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The map label for this gene is yafC [C]

Identifier: 161525152

GI number: 161525152

Start: 2178456

End: 2179388

Strand: Reverse

Name: yafC [C]

Synonym: Bmul_1980

Alternate gene names: 161525152

Gene position: 2179388-2178456 (Counterclockwise)

Preceding gene: 161525154

Following gene: 161525148

Centisome position: 63.2

GC content: 66.24

Gene sequence:

>933_bases
ATGGAATTGGATTCAACAATGGCCAGCTTCCCGACCGAACATCTGAAGGGCATCGTTCCATTCGTCTGTGTCGCCGACGC
GGGCGGTTTCACCGCTGCGGCCGAGCGGCTGAACCTCACGAATTCGGCGGTGAGCAAGAGTGTCGCCCGGCTCGAACGGC
GGCTCGGCGTCGTGCTGTTCGAGCGGACGACGCGCAAGCTCGTGCTCACGGACGCCGGCCGTGCGTTCTATGAAACCTGC
ACGCGCATCCTGGCGGAGCTTGCGGAGGCGGAGGCCGTGCTCGCCGCGCAACAGCGCGAGCCGGTCGGCACCGTTCGCAT
CGCCTTGCCGGCCTCGTTCGGGCGTCTCTGCGTCGTGCCGGTGCTCGTGCGCTTTTCGGAGCAATATCCGGACGTGCGGC
CGCACCTGTTGTTCACGGACCGCTTCGTCGATCTGGCAGAAGAGGGCATCGATCTCGCCGTGCGGATCGGCGGGCCGCCG
GGTCTGCCGCCGTCGCTCGGTCGGCGTTTTCTCGGGCGCGAACGACTGATCTTCTGCGCGGCGCCGGCCTATCTCGAGAA
AAACGGCATACCGCAAACGGTCGACGACCTGCAGGAACATGCCTGCATCGTCTACGGACGCCCCGACGGAGCGCAGTCGC
TGTGGTCATATGCCGCGGCGGACGGGCAGGTCGAGCAACGCGTCGTGCGGCCACGCATCGTCGCAGGCGACGCCGAGGCA
CAACTCGCATTCGTACGCGCGGGGCTCGGCGTCGCACAGCTGGCGACATGGCTGGTACGGGACGATCTGAAGGCCGGCCG
CCTCGTCGCTATCCTCGACGAAGCCGCAACGGACGGCTTGCCGCTCAGCCTCATATGGCCGGTCGCGCGGCAGTTGACGC
CGAAGATCCATGTGCTGGTGCAGACGCTCGAAGCTCATCTGCATATCGAATGA

Upstream 100 bases:

>100_bases
GACGATCAGTGCTCGATGATTCATCGTGACTCCAGGTTCGTTGGTGATACGAGCGTACTTCAGACCTGGATTCTCATCTT
GGATAGTATTTCGGGGTTTA

Downstream 100 bases:

>100_bases
ACAAGATCGTCCCAAATCGACATGCTGACAATGTCGACAGCCCAGGCTTGATTGCGGAATTGATGACCGAAGGCGTTGAA
GGGCACGCCGTCCTGCGTGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MELDSTMASFPTEHLKGIVPFVCVADAGGFTAAAERLNLTNSAVSKSVARLERRLGVVLFERTTRKLVLTDAGRAFYETC
TRILAELAEAEAVLAAQQREPVGTVRIALPASFGRLCVVPVLVRFSEQYPDVRPHLLFTDRFVDLAEEGIDLAVRIGGPP
GLPPSLGRRFLGRERLIFCAAPAYLEKNGIPQTVDDLQEHACIVYGRPDGAQSLWSYAAADGQVEQRVVRPRIVAGDAEA
QLAFVRAGLGVAQLATWLVRDDLKAGRLVAILDEAATDGLPLSLIWPVARQLTPKIHVLVQTLEAHLHIE

Sequences:

