BacMap

Definition	Burkholderia multivorans ATCC 17616 chromosome chromosome 1, complete sequence.
Accession	NC_010084
Length	3,448,466

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The map label for this gene is braG [H]

Identifier: 161525070

GI number: 161525070

Start: 2076318

End: 2077022

Strand: Reverse

Name: braG [H]

Synonym: Bmul_1898

Alternate gene names: 161525070

Gene position: 2077022-2076318 (Counterclockwise)

Preceding gene: 161525071

Following gene: 161525069

Centisome position: 60.23

GC content: 74.33

Gene sequence:

>705_bases
ATGAGCGCGCTGCTCGACATTCGTGCGCTGCGCGCGTGGTACGGACATCAGCCGGTGCTCGACGGCATCGATCTCGCGCT
CGCGCTGGGCGAGACGCTTGCGCTGCTCGGACGCAACGGCGCCGGCCGCTCGACGCTCGCGAAGGCCGTGATGGGGCTCG
TGCGCACGAGCGGTTCGGTGCGCGTCGCGGGCGCCGAATGCGTGGGCGCACGTACCTTCGACATCGCGCGTCGCGGCGTC
GCCTACGTCGCCGAAAGCCGCGACGTGTTTCCGCTGCTCAGCGTGCGCGACAACCTGCTGCTCGGGCTGCGCGGCGTCGG
CCGGGCGGCCGCGCGCGCGGAACTCGAGCGGCTGTTCGCACGCTTTCCGCTGCTCGCCGCGCGCGCCGACGTGAAGGCCG
CGCGGCTGTCGGGCGGCGAACAGCAGGTGCTCGCGCTCGTGCGTGCGCTCGCCGGCCGCCCGCGCGTGCTGATCGTCGAC
GAACCGGCCGAGGGGCTCGCGCCGCTTGCCGTCGACGAAGTGCGCGCGTGCCTCGCCGCGCTGCAGGCGGACGGCGTCGC
GATCCTGCTGATCGAGCAGCGGTTGCAGCTGGCGCCGCGGCTGGCGCATCGCGTCGCGGTGATGGGGCGCGGAACGATCG
TCTACGACGGCGCGCTCGACGGCCTCGGCGACGACACCGTGCGCGCCTGGCTCGGCACCGGCTGA

Upstream 100 bases:

>100_bases
TCACGGTGCTCGTGCGCGGCGCGATCGTCGCGACCGGCGCACCGGAGGCGATCCGCGCGGATCCGGCCGTGCGCGCCGCC
TATCTCGGCGAGGGCACACG

Downstream 100 bases:

>100_bases
GCGCCGGCCGATTGTTCGGCTGGCCGCGCCACACAATTCGCATCGAGCCGCTTTATCGCCGCGGACCGAATCGCCACCAT
GCATGACAACGCCGGTTCGC

Product: ABC transporter-like protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSALLDIRALRAWYGHQPVLDGIDLALALGETLALLGRNGAGRSTLAKAVMGLVRTSGSVRVAGAECVGARTFDIARRGV
AYVAESRDVFPLLSVRDNLLLGLRGVGRAAARAELERLFARFPLLAARADVKAARLSGGEQQVLALVRALAGRPRVLIVD
EPAEGLAPLAVDEVRACLAALQADGVAILLIEQRLQLAPRLAHRVAVMGRGTIVYDGALDGLGDDTVRAWLGTG

Sequences:

>Translated_234_residues
MSALLDIRALRAWYGHQPVLDGIDLALALGETLALLGRNGAGRSTLAKAVMGLVRTSGSVRVAGAECVGARTFDIARRGV
AYVAESRDVFPLLSVRDNLLLGLRGVGRAAARAELERLFARFPLLAARADVKAARLSGGEQQVLALVRALAGRPRVLIVD
EPAEGLAPLAVDEVRACLAALQADGVAILLIEQRLQLAPRLAHRVAVMGRGTIVYDGALDGLGDDTVRAWLGTG
>Mature_233_residues
SALLDIRALRAWYGHQPVLDGIDLALALGETLALLGRNGAGRSTLAKAVMGLVRTSGSVRVAGAECVGARTFDIARRGVA
YVAESRDVFPLLSVRDNLLLGLRGVGRAAARAELERLFARFPLLAARADVKAARLSGGEQQVLALVRALAGRPRVLIVDE
PAEGLAPLAVDEVRACLAALQADGVAILLIEQRLQLAPRLAHRVAVMGRGTIVYDGALDGLGDDTVRAWLGTG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=207, Percent_Identity=29.951690821256, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI27436953, Length=204, Percent_Identity=28.921568627451, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI105990541, Length=204, Percent_Identity=27.4509803921569, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI21536376, Length=207, Percent_Identity=26.0869565217391, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI27477115, Length=202, Percent_Identity=30.6930693069307, Blast_Score=77, Evalue=9e-15,
Organism=Homo sapiens, GI31657092, Length=231, Percent_Identity=29.004329004329, Blast_Score=74, Evalue=8e-14,
Organism=Homo sapiens, GI150417984, Length=197, Percent_Identity=32.48730964467, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI6005701, Length=198, Percent_Identity=29.2929292929293, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI46592964, Length=219, Percent_Identity=26.9406392694064, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI46592956, Length=219, Percent_Identity=26.9406392694064, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI46592915, Length=219, Percent_Identity=26.9406392694064, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI46592978, Length=219, Percent_Identity=26.9406392694064, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI46592971, Length=219, Percent_Identity=26.9406392694064, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI46592898, Length=219, Percent_Identity=26.9406392694064, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI27262624, Length=191, Percent_Identity=28.7958115183246, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI27262626, Length=191, Percent_Identity=28.7958115183246, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI9961250, Length=211, Percent_Identity=27.4881516587678, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI4505771, Length=211, Percent_Identity=27.4881516587678, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI9961252, Length=211, Percent_Identity=27.4881516587678, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI87082268, Length=232, Percent_Identity=35.3448275862069, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1786872, Length=228, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787792, Length=230, Percent_Identity=32.6086956521739, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1789672, Length=240, Percent_Identity=30, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787029, Length=219, Percent_Identity=29.6803652968037, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788225, Length=241, Percent_Identity=31.9502074688797, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787089, Length=237, Percent_Identity=31.2236286919831, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1788761, Length=224, Percent_Identity=32.5892857142857, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788897, Length=223, Percent_Identity=30.0448430493274, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1790190, Length=208, Percent_Identity=33.1730769230769, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789593, Length=236, Percent_Identity=29.2372881355932, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789864, Length=252, Percent_Identity=28.1746031746032, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87081791, Length=221, Percent_Identity=29.4117647058824, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789991, Length=243, Percent_Identity=26.3374485596708, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI87081709, Length=219, Percent_Identity=31.0502283105023, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1786563, Length=211, Percent_Identity=31.7535545023697, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790162, Length=231, Percent_Identity=26.8398268398268, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790544, Length=246, Percent_Identity=27.6422764227642, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI48995001, Length=220, Percent_Identity=30, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1788450, Length=237, Percent_Identity=28.6919831223629, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1786319, Length=226, Percent_Identity=30.5309734513274, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787370, Length=221, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786703, Length=208, Percent_Identity=33.6538461538462, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790525, Length=232, Percent_Identity=29.3103448275862, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1787712, Length=221, Percent_Identity=28.9592760180996, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87081782, Length=227, Percent_Identity=28.1938325991189, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1789032, Length=217, Percent_Identity=31.7972350230415, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1786253, Length=247, Percent_Identity=31.1740890688259, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787500, Length=244, Percent_Identity=28.2786885245902, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788506, Length=236, Percent_Identity=32.2033898305085, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789891, Length=230, Percent_Identity=33.0434782608696, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI48994943, Length=217, Percent_Identity=29.0322580645161, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787758, Length=216, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87081834, Length=198, Percent_Identity=30.3030303030303, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787164, Length=215, Percent_Identity=32.5581395348837, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1786698, Length=161, Percent_Identity=33.5403726708075, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788472, Length=235, Percent_Identity=24.6808510638298, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789963, Length=236, Percent_Identity=28.3898305084746, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI145693107, Length=209, Percent_Identity=30.622009569378, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082267, Length=206, Percent_Identity=30.5825242718447, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786398, Length=201, Percent_Identity=29.3532338308458, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787105, Length=232, Percent_Identity=32.3275862068966, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1788165, Length=170, Percent_Identity=32.3529411764706, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788210, Length=200, Percent_Identity=28.5, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789873, Length=212, Percent_Identity=26.4150943396226, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1788644, Length=234, Percent_Identity=25.6410256410256, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790467, Length=161, Percent_Identity=33.5403726708075, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI48994883, Length=238, Percent_Identity=26.4705882352941, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786975, Length=210, Percent_Identity=27.6190476190476, Blast_Score=64, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71983523, Length=201, Percent_Identity=30.8457711442786, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71983516, Length=201, Percent_Identity=30.8457711442786, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71983510, Length=201, Percent_Identity=30.8457711442786, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17510237, Length=224, Percent_Identity=29.0178571428571, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193208177, Length=235, Percent_Identity=26.8085106382979, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115533608, Length=199, Percent_Identity=27.1356783919598, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17565586, Length=206, Percent_Identity=28.6407766990291, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17543740, Length=216, Percent_Identity=27.3148148148148, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193211015, Length=223, Percent_Identity=27.8026905829596, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193211017, Length=223, Percent_Identity=27.8026905829596, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71996809, Length=223, Percent_Identity=28.2511210762332, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17565938, Length=215, Percent_Identity=29.3023255813954, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193209708, Length=214, Percent_Identity=28.0373831775701, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193202349, Length=211, Percent_Identity=28.9099526066351, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17541710, Length=222, Percent_Identity=27.4774774774775, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI212646699, Length=212, Percent_Identity=26.4150943396226, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558664, Length=182, Percent_Identity=27.4725274725275, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17567265, Length=216, Percent_Identity=25, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25146777, Length=222, Percent_Identity=26.1261261261261, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI115535101, Length=162, Percent_Identity=28.3950617283951, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24666092, Length=227, Percent_Identity=30.3964757709251, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI221512771, Length=213, Percent_Identity=30.5164319248826, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648314, Length=218, Percent_Identity=27.0642201834862, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24643674, Length=220, Percent_Identity=28.1818181818182, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28574533, Length=215, Percent_Identity=27.4418604651163, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116007184, Length=215, Percent_Identity=26.9767441860465, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221500365, Length=215, Percent_Identity=26.9767441860465, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574259, Length=171, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281363262, Length=221, Percent_Identity=27.1493212669683, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24653245, Length=221, Percent_Identity=27.1493212669683, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28571209, Length=228, Percent_Identity=27.1929824561404, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641565, Length=219, Percent_Identity=30.5936073059361, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 24596; Mature: 24465

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALLDIRALRAWYGHQPVLDGIDLALALGETLALLGRNGAGRSTLAKAVMGLVRTSGSV
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCE
RVAGAECVGARTFDIARRGVAYVAESRDVFPLLSVRDNLLLGLRGVGRAAARAELERLFA
EEECHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHCCCCCHHHHHHHHHHHHH
RFPLLAARADVKAARLSGGEQQVLALVRALAGRPRVLIVDEPAEGLAPLAVDEVRACLAA
HCCHHHHHCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LQADGVAILLIEQRLQLAPRLAHRVAVMGRGTIVYDGALDGLGDDTVRAWLGTG
HHCCCEEEEEEHHHHHHHHHHHHHHHHEECCEEEECCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
SALLDIRALRAWYGHQPVLDGIDLALALGETLALLGRNGAGRSTLAKAVMGLVRTSGSV
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCE
RVAGAECVGARTFDIARRGVAYVAESRDVFPLLSVRDNLLLGLRGVGRAAARAELERLFA
EEECHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHCCCCCHHHHHHHHHHHHH
RFPLLAARADVKAARLSGGEQQVLALVRALAGRPRVLIVDEPAEGLAPLAVDEVRACLAA
HCCHHHHHCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LQADGVAILLIEQRLQLAPRLAHRVAVMGRGTIVYDGALDGLGDDTVRAWLGTG
HHCCCEEEEEEHHHHHHHHHHHHHHHHEECCEEEECCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]