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The map label for this gene is phnV [H]

Identifier: 161525246

GI number: 161525246

Start: 2271098

End: 2271994

Strand: Direct

Name: phnV [H]

Synonym: Bmul_2076

Alternate gene names: 161525246

Gene position: 2271098-2271994 (Clockwise)

Preceding gene: 161525245

Following gene: 161525247

Centisome position: 65.86

GC content: 71.24

Gene sequence:

>897_bases
ATGCCATCGCTTTCCGATTCTTCCGCGCCGCCGCGGGCCGCGTCGTCGGCGCGCGCGGCCGGCCGTTCGCGCCGCGTACC
GCGCCAGGCCGGCGGCCGCGCGATCGCCGCGCTGCAATGGAGCGTCACGCTGCTGCTCTGCGCGTTCCTGATCGTGCCCG
TCGCGATGTCGGTGCTCGCGGGCCTGACCGTCAACTATTTCCGCGGGCTGTCGAGCGGCCTCACGCTGCGCTGGCTCGAA
CAGGTGTGGCAGCAGTATCACGGCTCGATCTGGCTGTCGCTCGCCGTCGCGCTCGCGACGCTCGCGATCGTGCTCGTCGT
CGGCGTGCCGGCCGGCTATGCGCTCGCGCGCAGCCGCAGCCGCGTCGCGCGCGTGATCGAGGAAGCGCTCGTGCTGCCCG
TCGCTCTGCCGGGCCTCGCGTCGGCGCTCGCGCTGCTGATCGTCTACGGCGGCTTCACCGCATTCCGGATGAGCCTGTGG
TTCATCGTCGCCGGCCACGTCGTGTTCACGCTGCCGTTCATGGTGCGCGCGGTCGCGGCCGTCGCGGCGAGCGCGGACCT
GCGCACGCTCGAGGAAGGCGCGGCGAGCCTCGGCGCGTCGTTCGCCACGCGCTTCGCGACGATCGTGCTGCCGAACCTGC
GCCCCGGCATCGTCGCGGGGGCGCTCGCGGTGCTCACGCTATCGATCGGCGAATTCAACCTCACGTGGATGCTGCATACG
CCCGACACGAAGACGCTGCCAGTCGGTCTCGCCGACACCTATGCGTCGCTGCGTCTCGAAGTCGGCAGCGCCTACACGAT
CCTGTTCCTGCTGATGACGCTGCCGCTGCTCGTCGCGATGCAATGGCTCGGCGTCGATCCGTCCGGCACGCGCGCGTCGA
AGCGGCGCGCACGCTGA

Upstream 100 bases:

>100_bases
GACTGTCGATCGTGCTCGGCCTCGTCACGTGGGCCGTGCTCGCGCTCGCGCGCCGGTTCGCCGGTCACACCGCCGCTGCC
GCGGCCTGAGGAGGCATTCG

Downstream 100 bases:

>100_bases
TCTTTCCGTTCCGATGAAACTCGATTCCATTCCCATTACGCTGACCCGCTGCGCGAAGACGTTTCGCGGCACGCGCGTGC
TCGAACCGCTCGACCTGTCG

Product: binding-protein-dependent transport systems inner membrane component

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MPSLSDSSAPPRAASSARAAGRSRRVPRQAGGRAIAALQWSVTLLLCAFLIVPVAMSVLAGLTVNYFRGLSSGLTLRWLE
QVWQQYHGSIWLSLAVALATLAIVLVVGVPAGYALARSRSRVARVIEEALVLPVALPGLASALALLIVYGGFTAFRMSLW
FIVAGHVVFTLPFMVRAVAAVAASADLRTLEEGAASLGASFATRFATIVLPNLRPGIVAGALAVLTLSIGEFNLTWMLHT
PDTKTLPVGLADTYASLRLEVGSAYTILFLLMTLPLLVAMQWLGVDPSGTRASKRRAR

Sequences:

>Translated_298_residues
MPSLSDSSAPPRAASSARAAGRSRRVPRQAGGRAIAALQWSVTLLLCAFLIVPVAMSVLAGLTVNYFRGLSSGLTLRWLE
QVWQQYHGSIWLSLAVALATLAIVLVVGVPAGYALARSRSRVARVIEEALVLPVALPGLASALALLIVYGGFTAFRMSLW
FIVAGHVVFTLPFMVRAVAAVAASADLRTLEEGAASLGASFATRFATIVLPNLRPGIVAGALAVLTLSIGEFNLTWMLHT
PDTKTLPVGLADTYASLRLEVGSAYTILFLLMTLPLLVAMQWLGVDPSGTRASKRRAR
>Mature_297_residues
PSLSDSSAPPRAASSARAAGRSRRVPRQAGGRAIAALQWSVTLLLCAFLIVPVAMSVLAGLTVNYFRGLSSGLTLRWLEQ
VWQQYHGSIWLSLAVALATLAIVLVVGVPAGYALARSRSRVARVIEEALVLPVALPGLASALALLIVYGGFTAFRMSLWF
IVAGHVVFTLPFMVRAVAAVAASADLRTLEEGAASLGASFATRFATIVLPNLRPGIVAGALAVLTLSIGEFNLTWMLHTP
DTKTLPVGLADTYASLRLEVGSAYTILFLLMTLPLLVAMQWLGVDPSGTRASKRRAR

Specific function: Probably part of the phnSTUV complex (TC 3.A.1.11.5) involved in 2-aminoethylphosphonate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=227, Percent_Identity=27.7533039647577, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017661
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31563; Mature: 31432

Theoretical pI: Translated: 11.73; Mature: 11.73

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLSDSSAPPRAASSARAAGRSRRVPRQAGGRAIAALQWSVTLLLCAFLIVPVAMSVLA
CCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
GLTVNYFRGLSSGLTLRWLEQVWQQYHGSIWLSLAVALATLAIVLVVGVPAGYALARSRS
HHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RVARVIEEALVLPVALPGLASALALLIVYGGFTAFRMSLWFIVAGHVVFTLPFMVRAVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAASADLRTLEEGAASLGASFATRFATIVLPNLRPGIVAGALAVLTLSIGEFNLTWMLHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEC
PDTKTLPVGLADTYASLRLEVGSAYTILFLLMTLPLLVAMQWLGVDPSGTRASKRRAR
CCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCC
>Mature Secondary Structure 
PSLSDSSAPPRAASSARAAGRSRRVPRQAGGRAIAALQWSVTLLLCAFLIVPVAMSVLA
CCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
GLTVNYFRGLSSGLTLRWLEQVWQQYHGSIWLSLAVALATLAIVLVVGVPAGYALARSRS
HHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RVARVIEEALVLPVALPGLASALALLIVYGGFTAFRMSLWFIVAGHVVFTLPFMVRAVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAASADLRTLEEGAASLGASFATRFATIVLPNLRPGIVAGALAVLTLSIGEFNLTWMLHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEC
PDTKTLPVGLADTYASLRLEVGSAYTILFLLMTLPLLVAMQWLGVDPSGTRASKRRAR
CCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA