFYTWLYRIAVNTAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCH
ESMLMSKQIAETVNTAMAALPEELRQAITLREIEGLSYEEIAEMMGCPIGTVRSRIFRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
EAIAAKLRPLLDTPEGKRW
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]