>Translated_310_residues
MELDSTMASFPTEHLKGIVPFVCVADAGGFTAAAERLNLTNSAVSKSVARLERRLGVVLFERTTRKLVLTDAGRAFYETC
TRILAELAEAEAVLAAQQREPVGTVRIALPASFGRLCVVPVLVRFSEQYPDVRPHLLFTDRFVDLAEEGIDLAVRIGGPP
GLPPSLGRRFLGRERLIFCAAPAYLEKNGIPQTVDDLQEHACIVYGRPDGAQSLWSYAAADGQVEQRVVRPRIVAGDAEA
QLAFVRAGLGVAQLATWLVRDDLKAGRLVAILDEAATDGLPLSLIWPVARQLTPKIHVLVQTLEAHLHIE
>Mature_310_residues
MELDSTMASFPTEHLKGIVPFVCVADAGGFTAAAERLNLTNSAVSKSVARLERRLGVVLFERTTRKLVLTDAGRAFYETC
TRILAELAEAEAVLAAQQREPVGTVRIALPASFGRLCVVPVLVRFSEQYPDVRPHLLFTDRFVDLAEEGIDLAVRIGGPP
GLPPSLGRRFLGRERLIFCAAPAYLEKNGIPQTVDDLQEHACIVYGRPDGAQSLWSYAAADGQVEQRVVRPRIVAGDAEA
QLAFVRAGLGVAQLATWLVRDDLKAGRLVAILDEAATDGLPLSLIWPVARQLTPKIHVLVQTLEAHLHIE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=30.7958477508651, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI145693193, Length=283, Percent_Identity=31.095406360424, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=30.0675675675676, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI87081978, Length=268, Percent_Identity=32.8358208955224, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789440, Length=294, Percent_Identity=29.9319727891156, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787128, Length=286, Percent_Identity=28.6713286713287, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787589, Length=304, Percent_Identity=25.6578947368421, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788706, Length=262, Percent_Identity=29.3893129770992, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789173, Length=261, Percent_Identity=29.5019157088123, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788481, Length=257, Percent_Identity=28.4046692607004, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787601, Length=120, Percent_Identity=35.8333333333333, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=27.6094276094276, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=136, Percent_Identity=30.8823529411765, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790011, Length=252, Percent_Identity=26.1904761904762, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786713, Length=275, Percent_Identity=26.9090909090909, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1786448, Length=139, Percent_Identity=29.4964028776978, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082132, Length=301, Percent_Identity=24.2524916943522, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788748, Length=119, Percent_Identity=36.1344537815126, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33742; Mature: 33742

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELDSTMASFPTEHLKGIVPFVCVADAGGFTAAAERLNLTNSAVSKSVARLERRLGVVLF
CCCCHHHHHCCHHHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCEEEE
ERTTRKLVLTDAGRAFYETCTRILAELAEAEAVLAAQQREPVGTVRIALPASFGRLCVVP
ECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
VLVRFSEQYPDVRPHLLFTDRFVDLAEEGIDLAVRIGGPPGLPPSLGRRFLGRERLIFCA
HHHHHHHHCCCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEE
APAYLEKNGIPQTVDDLQEHACIVYGRPDGAQSLWSYAAADGQVEQRVVRPRIVAGDAEA
CCHHHHCCCCCCHHHHHHHCEEEEEECCCCHHHHHHHHHCCCHHHHHHHCCEEEECCCHH
QLAFVRAGLGVAQLATWLVRDDLKAGRLVAILDEAATDGLPLSLIWPVARQLTPKIHVLV
HHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHCHHHHHHH
QTLEAHLHIE
HHHHHHHCCC
>Mature Secondary Structure
MELDSTMASFPTEHLKGIVPFVCVADAGGFTAAAERLNLTNSAVSKSVARLERRLGVVLF
CCCCHHHHHCCHHHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCEEEE
ERTTRKLVLTDAGRAFYETCTRILAELAEAEAVLAAQQREPVGTVRIALPASFGRLCVVP
ECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
VLVRFSEQYPDVRPHLLFTDRFVDLAEEGIDLAVRIGGPPGLPPSLGRRFLGRERLIFCA
HHHHHHHHCCCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEE
APAYLEKNGIPQTVDDLQEHACIVYGRPDGAQSLWSYAAADGQVEQRVVRPRIVAGDAEA
CCHHHHCCCCCCHHHHHHHCEEEEEECCCCHHHHHHHHHCCCHHHHHHHCCEEEECCCHH
QLAFVRAGLGVAQLATWLVRDDLKAGRLVAILDEAATDGLPLSLIWPVARQLTPKIHVLV
HHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHCHHHHHHH
QTLEAHLHIE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]