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The map label for this gene is rimO [H]

Identifier: 161524661

GI number: 161524661

Start: 1639101

End: 1640507

Strand: Direct

Name: rimO [H]

Synonym: Bmul_1488

Alternate gene names: 161524661

Gene position: 1639101-1640507 (Clockwise)

Preceding gene: 161524659

Following gene: 161524662

Centisome position: 47.53

GC content: 64.32

Gene sequence:

>1407_bases
ATGGCTGTAAACTCACGGTTTTGCTGCCACGCCCCCACACTCCAGATGTCTCAATCCCCCAAAGTCGGCTTCGTTTCCCT
CGGCTGCCCGAAAGCGCTCGTCGACTCCGAACAGATCATCACCCAGCTGCGCGCCGAAGGCTATGAAATCTCCGGCACCT
ACGACGGCGCCGACCTCGTCGTCGTCAACACGTGCGGCTTCATCGACGAAGCGGTGCAGGAAAGCCTCGACGCGATCGGC
GAAGCGCTGACCGAGAACGGCAAGGTGATCGTCACCGGCTGTCTCGGCGCGAAGAAGAGCGCGAGCGGCTCGGGCCTGAT
CGAGGAAGTGCACCCGAAGGTGCTCGCCGTCACGGGCCCGCATGCGGTCGGCGAAGTGATGCAGGCCGTGCACTCGCACC
TGCCGAAGCCGCACGATCCGTTCGTCGATCTCGTGCCGCCGGCCGGCATCAAGCTCACGCCGCGTCACTATGCGTACCTG
AAGATTTCCGAAGGCTGCAACCACCGCTGCACGTTCTGCATCATCCCGTCGATGCGCGGCGATCTGGTGTCGCGTCCCGT
CGCCGAAGTGATGCTCGAGGCGGAAAACCTGTTCAAGTCGGGCGTGAAGGAACTGCTCGTGATCTCGCAGGACACGAGCG
CATACGGCGTCGACGTCAAATACCGCACGGGCTTCTGGAACGGCAAGCCGATCAAGACGCGCATGACCGATCTCGTCGCC
GCGCTCGGCGAACTCGCCGCGCAGTACGGCGCGTGGGTGCGTCTGCATTACGTGTATCCGTATCCGAGCGTCGACGAAGT
GATTCCGCTGATGGCCGAGGGTCCGTTCAAGGGCCACGTGCTGCCGTATCTCGACGTGCCGTTCCAGCACGCGCATCCGG
ACGTGCTGAAGCGGATGAAGCGTCCGGCGAACGCCGAGAAGGTGCTCGAGCGCGTGCAGAAGTGGCGCGAGATCTGCCCC
GATCTGACGATCCGCAGCACGTTCATCGCGGGCTTTCCGGGCGAGACGGAAGCACAGTTCGAAACGCTGCTCGACTTCAT
CCGCGAGGCGGAACTCGATCGCGTGGGCTGCTTCGCGTATTCGCCGGTCGAAGGCGCGAGTGCGAATGAACTCGACGGCG
CGCTGCCGGACGATGTCCGCGAGGAACGTCGCGCGCGCTTCATGGAAGTCGCCGAGGAAGTGTCGGCCCGGCGCATCGCG
CGCAAGGTCGGCAAGACGCTGAAGGTGCTGATCGACGAAGTCAGCGACGAAGGCGGTATCGGACGCACGGCGGCCGATGC
GCCGGAGATCGACGGCGTCGTCTATGTCGAGCCGGCGACCAAGGCATCGAAGCGCTACAAGGTCGGCGATTTCGTGTCGG
TGAAGATCACCGGCGCGGACGGCCACGACCTGTGGGGCGAGGTGTAA

Upstream 100 bases:

>100_bases
GCAGGACCGGCGCCGGGCCGCTCCGGCGCGGCAGCATTCGTTGCCGAAAGGGCCGCATCGCTGCACGCATTCGCGGCCGG
TTTTGGCTCGGCGAACCTAA

Downstream 100 bases:

>100_bases
GCGATGAGCGCCGCGTTTCCGGAGATCCTCGCGCTCGGCGAGGCGATGATCGAGTTCAACCAGGCGCAGCCCGGCCGTGC
CGAGTTCCTGCAGGGCTTCG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MAVNSRFCCHAPTLQMSQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIG
EALTENGKVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHAVGEVMQAVHSHLPKPHDPFVDLVPPAGIKLTPRHYAYL
KISEGCNHRCTFCIIPSMRGDLVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVA
ALGELAAQYGAWVRLHYVYPYPSVDEVIPLMAEGPFKGHVLPYLDVPFQHAHPDVLKRMKRPANAEKVLERVQKWREICP
DLTIRSTFIAGFPGETEAQFETLLDFIREAELDRVGCFAYSPVEGASANELDGALPDDVREERRARFMEVAEEVSARRIA
RKVGKTLKVLIDEVSDEGGIGRTAADAPEIDGVVYVEPATKASKRYKVGDFVSVKITGADGHDLWGEV

Sequences:

>Translated_468_residues
MAVNSRFCCHAPTLQMSQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIG
EALTENGKVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHAVGEVMQAVHSHLPKPHDPFVDLVPPAGIKLTPRHYAYL
KISEGCNHRCTFCIIPSMRGDLVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVA
ALGELAAQYGAWVRLHYVYPYPSVDEVIPLMAEGPFKGHVLPYLDVPFQHAHPDVLKRMKRPANAEKVLERVQKWREICP
DLTIRSTFIAGFPGETEAQFETLLDFIREAELDRVGCFAYSPVEGASANELDGALPDDVREERRARFMEVAEEVSARRIA
RKVGKTLKVLIDEVSDEGGIGRTAADAPEIDGVVYVEPATKASKRYKVGDFVSVKITGADGHDLWGEV
>Mature_467_residues
AVNSRFCCHAPTLQMSQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGE
ALTENGKVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGPHAVGEVMQAVHSHLPKPHDPFVDLVPPAGIKLTPRHYAYLK
ISEGCNHRCTFCIIPSMRGDLVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVAA
LGELAAQYGAWVRLHYVYPYPSVDEVIPLMAEGPFKGHVLPYLDVPFQHAHPDVLKRMKRPANAEKVLERVQKWREICPD
LTIRSTFIAGFPGETEAQFETLLDFIREAELDRVGCFAYSPVEGASANELDGALPDDVREERRARFMEVAEEVSARRIAR
KVGKTLKVLIDEVSDEGGIGRTAADAPEIDGVVYVEPATKASKRYKVGDFVSVKITGADGHDLWGEV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=494, Percent_Identity=25.3036437246964, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI93277076, Length=414, Percent_Identity=27.0531400966184, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI28872782, Length=488, Percent_Identity=25.4098360655738, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI1787057, Length=453, Percent_Identity=63.7969094922737, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1786882, Length=467, Percent_Identity=26.7665952890792, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=483, Percent_Identity=24.6376811594203, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71996771, Length=302, Percent_Identity=26.4900662251656, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356207, Length=485, Percent_Identity=28.4536082474227, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19922432, Length=469, Percent_Identity=27.2921108742004, Blast_Score=119, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 51089; Mature: 50958

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVNSRFCCHAPTLQMSQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLV
CCCCCCCEECCCCCCCCCCCCCEEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEE
VVNTCGFIDEAVQESLDAIGEALTENGKVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGP
EEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHCCCEEEEECC
HAVGEVMQAVHSHLPKPHDPFVDLVPPAGIKLTPRHYAYLKISEGCNHRCTFCIIPSMRG
HHHHHHHHHHHHHCCCCCCCCEECCCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCC
DLVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVA
HHHHCCHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHH
ALGELAAQYGAWVRLHYVYPYPSVDEVIPLMAEGPFKGHVLPYLDVPFQHAHPDVLKRMK
HHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHH
RPANAEKVLERVQKWREICPDLTIRSTFIAGFPGETEAQFETLLDFIREAELDRVGCFAY
CCCCHHHHHHHHHHHHHHCCCCEEEHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
SPVEGASANELDGALPDDVREERRARFMEVAEEVSARRIARKVGKTLKVLIDEVSDEGGI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GRTAADAPEIDGVVYVEPATKASKRYKVGDFVSVKITGADGHDLWGEV
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
AVNSRFCCHAPTLQMSQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLV
CCCCCCEECCCCCCCCCCCCCEEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEE
VVNTCGFIDEAVQESLDAIGEALTENGKVIVTGCLGAKKSASGSGLIEEVHPKVLAVTGP
EEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHCCCEEEEECC
HAVGEVMQAVHSHLPKPHDPFVDLVPPAGIKLTPRHYAYLKISEGCNHRCTFCIIPSMRG
HHHHHHHHHHHHHCCCCCCCCEECCCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCC
DLVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGKPIKTRMTDLVA
HHHHCCHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHH
ALGELAAQYGAWVRLHYVYPYPSVDEVIPLMAEGPFKGHVLPYLDVPFQHAHPDVLKRMK
HHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHH
RPANAEKVLERVQKWREICPDLTIRSTFIAGFPGETEAQFETLLDFIREAELDRVGCFAY
CCCCHHHHHHHHHHHHHHCCCCEEEHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
SPVEGASANELDGALPDDVREERRARFMEVAEEVSARRIARKVGKTLKVLIDEVSDEGGI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GRTAADAPEIDGVVYVEPATKASKRYKVGDFVSVKITGADGHDLWGEV
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA