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The map label for this gene is clpB [H]

Identifier: 161524599

GI number: 161524599

Start: 1568687

End: 1571284

Strand: Direct

Name: clpB [H]

Synonym: Bmul_1426

Alternate gene names: 161524599

Gene position: 1568687-1571284 (Clockwise)

Preceding gene: 161524598

Following gene: 161524602

Centisome position: 45.49

GC content: 66.17

Gene sequence:

>2598_bases
ATGAGAATCGACAAGCTCACCACCAAGTTCCAGGAAGCGCTTGCGGATGCGCAAAGCCTCGCGGCCGGCCGCGACAATCA
ATACATCGAACCGGTTCACGTGCTGGCGGCGCTGCTCGCGCAGCAGGACGGTTCCGCGCGCTCGCTGATGTCGCGGGCGG
GCGTCCACGTGCAGGCGCTGCAGGGCGCGCTGAACGACGCGATCGCACGCCTGCCGCAGGTCACGGGCACGGGCGGCGAC
ATCCAGGTCGGCCGCGAGCTGGCCGGCCTGCTGAACCAGGCCGACAAGGAAGCGCAGAAGCTCAACGACACGTTCATCGC
GAGCGAGATGTTCCTGCTCGCGGTCGCGGACGACAAGGGCGAAGCAGGCAAGCTCGCGCGTCAGCACGGCCTCACGCGCA
AGGCGCTCGAAGCCGCGATCGCCGCGGTGCGCGGCGGCTCGCAGGTGCATAGCCAGGACGCGGAAAGCCAGCGCGAAGCG
CTGAAGAAATACACGGTCGACCTGACCGAGCGCGCACGCGCCGGCAAGCTCGATCCCGTGATCGGCCGCGACGACGAAAT
CCGCCGCTCGATCCAGATTCTGCAACGCCGCACCAAGAACAACCCCGTGCTGATCGGCGAGCCGGGCGTCGGCAAGACGG
CCATCGTCGAAGGGCTCGCGCAGCGGATCGTCAACGGCGAGGTGCCGGAGACGCTGAAGGGCAAGCGCGTGCTGTCGCTC
GACATGGCTGCGCTGCTCGCCGGCGCGAAGTATCGCGGCGAGTTCGAGGAACGCCTGAAGGCCGTGCTCAACGACATCGC
GAAGGACGAAGGCCAGACGATCGTGTTCATCGACGAGATCCACACGATGGTCGGCGCCGGCAAGGCCGAAGGCGCGATGG
ATGCGGGCAACATGCTGAAGCCGGCGCTGTCGCGCGGCGAGCTGCACTGCATCGGCGCGACCACGCTCGACGAATACCGG
AAGTACATCGAGAAGGATGCCGCGCTCGAGCGCCGCTTCCAGAAGGTGCTCGTCGACGAGCCGAGCGTCGAGGCGACGAT
CGCGATCCTGCGCGGGCTGCAGGAGAAGTACGAACTGCACCATGGCGTCGACATCACGGACCCGGCGATCGTCGCCGCGG
CCGAGCTGTCGCATCGCTACATCACGGACCGCTTCCTGCCGGATAAGGCGATCGACCTGATCGACGAGGCCGCTTCGAAG
ATCAAGATGGAAATCGATTCGAAGCCCGAAGAGATGGACAAGCTCGACCGCCGGCTGATCCAGCTGAAGATCGAGCGCGA
GGCCGTGAAGAAGGAGCAGGACGAAGCGTCGCAGAAGCGGCTGCAGCTGATCGAGGAAGAGATCGAGCGGCTCGGGCGCG
AGTATGCGGACCTCGAGGAAATCTGGACCGCCGAGAAGGCCGCGGTGCAGGGCAGCGCGCAGCTGAAGGAAGAGATCGAG
AAGGTGCGCGCGGACATCGCGCGGCTGCAGCGTGAAGGCAAGCTCGAGAAGGTCGCCGAACTGCAGTACGGGAAGCTGCC
GCAGCTCGAGGCGCAGCTGAAGCAGGTCACGCAGGCCGAAGAGCAGGAGCAGCACAATCCGACGCGGCCGCGTCTGCTGC
GCACGCACGTCGGCGCCGAGGAGATCGCGGAAGTCGTGTCGCGCGCGACCGGGATTCCGGTGTCGCGAATGATGCAGGGC
GAACGCGAAAAGCTGCTGCACATCGAGGAGAAGCTGCACGAGCGCGTGGTCGGCCAGGACGAGGCGATCAACGCGGTCGC
CGATGCGATCCGCCGCTCGCGCGCGGGCCTCGCCGATCCGAACCGTCCGTACGGCTCGTTCCTGTTCCTCGGCCCGACGG
GCGTCGGCAAGACCGAGCTGTGCAAGGCGCTCGCGTCGTTCCTGTTCGATTCGGAAGAGCATCTGATCCGCATCGACATG
AGCGAGTTCATGGAGAAGCACAGCGTGGCGCGGCTGATCGGCGCGCCGCCGGGCTACGTCGGCTACGAGGAGGGCGGCTA
CCTGACGGAAGCCGTGCGCCGCAAGCCGTACAGCGTGATCCTGCTCGACGAAATCGAGAAGGCGCATCCGGACGTGTTCA
ACGTGCTGCTGCAGGTGCTCGACGACGGTCGCATGACCGACGGGCAGGGCCGTACGGTCGACTTCAAGAACACGGTGATC
GTCATGACGTCGAACCTCGGCTCGCAGGTGATCCAGTCGATGACGGGCTCGCCGCAGGACGAGATCAAGGATGCGGTGTG
GCTCGAGGTGAAGCAGCATTTCCGCCCCGAGTTCCTGAACCGGATCGACGACGTCGTCGTGTTCCACGGCCTCGATCGCC
ACAACATCGAGTCGATCGCGAAGATCCAGCTCGCGCGCCTGCACGACCGGCTCGCGAAGCTCGACATGGCGCTCGACGTG
TCGCCGGCGGCGCTCGAGCAGATCGCGAAGGTCGGCTACGATCCGCTGTTCGGTGCACGGCCGCTGAAGCGCGCGATCCA
GCAGGAGATCGAGAACCCGGTCGCGAAGCTGATCCTCGCCGGCCGCTTCGGCCCGAAGGACGTGATTCCGGTCGACGTCA
ACGACGGCAAGTTCGTGTTCGACCGCGTCGTGCACTGA

Upstream 100 bases:

>100_bases
CCGCCGACACTTTTTTGCGCTGTATCGACCCGAGTTCCGCTTGAAAGCCGCATAACCATCCTCACTTTGGTGTTAATCGA
AAATTGGAGGTCTCAACTAA

Downstream 100 bases:

>100_bases
TCGCCGTCATCGGCAGCGTCGCCCGGTTCGCGCCGGGCGCGCAAAAAACGAACACCCCGCCTCGGCGGGGTGTTTGCTTA
TGGGGCCGCGTACGACGGCG

Product: ATP-dependent chaperone ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 865; Mature: 865

Protein sequence:

>865_residues
MRIDKLTTKFQEALADAQSLAAGRDNQYIEPVHVLAALLAQQDGSARSLMSRAGVHVQALQGALNDAIARLPQVTGTGGD
IQVGRELAGLLNQADKEAQKLNDTFIASEMFLLAVADDKGEAGKLARQHGLTRKALEAAIAAVRGGSQVHSQDAESQREA
LKKYTVDLTERARAGKLDPVIGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKGKRVLSL
DMAALLAGAKYRGEFEERLKAVLNDIAKDEGQTIVFIDEIHTMVGAGKAEGAMDAGNMLKPALSRGELHCIGATTLDEYR
KYIEKDAALERRFQKVLVDEPSVEATIAILRGLQEKYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASK
IKMEIDSKPEEMDKLDRRLIQLKIEREAVKKEQDEASQKRLQLIEEEIERLGREYADLEEIWTAEKAAVQGSAQLKEEIE
KVRADIARLQREGKLEKVAELQYGKLPQLEAQLKQVTQAEEQEQHNPTRPRLLRTHVGAEEIAEVVSRATGIPVSRMMQG
EREKLLHIEEKLHERVVGQDEAINAVADAIRRSRAGLADPNRPYGSFLFLGPTGVGKTELCKALASFLFDSEEHLIRIDM
SEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI
VMTSNLGSQVIQSMTGSPQDEIKDAVWLEVKQHFRPEFLNRIDDVVVFHGLDRHNIESIAKIQLARLHDRLAKLDMALDV
SPAALEQIAKVGYDPLFGARPLKRAIQQEIENPVAKLILAGRFGPKDVIPVDVNDGKFVFDRVVH

Sequences:

>Translated_865_residues
MRIDKLTTKFQEALADAQSLAAGRDNQYIEPVHVLAALLAQQDGSARSLMSRAGVHVQALQGALNDAIARLPQVTGTGGD
IQVGRELAGLLNQADKEAQKLNDTFIASEMFLLAVADDKGEAGKLARQHGLTRKALEAAIAAVRGGSQVHSQDAESQREA
LKKYTVDLTERARAGKLDPVIGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKGKRVLSL
DMAALLAGAKYRGEFEERLKAVLNDIAKDEGQTIVFIDEIHTMVGAGKAEGAMDAGNMLKPALSRGELHCIGATTLDEYR
KYIEKDAALERRFQKVLVDEPSVEATIAILRGLQEKYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASK
IKMEIDSKPEEMDKLDRRLIQLKIEREAVKKEQDEASQKRLQLIEEEIERLGREYADLEEIWTAEKAAVQGSAQLKEEIE
KVRADIARLQREGKLEKVAELQYGKLPQLEAQLKQVTQAEEQEQHNPTRPRLLRTHVGAEEIAEVVSRATGIPVSRMMQG
EREKLLHIEEKLHERVVGQDEAINAVADAIRRSRAGLADPNRPYGSFLFLGPTGVGKTELCKALASFLFDSEEHLIRIDM
SEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI
VMTSNLGSQVIQSMTGSPQDEIKDAVWLEVKQHFRPEFLNRIDDVVVFHGLDRHNIESIAKIQLARLHDRLAKLDMALDV
SPAALEQIAKVGYDPLFGARPLKRAIQQEIENPVAKLILAGRFGPKDVIPVDVNDGKFVFDRVVH
>Mature_865_residues
MRIDKLTTKFQEALADAQSLAAGRDNQYIEPVHVLAALLAQQDGSARSLMSRAGVHVQALQGALNDAIARLPQVTGTGGD
IQVGRELAGLLNQADKEAQKLNDTFIASEMFLLAVADDKGEAGKLARQHGLTRKALEAAIAAVRGGSQVHSQDAESQREA
LKKYTVDLTERARAGKLDPVIGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKGKRVLSL
DMAALLAGAKYRGEFEERLKAVLNDIAKDEGQTIVFIDEIHTMVGAGKAEGAMDAGNMLKPALSRGELHCIGATTLDEYR
KYIEKDAALERRFQKVLVDEPSVEATIAILRGLQEKYELHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASK
IKMEIDSKPEEMDKLDRRLIQLKIEREAVKKEQDEASQKRLQLIEEEIERLGREYADLEEIWTAEKAAVQGSAQLKEEIE
KVRADIARLQREGKLEKVAELQYGKLPQLEAQLKQVTQAEEQEQHNPTRPRLLRTHVGAEEIAEVVSRATGIPVSRMMQG
EREKLLHIEEKLHERVVGQDEAINAVADAIRRSRAGLADPNRPYGSFLFLGPTGVGKTELCKALASFLFDSEEHLIRIDM
SEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI
VMTSNLGSQVIQSMTGSPQDEIKDAVWLEVKQHFRPEFLNRIDDVVVFHGLDRHNIESIAKIQLARLHDRLAKLDMALDV
SPAALEQIAKVGYDPLFGARPLKRAIQQEIENPVAKLILAGRFGPKDVIPVDVNDGKFVFDRVVH

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=298, Percent_Identity=35.9060402684564, Blast_Score=192, Evalue=1e-48,
Organism=Escherichia coli, GI1788943, Length=860, Percent_Identity=66.6279069767442, Blast_Score=1167, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=398, Percent_Identity=41.4572864321608, Blast_Score=283, Evalue=5e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=712, Percent_Identity=52.8089887640449, Blast_Score=756, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=865, Percent_Identity=43.0057803468208, Blast_Score=692, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95949; Mature: 95949

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDKLTTKFQEALADAQSLAAGRDNQYIEPVHVLAALLAQQDGSARSLMSRAGVHVQAL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHH
QGALNDAIARLPQVTGTGGDIQVGRELAGLLNQADKEAQKLNDTFIASEMFLLAVADDKG
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCC
EAGKLARQHGLTRKALEAAIAAVRGGSQVHSQDAESQREALKKYTVDLTERARAGKLDPV
CHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKGKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHCCCEEHHH
DMAALLAGAKYRGEFEERLKAVLNDIAKDEGQTIVFIDEIHTMVGAGKAEGAMDAGNMLK
HHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHH
PALSRGELHCIGATTLDEYRKYIEKDAALERRFQKVLVDEPSVEATIAILRGLQEKYELH
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASKIKMEIDSKPEEMDKLDRRLI
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEHHCCCCHHHHHHHHHHHH
QLKIEREAVKKEQDEASQKRLQLIEEEIERLGREYADLEEIWTAEKAAVQGSAQLKEEIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KVRADIARLQREGKLEKVAELQYGKLPQLEAQLKQVTQAEEQEQHNPTRPRLLRTHVGAE
HHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHH
EIAEVVSRATGIPVSRMMQGEREKLLHIEEKLHERVVGQDEAINAVADAIRRSRAGLADP
HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
NRPYGSFLFLGPTGVGKTELCKALASFLFDSEEHLIRIDMSEFMEKHSVARLIGAPPGYV
CCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC
GYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI
CCCCCCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEE
VMTSNLGSQVIQSMTGSPQDEIKDAVWLEVKQHFRPEFLNRIDDVVVFHGLDRHNIESIA
EEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHH
KIQLARLHDRLAKLDMALDVSPAALEQIAKVGYDPLFGARPLKRAIQQEIENPVAKLILA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GRFGPKDVIPVDVNDGKFVFDRVVH
CCCCCCCEEEEECCCCCHHHHHHCC
>Mature Secondary Structure
MRIDKLTTKFQEALADAQSLAAGRDNQYIEPVHVLAALLAQQDGSARSLMSRAGVHVQAL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHH
QGALNDAIARLPQVTGTGGDIQVGRELAGLLNQADKEAQKLNDTFIASEMFLLAVADDKG
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCC
EAGKLARQHGLTRKALEAAIAAVRGGSQVHSQDAESQREALKKYTVDLTERARAGKLDPV
CHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IGRDDEIRRSIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPETLKGKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHCCCEEHHH
DMAALLAGAKYRGEFEERLKAVLNDIAKDEGQTIVFIDEIHTMVGAGKAEGAMDAGNMLK
HHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHH
PALSRGELHCIGATTLDEYRKYIEKDAALERRFQKVLVDEPSVEATIAILRGLQEKYELH
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASKIKMEIDSKPEEMDKLDRRLI
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEHHCCCCHHHHHHHHHHHH
QLKIEREAVKKEQDEASQKRLQLIEEEIERLGREYADLEEIWTAEKAAVQGSAQLKEEIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KVRADIARLQREGKLEKVAELQYGKLPQLEAQLKQVTQAEEQEQHNPTRPRLLRTHVGAE
HHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHH
EIAEVVSRATGIPVSRMMQGEREKLLHIEEKLHERVVGQDEAINAVADAIRRSRAGLADP
HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
NRPYGSFLFLGPTGVGKTELCKALASFLFDSEEHLIRIDMSEFMEKHSVARLIGAPPGYV
CCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC
GYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVI
CCCCCCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEE
VMTSNLGSQVIQSMTGSPQDEIKDAVWLEVKQHFRPEFLNRIDDVVVFHGLDRHNIESIA
EEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHH
KIQLARLHDRLAKLDMALDVSPAALEQIAKVGYDPLFGARPLKRAIQQEIENPVAKLILA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GRFGPKDVIPVDVNDGKFVFDRVVH
CCCCCCCEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]