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The map label for this gene is amtB [C]

Identifier: 161525271

GI number: 161525271

Start: 2304526

End: 2305719

Strand: Direct

Name: amtB [C]

Synonym: Bmul_2101

Alternate gene names: 161525271

Gene position: 2304526-2305719 (Clockwise)

Preceding gene: 161525270

Following gene: 161525272

Centisome position: 66.83

GC content: 67.67

Gene sequence:

>1194_bases
ATGGACGGTATGAAATCCGGCGTCGACACACTGTTCCTGTTGATCGGCGCCGTCATGGTGCTCGCGATGCACGCGGGCTT
CGCGTTTCTCGAACTCGGCACGGTGCGCAAGAAGAATCAGGTGAATGCGCTCGTGAAGATCCTCGTCGACTTCTCGGTGT
CGACGCTCGCGTATTTCTTCATCGGCTATACGATCGCGTACGGCGTCGAGTTCTTCGACGACATCGGCACGTTGTCGCAG
CACAGCGGCTATGCGCTCGTGCGGTTCTTCTTTCTGCTGACCTTCGCGGCCGCGATACCGGCGATCGTCTCCGGCGGCAT
CGCCGAGCGCGCGAAATTCAATCCGCAGCTCGTCGCGACGCTGATCATCGTCGGCTTCATCTATCCGTTCTTCGAAGGCA
TCGCCTGGAACGAGCGCTACGGCGTGCAGGCCTGGCTCGCGCACGCGTTCGGTGCACCGTTCCACGATTTCGCGGGCTCG
GTCGTCGTGCACGCGTTCGGCGGCTGGGTCGCGCTGCCGGCCGTGCTGCTGCTCGGCGCGCGCCACGGCCGCTACGCGAA
GGACGGCCGCATCGCCGCGCATCCGCCGTCGAACATTCCGTTTCTCGCGCTCGGCGCGTGGGTGCTCGCGGTCGGCTGGT
TCGGCTTCAACGTGATGAGCGCGCAGACGCTCGACAAGATCAGCGGCCTCGTCGCCGTGAACTCGCTGATGGCGATGGTC
GGCGGCACGCTCGCCGCGTGGCTCGCGGGACGCAACGATCCGGGCTTCACGTACAACGGCCCGCTCGCCGGCCTCGTCGC
TGTGTGCGCGGGCTCCGACGTGATGCACCCGATCGGTGCGCTCGTGACGGGCGCGGCCGCCGGCGCGCTGTTCGTCGCGA
TGTTCACGTGCGTGCAGAACAAGTGGCGCATCGACGACGTGCTCGGCGTGTGGCCGCTGCACGGCATGTGCGGCGCGCTC
GGCGGCATCGCGGCGGGCATCTTCGGGCTGCCGGCGCTCGGCGGCCTCGGCGGCGTCGCGTTCATGTCGCAGCTGATCGG
CACGGTCGGCGGCATCGCGATCGCCAGTGTCGGCGGCGCGCTCGTCTACGGCGCGCTGAAAGCGACCGTCGGCCTGCGGC
TCGATCGCGAAGCGGAGTTCGACGGCGCGGATCTGTCGATCCATCGGATCACGGCGACGCCCGAACGCGACTGA

Upstream 100 bases:

>100_bases
CCGCGCCGCGCGGCGCTTCGCACCAACGCCACGCATGCACTCCCCAACTTGGCTCGATACTTGCATTCGTTGCCCGTTTT
TTGAGCAAGGAAGCCTCCGC

Downstream 100 bases:

>100_bases
CGCCACGCGCGCCGCACGCGTTCGCAGCGGTGCGCCGACGCACGCTCGATACGCATTACGTTTTCAATTCAAAAAACTTT
CATCGAGCGTCCCTACACTT

Product: Rh family protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MDGMKSGVDTLFLLIGAVMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFSVSTLAYFFIGYTIAYGVEFFDDIGTLSQ
HSGYALVRFFFLLTFAAAIPAIVSGGIAERAKFNPQLVATLIIVGFIYPFFEGIAWNERYGVQAWLAHAFGAPFHDFAGS
VVVHAFGGWVALPAVLLLGARHGRYAKDGRIAAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTLDKISGLVAVNSLMAMV
GGTLAAWLAGRNDPGFTYNGPLAGLVAVCAGSDVMHPIGALVTGAAAGALFVAMFTCVQNKWRIDDVLGVWPLHGMCGAL
GGIAAGIFGLPALGGLGGVAFMSQLIGTVGGIAIASVGGALVYGALKATVGLRLDREAEFDGADLSIHRITATPERD

Sequences:

>Translated_397_residues
MDGMKSGVDTLFLLIGAVMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFSVSTLAYFFIGYTIAYGVEFFDDIGTLSQ
HSGYALVRFFFLLTFAAAIPAIVSGGIAERAKFNPQLVATLIIVGFIYPFFEGIAWNERYGVQAWLAHAFGAPFHDFAGS
VVVHAFGGWVALPAVLLLGARHGRYAKDGRIAAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTLDKISGLVAVNSLMAMV
GGTLAAWLAGRNDPGFTYNGPLAGLVAVCAGSDVMHPIGALVTGAAAGALFVAMFTCVQNKWRIDDVLGVWPLHGMCGAL
GGIAAGIFGLPALGGLGGVAFMSQLIGTVGGIAIASVGGALVYGALKATVGLRLDREAEFDGADLSIHRITATPERD
>Mature_397_residues
MDGMKSGVDTLFLLIGAVMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFSVSTLAYFFIGYTIAYGVEFFDDIGTLSQ
HSGYALVRFFFLLTFAAAIPAIVSGGIAERAKFNPQLVATLIIVGFIYPFFEGIAWNERYGVQAWLAHAFGAPFHDFAGS
VVVHAFGGWVALPAVLLLGARHGRYAKDGRIAAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTLDKISGLVAVNSLMAMV
GGTLAAWLAGRNDPGFTYNGPLAGLVAVCAGSDVMHPIGALVTGAAAGALFVAMFTCVQNKWRIDDVLGVWPLHGMCGAL
GGIAAGIFGLPALGGLGGVAFMSQLIGTVGGIAIASVGGALVYGALKATVGLRLDREAEFDGADLSIHRITATPERD

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=405, Percent_Identity=31.358024691358, Blast_Score=137, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17550180, Length=443, Percent_Identity=29.3453724604966, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17550182, Length=434, Percent_Identity=29.0322580645161, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI32564042, Length=351, Percent_Identity=29.0598290598291, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI133931100, Length=439, Percent_Identity=28.246013667426, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325396, Length=354, Percent_Identity=24.8587570621469, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321559, Length=415, Percent_Identity=24.3373493975904, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324187, Length=333, Percent_Identity=27.6276276276276, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI161078314, Length=415, Percent_Identity=28.6746987951807, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 41485; Mature: 41485

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCEECCCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCEECCCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]