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The map label for this gene is thiC [H]

Identifier: 161525251

GI number: 161525251

Start: 2277575

End: 2279506

Strand: Direct

Name: thiC [H]

Synonym: Bmul_2081

Alternate gene names: 161525251

Gene position: 2277575-2279506 (Clockwise)

Preceding gene: 161525249

Following gene: 161525252

Centisome position: 66.05

GC content: 64.86

Gene sequence:

>1932_bases
ATGAACGCCAATCCGAAGTTTCTGTCTGCCGACGCCCACGTCGACGCTGCTGCCGTCGCGCCGCTGCCCAATTCGCGGAA
AGTCTATGTGACGGGTTCGCAGCCCGACATCCGCGTGCCGATGCGCGAGATCACGCAGGCCGACACGCCGACCGGCTTCG
GCGGCGAAAAGAATCCCCCGATCTACGTCTACGACACGTCGGGCCCGTACACCGATCCGGACGCCAAGATCGACATCCGC
GCGGGCCTGCCGGCGCTGCGTCAGCGCTGGATCGAAGCACGCGGCGACACCGAAACGCTGCCCGGCCTGTCGAGCCAGTA
CGGCCGCGAGCGCGCGGCCGATCCGGCCACGGCCGACCTGCGCTTCCCGGGCCTGCACCGCAATCCGCGCCGCGCGCGCG
CCGGCAAGAACGTGACGCAGATGCACTATGCGCGTCAGGGGATCATCACGCCGGAGATGGAGTTCATCGCGATCCGCGAA
AACCAGCGTCGCGCCGAATACCTCGAGAGCCTGAAGGCGAGCGGCCCGAACGGCGCGAAGCTCGCCGCGATGATGGGCCG
CCAGCATCCGGGGCAGGCGTTCGGCGCCGCGGCATTCGGCGGCAACGCGCCGAGCGAGATCACGCCGGAATTCGTGCGCG
ACGAAGTGGCGCGCGGCCGCGCGATCATCCCGGCCAACATCAATCACCCGGAATCGGAGCCGATGATCATCGGCCGCAAC
TTCCTCGTGAAGATCAACGCGAACATCGGCAACTCGGCCGTCACGTCGTCGATCGGCGAGGAAGTCGACAAGATGACGTG
GGCGATCCGCTGGGGCGGCGATACGGTGATGGATCTGTCGACCGGCAAGCACATCCATGAAACGCGCGAGTGGATCATCC
GCAACAGCCCGGTGCCGATCGGCACGGTGCCGATCTATCAGGCGCTCGAGAAGGTCAACGGCAAGGCCGAGGAACTGACC
TGGGAAATCTTCCGCGACACGCTGATCGAGCAGGCCGAGCAAGGCGTCGACTACTTCACGATCCACGCCGGCGTGCGCCT
GCAGTACGTGCCGCTCACCGCGAACCGGATGACGGGCATCGTGTCGCGCGGCGGCTCGATCATGGCGAAGTGGTGTCTCG
CGCATCATAAGGAAAGCTTCCTCTACGAACACTTCGAAGAGATCTGCGAGATCATGAAGGCGTACGACGTGAGCTTCTCG
CTCGGCGACGGCCTGCGCCCCGGCTCGATCTACGACGCGAACGACGAAGCGCAGCTCGGCGAGCTGAAGACGCTCGGCGA
GCTCACGCAGATCGCGTGGAAGCACGACGTCCAGGTGATGATCGAAGGTCCGGGCCACGTACCGATGCAGCTGATCAAGG
AAAACATGGATCTGCAGCTCGACTGGTGCAAGGAAGCGCCCTTCTATACGCTCGGGCCGCTGACGACCGACATCGCGCCG
GGCTACGACCACATCACGTCGGGCATCGGCGCCGCGATGATCGGCTGGTTCGGCACCGCGATGCTCTGCTACGTGACGCC
GAAGGAACACCTCGGTCTGCCGAACAAGGACGACGTGAAGGAAGGCATCATCACGTACAAGCTCGCCGCGCATGCCGCCG
ATCTCGCGAAGGGTCATCCGGGCGCGCAGGTGCGCGACAACGCGTTGTCGAAGGCGCGCTTCGAGTTCCGCTGGGAAGAC
CAGTTCAACCTCGGCCTGGATCCCGACAAGGCGCGCGAATTCCACGACGAAACGCTGCCGAAGGATTCGGCGAAGGTCGC
GCACTTCTGCTCGATGTGCGGCCCGCACTTCTGCTCGATGAAGATCACGCAGGACGTGCGCGAGTTCGCAGCCCAGCAGG
GCGTGTCCGAAGCCGACGCGCTGCAGAAAGGGATGGAAGTGAAGGCCGTCGAGTTCGTGAAGAGCGGCTCGGAAATCTAT
CACCGTCAGTAA

Upstream 100 bases:

>100_bases
GCCGCCTCACCAAACTCGTCTCCTGCTTAGCTCGAGCCCCCATTCGTCACGGGGCCCGGGTGAACGCCAAGGCGCCCACT
CGGGCCGACAAGGAGATTGC

Downstream 100 bases:

