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The map label for this gene is 161525303

Identifier: 161525303

GI number: 161525303

Start: 2346274

End: 2347695

Strand: Reverse

Name: 161525303

Synonym: Bmul_2133

Alternate gene names: NA

Gene position: 2347695-2346274 (Counterclockwise)

Preceding gene: 161525304

Following gene: 161525302

Centisome position: 68.08

GC content: 61.81

Gene sequence:

>1422_bases
ATGAAAACGCTGTTCTTGCAGGCCCCGTCCTACGACGGCTTCGACGGCGGAGCCGGCTCGCGCTATCAGGCCAAGCGTGA
AATCCGTTCCTTCTGGTATCCGACCTGGCTCGCGCAGCCGGCCGCGCTCGTGCCGGGCAGCCGCGTCGTCGATGCGCCGG
CCGACGGCCTGTCGGTCGAGGAAACGCTGAAGATCGCGAACGACTACGACCTCGTGATCATCCATACGAGCACGCCGTCG
TTCCCGACCGACGCGATGTTCGCGCAGGACCTGAAGAAGATGAAGCCGTCGATGCTGGTCGGCATGGTGGGTGCGAAGGT
GATGGTCGATCCGCACAACTCGCTGACGGCGAGCGACGCGATCGACTTCGTCTGCCGCGAGGAATTCGACTACACCTGCA
AGGAACTCGCCGAAGGCAAGCCGTTCGCCGAGATCAAGGGCTTGAGCTGGCGCGCGAAGGACGGCTCGATCGAGCATAAC
GAAGCGCGTCCGATCCTCGAGAACATGGACGAACTGCCGTTCGTCGCGCCCGTCTACAAGCGAGATCTGAAGATCGAGAA
CTACTTCATCGGCTATCTGAACTATCCGTACGTGTCGATCTACACGGGCCGCGGCTGCAAGTCGCGCTGCACGTTCTGCC
TGTGGCCGCAGACGGTCAGCGGCCACCGCTATCGCACGCGCTCGGTCGAGAACGTGCTCGCGGAAGCGAAGTGGATCCGC
GACAACATGCCGGAAGTGAAGGAACTGATGTTCGACGACGACACCTTCACCGACGATCTGCCGCGCGCGGAAGCGATCGC
CATCGGCCTCGGCAAGCTCGGCATCACGTGGTCGTGCAACGCGAAGGCGAACGTGCCGTACAAGACGCTGAAGGTGATGA
AGGAAAACGGGCTGCGCCTGCTGCTCGTCGGCTTCGAATCCGGCGACGACCAGATCCTCGTGAACATCAAGAAGGGCGTG
CGCACCGATTTCGCGCGCCGCTTCAGCGCGGACTGCAAGAAGCTCGGCATCAAGATCCACGGCACCTTCATCCTCGGCCT
GCCGGGCGAGACGAAAGAGACGATCAAGAAGACGATCCAGTACGCGAAGGAAATCAATCCGCACACGATCCAGGTGTCGC
TCGCCGCGCCGTATCCGGGCACGACGCTCTACAAGCAGGCCATCGAAAACGGCTGGATGGAAGAGAACAAGACGATCAAC
CTCGTCAGCAAGGAAGGCGTGCAGCTCGCCGCGATCGGCTATCCGCACCTGTCGCGCGACGAGATCTATCACCATCTCGA
GCAGTTCTATCGCGAGTTCTACTTCCGTCCGTCGAAGATCTGGGAGATCGTGCGCGAGATGCTGACGAGCTGGGACATGA
TGAAGCGCCGTCTGCGCGAGGGCGTCGAATTCTTCCGTTTCCTGCGCGCGCACGAGGCCTGA

Upstream 100 bases:

>100_bases
GCGCGACATCACGGAGTAGCGGGCAGCCGGTACGGCGGCCCGCCGTTTTTTTGACTGAATCGTTGAGACGAATCGAACTA
TGCAGGCTACCGGAGCATTC

Downstream 100 bases:

>100_bases
TTCGTGACGACGCAGCGGGCGGCGCGGGCGCTCATCTTCACCGCGGACGACTTCGGGCTGCACGAGCGTGTGAATGCGGC
GGTCGAGCGCGCGCATCGCG

Product: hopanoid biosynthesis associated radical SAM protein HpnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKTLFLQAPSYDGFDGGAGSRYQAKREIRSFWYPTWLAQPAALVPGSRVVDAPADGLSVEETLKIANDYDLVIIHTSTPS
FPTDAMFAQDLKKMKPSMLVGMVGAKVMVDPHNSLTASDAIDFVCREEFDYTCKELAEGKPFAEIKGLSWRAKDGSIEHN
EARPILENMDELPFVAPVYKRDLKIENYFIGYLNYPYVSIYTGRGCKSRCTFCLWPQTVSGHRYRTRSVENVLAEAKWIR
DNMPEVKELMFDDDTFTDDLPRAEAIAIGLGKLGITWSCNAKANVPYKTLKVMKENGLRLLLVGFESGDDQILVNIKKGV
RTDFARRFSADCKKLGIKIHGTFILGLPGETKETIKKTIQYAKEINPHTIQVSLAAPYPGTTLYKQAIENGWMEENKTIN
LVSKEGVQLAAIGYPHLSRDEIYHHLEQFYREFYFRPSKIWEIVREMLTSWDMMKRRLREGVEFFRFLRAHEA

Sequences:

>Translated_473_residues
MKTLFLQAPSYDGFDGGAGSRYQAKREIRSFWYPTWLAQPAALVPGSRVVDAPADGLSVEETLKIANDYDLVIIHTSTPS
FPTDAMFAQDLKKMKPSMLVGMVGAKVMVDPHNSLTASDAIDFVCREEFDYTCKELAEGKPFAEIKGLSWRAKDGSIEHN
EARPILENMDELPFVAPVYKRDLKIENYFIGYLNYPYVSIYTGRGCKSRCTFCLWPQTVSGHRYRTRSVENVLAEAKWIR
DNMPEVKELMFDDDTFTDDLPRAEAIAIGLGKLGITWSCNAKANVPYKTLKVMKENGLRLLLVGFESGDDQILVNIKKGV
RTDFARRFSADCKKLGIKIHGTFILGLPGETKETIKKTIQYAKEINPHTIQVSLAAPYPGTTLYKQAIENGWMEENKTIN
LVSKEGVQLAAIGYPHLSRDEIYHHLEQFYREFYFRPSKIWEIVREMLTSWDMMKRRLREGVEFFRFLRAHEA
>Mature_473_residues
MKTLFLQAPSYDGFDGGAGSRYQAKREIRSFWYPTWLAQPAALVPGSRVVDAPADGLSVEETLKIANDYDLVIIHTSTPS
FPTDAMFAQDLKKMKPSMLVGMVGAKVMVDPHNSLTASDAIDFVCREEFDYTCKELAEGKPFAEIKGLSWRAKDGSIEHN
EARPILENMDELPFVAPVYKRDLKIENYFIGYLNYPYVSIYTGRGCKSRCTFCLWPQTVSGHRYRTRSVENVLAEAKWIR
DNMPEVKELMFDDDTFTDDLPRAEAIAIGLGKLGITWSCNAKANVPYKTLKVMKENGLRLLLVGFESGDDQILVNIKKGV
RTDFARRFSADCKKLGIKIHGTFILGLPGETKETIKKTIQYAKEINPHTIQVSLAAPYPGTTLYKQAIENGWMEENKTIN
LVSKEGVQLAAIGYPHLSRDEIYHHLEQFYREFYFRPSKIWEIVREMLTSWDMMKRRLREGVEFFRFLRAHEA

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53969; Mature: 53969

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLFLQAPSYDGFDGGAGSRYQAKREIRSFWYPTWLAQPAALVPGSRVVDAPADGLSVE
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCEEECCCCCCCCHH
ETLKIANDYDLVIIHTSTPSFPTDAMFAQDLKKMKPSMLVGMVGAKVMVDPHNSLTASDA
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHCCEEEECCCCCCCHHHH
IDFVCREEFDYTCKELAEGKPFAEIKGLSWRAKDGSIEHNEARPILENMDELPFVAPVYK
HHHHHHHCCCHHHHHHHCCCCCHHCCCCEEECCCCCCCCCCCCCHHHCHHHCCCCCHHHH
RDLKIENYFIGYLNYPYVSIYTGRGCKSRCTFCLWPQTVSGHRYRTRSVENVLAEAKWIR
HCCEEEEEEEEEECCCEEEEEECCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
DNMPEVKELMFDDDTFTDDLPRAEAIAIGLGKLGITWSCNAKANVPYKTLKVMKENGLRL
CCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHHCCCEE
LLVGFESGDDQILVNIKKGVRTDFARRFSADCKKLGIKIHGTFILGLPGETKETIKKTIQ
EEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHHHHHHH
YAKEINPHTIQVSLAAPYPGTTLYKQAIENGWMEENKTINLVSKEGVQLAAIGYPHLSRD
HHHHCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEECCCCCCHH
EIYHHLEQFYREFYFRPSKIWEIVREMLTSWDMMKRRLREGVEFFRFLRAHEA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTLFLQAPSYDGFDGGAGSRYQAKREIRSFWYPTWLAQPAALVPGSRVVDAPADGLSVE
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCEEECCCCCCCCHH
ETLKIANDYDLVIIHTSTPSFPTDAMFAQDLKKMKPSMLVGMVGAKVMVDPHNSLTASDA
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHCCEEEECCCCCCCHHHH
IDFVCREEFDYTCKELAEGKPFAEIKGLSWRAKDGSIEHNEARPILENMDELPFVAPVYK
HHHHHHHCCCHHHHHHHCCCCCHHCCCCEEECCCCCCCCCCCCCHHHCHHHCCCCCHHHH
RDLKIENYFIGYLNYPYVSIYTGRGCKSRCTFCLWPQTVSGHRYRTRSVENVLAEAKWIR
HCCEEEEEEEEEECCCEEEEEECCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
DNMPEVKELMFDDDTFTDDLPRAEAIAIGLGKLGITWSCNAKANVPYKTLKVMKENGLRL
CCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHHCCCEE
LLVGFESGDDQILVNIKKGVRTDFARRFSADCKKLGIKIHGTFILGLPGETKETIKKTIQ
EEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHHHHHHH
YAKEINPHTIQVSLAAPYPGTTLYKQAIENGWMEENKTINLVSKEGVQLAAIGYPHLSRD
HHHHCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEECCCCCCHH
EIYHHLEQFYREFYFRPSKIWEIVREMLTSWDMMKRRLREGVEFFRFLRAHEA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]