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The map label for this gene is citN [H]

Identifier: 161525101

GI number: 161525101

Start: 2115492

End: 2116790

Strand: Reverse

Name: citN [H]

Synonym: Bmul_1929

Alternate gene names: 161525101

Gene position: 2116790-2115492 (Counterclockwise)

Preceding gene: 161525103

Following gene: 161525100

Centisome position: 61.38

GC content: 68.21

Gene sequence:

>1299_bases
ATGCTGCCGTTACTCGGGCTCGGCACGATCGTCGTGCTGCTCGCCGCGATTCTGTCCCGCCGCATGTCGCCGCTCGTCGC
GCTGATCGTCGTGCCGATCGTCGCGTCGCTGCTCGGCGGGTTCGGGCTGCAGACCAGCAAGTTCGTGCTCGACGGGCTGA
AGGGCCTCGCGCCCGTGGTCGGCATGTTCGTGTTCGCGATCCTCTATTTCGGGACGATCACCGATGCGGGCACGCTCGAT
CCGATCATCGATCGCATCCTGCGCGCGGTCGGCACGCGGCCGACGCGGATCGTGACGGGCACGACGCTGCTCGCGCTGCT
GATCCATCTCGACGGCTCGGGCGCCGTGTGCTTTCTCGTGACGATCCCGGCCGTGCTGCCGCTCTACGACCGGCTCGGCA
TCGACCGCCGCGTGCTGGCGGCGGCCGTGTCGATGGCGGCCGGCATCAATTTCTTGCCGTGGACGGGGCCGATGATCCGC
GCATCGGCGTCGCTGCATCTGCCCGTGTCGACGCTGTTCAATCCGCTGATTCCGGTCCAGGCGGTCGCCCTCGTGTTCGT
GTTCGGCGTCGCCTACTGGCTCGGCCGGCGCGAGGAGAAGCGGCTCGGCCTGACGCGGCACGACCCGGCGATTGCGCTGC
CCAAGCGCGAGCTGACGGCCGACGAACGCGCGCTGCGCCGGCCGCAGCTGTTCTGGTTCAACATCGTGCTGACGCTCGTC
GTGCTCGGCACGATGGTCGCGATGGGCGAGAAGGTGCCGCCCGCGATCGTGTTCATGGTCGGGCTCTGCATCGCGCTGAC
CGTCAACTATCCCGACGTCGCGATGCAGCGCAAGCGGATCGACGCGCATGCGCGCGCGGCGCTGATGATGGCCGCGATCC
TGCTCGCGGCCGGCGTGTTCACCGGGATCATGCAGGGCAGCGGGATGCTGAAGGCGATGGCGCAGGCCGCGGTCGGCTTC
GTGCCGGCCTCGATGGCCGCGCACATTCCGGTCGTGCTCGGCATCGTGTCGATGCCGCTCAGCATGCTGTTCGATCCCGA
CTCGTTCTATTTCGGCGTGCTGCCGGTGATCGCCGAGGTCGCCGGCCAGCTCGGCGTGCCGGCCGTGAAGGTCGGGCAGG
CCGCGCTGCTCGGCCAGATGACGACCGGTTTCCCGGTCAGTCCGCTCACGCCCGCGACGTTTCTCGTCGTCGGCCTGTGC
GGCATCGAACTGGCCGAGCATCAGAAGTTCACGTTTCCGCTGCTGTTCGGCGCATCGATCGTGATGACGATCGCATGCGT
CGTGCTCGGCGTGTTTTGA

Upstream 100 bases:

>100_bases
ATAACGGGTCGTGATTGCTGCGATCAAATCATTTCAATTGTGCTATCGCTGCGCGGACCTTACGCTCGTCTCCAACAAAA
GACAAGACAGGAGACAACCG

Downstream 100 bases:

>100_bases
TTCGATCCTTGCGGATCGCCAACCTGCCGCACGACGGTGCAGAGATGACAGCAATCCCACACGAACGGCGCGTGCGCATC
GGCGCGGGCGCCGGCTATTC

Product: CitMHS family citrate/H+ symporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MLPLLGLGTIVVLLAAILSRRMSPLVALIVVPIVASLLGGFGLQTSKFVLDGLKGLAPVVGMFVFAILYFGTITDAGTLD
PIIDRILRAVGTRPTRIVTGTTLLALLIHLDGSGAVCFLVTIPAVLPLYDRLGIDRRVLAAAVSMAAGINFLPWTGPMIR
ASASLHLPVSTLFNPLIPVQAVALVFVFGVAYWLGRREEKRLGLTRHDPAIALPKRELTADERALRRPQLFWFNIVLTLV
VLGTMVAMGEKVPPAIVFMVGLCIALTVNYPDVAMQRKRIDAHARAALMMAAILLAAGVFTGIMQGSGMLKAMAQAAVGF
VPASMAAHIPVVLGIVSMPLSMLFDPDSFYFGVLPVIAEVAGQLGVPAVKVGQAALLGQMTTGFPVSPLTPATFLVVGLC
GIELAEHQKFTFPLLFGASIVMTIACVVLGVF

Sequences:

>Translated_432_residues
MLPLLGLGTIVVLLAAILSRRMSPLVALIVVPIVASLLGGFGLQTSKFVLDGLKGLAPVVGMFVFAILYFGTITDAGTLD
PIIDRILRAVGTRPTRIVTGTTLLALLIHLDGSGAVCFLVTIPAVLPLYDRLGIDRRVLAAAVSMAAGINFLPWTGPMIR
ASASLHLPVSTLFNPLIPVQAVALVFVFGVAYWLGRREEKRLGLTRHDPAIALPKRELTADERALRRPQLFWFNIVLTLV
VLGTMVAMGEKVPPAIVFMVGLCIALTVNYPDVAMQRKRIDAHARAALMMAAILLAAGVFTGIMQGSGMLKAMAQAAVGF
VPASMAAHIPVVLGIVSMPLSMLFDPDSFYFGVLPVIAEVAGQLGVPAVKVGQAALLGQMTTGFPVSPLTPATFLVVGLC
GIELAEHQKFTFPLLFGASIVMTIACVVLGVF
>Mature_432_residues
MLPLLGLGTIVVLLAAILSRRMSPLVALIVVPIVASLLGGFGLQTSKFVLDGLKGLAPVVGMFVFAILYFGTITDAGTLD
PIIDRILRAVGTRPTRIVTGTTLLALLIHLDGSGAVCFLVTIPAVLPLYDRLGIDRRVLAAAVSMAAGINFLPWTGPMIR
ASASLHLPVSTLFNPLIPVQAVALVFVFGVAYWLGRREEKRLGLTRHDPAIALPKRELTADERALRRPQLFWFNIVLTLV
VLGTMVAMGEKVPPAIVFMVGLCIALTVNYPDVAMQRKRIDAHARAALMMAAILLAAGVFTGIMQGSGMLKAMAQAAVGF
VPASMAAHIPVVLGIVSMPLSMLFDPDSFYFGVLPVIAEVAGQLGVPAVKVGQAALLGQMTTGFPVSPLTPATFLVVGLC
GIELAEHQKFTFPLLFGASIVMTIACVVLGVF

Specific function: Transports the free citrate anion. Probably cotransports citrate and at least three or four protons. The citrate uptake is inhibited by the presence of magnesium ions [H]

COG id: COG2851

COG function: function code C; H+/citrate symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CitM (TC 2.A.11) transporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014738
- InterPro:   IPR004680 [H]

Pfam domain/function: PF03600 CitMHS [H]

EC number: NA

Molecular weight: Translated: 45663; Mature: 45663

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPLLGLGTIVVLLAAILSRRMSPLVALIVVPIVASLLGGFGLQTSKFVLDGLKGLAPVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GMFVFAILYFGTITDAGTLDPIIDRILRAVGTRPTRIVTGTTLLALLIHLDGSGAVCFLV
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCEEHHH
TIPAVLPLYDRLGIDRRVLAAAVSMAAGINFLPWTGPMIRASASLHLPVSTLFNPLIPVQ
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCEECCHHHHHCCCHHHH
AVALVFVFGVAYWLGRREEKRLGLTRHDPAIALPKRELTADERALRRPQLFWFNIVLTLV
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHCCHHHHHHCCCHHHHHHHHHHHH
VLGTMVAMGEKVPPAIVFMVGLCIALTVNYPDVAMQRKRIDAHARAALMMAAILLAAGVF
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGIMQGSGMLKAMAQAAVGFVPASMAAHIPVVLGIVSMPLSMLFDPDSFYFGVLPVIAEV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AGQLGVPAVKVGQAALLGQMTTGFPVSPLTPATFLVVGLCGIELAEHQKFTFPLLFGASI
HHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VMTIACVVLGVF
HHHHHHHHHHCC
>Mature Secondary Structure
MLPLLGLGTIVVLLAAILSRRMSPLVALIVVPIVASLLGGFGLQTSKFVLDGLKGLAPVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GMFVFAILYFGTITDAGTLDPIIDRILRAVGTRPTRIVTGTTLLALLIHLDGSGAVCFLV
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCEEHHH
TIPAVLPLYDRLGIDRRVLAAAVSMAAGINFLPWTGPMIRASASLHLPVSTLFNPLIPVQ
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCEECCHHHHHCCCHHHH
AVALVFVFGVAYWLGRREEKRLGLTRHDPAIALPKRELTADERALRRPQLFWFNIVLTLV
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHCCHHHHHHCCCHHHHHHHHHHHH
VLGTMVAMGEKVPPAIVFMVGLCIALTVNYPDVAMQRKRIDAHARAALMMAAILLAAGVF
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGIMQGSGMLKAMAQAAVGFVPASMAAHIPVVLGIVSMPLSMLFDPDSFYFGVLPVIAEV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AGQLGVPAVKVGQAALLGQMTTGFPVSPLTPATFLVVGLCGIELAEHQKFTFPLLFGASI
HHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VMTIACVVLGVF
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377; 7704256; 8892821 [H]