>100_bases
CGCACGGCGCGCCGCGGCGCGCACGTCGTTCGTCGAGCCCGCCTTCGCGCGGGCTTTTTTGCGCGGGCCGCTCGCATCGC
GCGTGCCTGTGCGTACGGAC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MNANPKFLSADAHVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPPIYVYDTSGPYTDPDAKIDIR
AGLPALRQRWIEARGDTETLPGLSSQYGRERAADPATADLRFPGLHRNPRRARAGKNVTQMHYARQGIITPEMEFIAIRE
NQRRAEYLESLKASGPNGAKLAAMMGRQHPGQAFGAAAFGGNAPSEITPEFVRDEVARGRAIIPANINHPESEPMIIGRN
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEELT
WEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAYDVSFS
LGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLTTDIAP
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWED
QFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVREFAAQQGVSEADALQKGMEVKAVEFVKSGSEIY
HRQ

Sequences:

>Translated_643_residues
MNANPKFLSADAHVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPPIYVYDTSGPYTDPDAKIDIR
AGLPALRQRWIEARGDTETLPGLSSQYGRERAADPATADLRFPGLHRNPRRARAGKNVTQMHYARQGIITPEMEFIAIRE
NQRRAEYLESLKASGPNGAKLAAMMGRQHPGQAFGAAAFGGNAPSEITPEFVRDEVARGRAIIPANINHPESEPMIIGRN
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEELT
WEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAYDVSFS
LGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLTTDIAP
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWED
QFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVREFAAQQGVSEADALQKGMEVKAVEFVKSGSEIY
HRQ
>Mature_643_residues
MNANPKFLSADAHVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPPIYVYDTSGPYTDPDAKIDIR
AGLPALRQRWIEARGDTETLPGLSSQYGRERAADPATADLRFPGLHRNPRRARAGKNVTQMHYARQGIITPEMEFIAIRE
NQRRAEYLESLKASGPNGAKLAAMMGRQHPGQAFGAAAFGGNAPSEITPEFVRDEVARGRAIIPANINHPESEPMIIGRN
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEELT
WEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAYDVSFS
LGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLTTDIAP
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWED
QFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVREFAAQQGVSEADALQKGMEVKAVEFVKSGSEIY
HRQ

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=641, Percent_Identity=69.1107644305772, Blast_Score=910, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 71075; Mature: 71075

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNANPKFLSADAHVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPP
CCCCCCEEECCCCCCCEEECCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCC
IYVYDTSGPYTDPDAKIDIRAGLPALRQRWIEARGDTETLPGLSSQYGRERAADPATADL
EEEEECCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHCHHHCCCCCCCCC
RFPGLHRNPRRARAGKNVTQMHYARQGIITPEMEFIAIRENQRRAEYLESLKASGPNGAK
CCCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHH
LAAMMGRQHPGQAFGAAAFGGNAPSEITPEFVRDEVARGRAIIPANINHPESEPMIIGRN
HHHHHCCCCCCCHHCCHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCE
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPI
EEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCC
GTVPIYQALEKVNGKAEELTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGI
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECHHHHHHH
VSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAYDVSFSLGDGLRPGSIYDANDEAQLG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCCHHH
ELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLTTDIAP
HHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCC
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHP
CHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHHEEEHHHHHHHHHHCCCC
GAQVRDNALSKARFEFRWEDQFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSM
CCCHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHEEE
KITQDVREFAAQQGVSEADALQKGMEVKAVEFVKSGSEIYHRQ
HHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCCHHHCCC
>Mature Secondary Structure
MNANPKFLSADAHVDAAAVAPLPNSRKVYVTGSQPDIRVPMREITQADTPTGFGGEKNPP
CCCCCCEEECCCCCCCEEECCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCC
IYVYDTSGPYTDPDAKIDIRAGLPALRQRWIEARGDTETLPGLSSQYGRERAADPATADL
EEEEECCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHCHHHCCCCCCCCC
RFPGLHRNPRRARAGKNVTQMHYARQGIITPEMEFIAIRENQRRAEYLESLKASGPNGAK
CCCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHH
LAAMMGRQHPGQAFGAAAFGGNAPSEITPEFVRDEVARGRAIIPANINHPESEPMIIGRN
HHHHHCCCCCCCHHCCHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCE
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPI
EEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCC
GTVPIYQALEKVNGKAEELTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGI
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECHHHHHHH
VSRGGSIMAKWCLAHHKESFLYEHFEEICEIMKAYDVSFSLGDGLRPGSIYDANDEAQLG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCCHHH
ELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLDWCKEAPFYTLGPLTTDIAP
HHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCC
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHP
CHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHHEEEHHHHHHHHHHCCCC
GAQVRDNALSKARFEFRWEDQFNLGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSM
CCCHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHEEE
KITQDVREFAAQQGVSEADALQKGMEVKAVEFVKSGSEIYHRQ
HHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA