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The map label for this gene is pleD [H]

Identifier: 161524544

GI number: 161524544

Start: 1513958

End: 1515358

Strand: Direct

Name: pleD [H]

Synonym: Bmul_1371

Alternate gene names: 161524544

Gene position: 1513958-1515358 (Clockwise)

Preceding gene: 161524543

Following gene: 161524546

Centisome position: 43.9

GC content: 67.52

Gene sequence:

>1401_bases
TTGAGTACGACCTTTGCAGCCGGGGAACCGCCCCGCGTCGTGAGCGCGGCTGCGCGGATTTACGCGAAATGCCTGCTGGT
CGGATTCGCGCTGCTGCCCGCCTATCTCATCGCCTACCTGTGGTTCTGCGCCGATCCGCGCGCGACCTTCGAGAACCACG
CATTCCATGAAATCGCGATTGCGGCCGCGACCGTCGAGGGCGCGTTCGTCACGTACGTCACCTGGGTCTGCTATCGATCG
TCGGGCGAACCGCTGTTGCGCTGGCTCACGCTCGGCTTTCTCGGCTTCGCGATGATCTACGCGCTGCACGGCGTGTTTAC
CGGCGCCGCGCATCACAACATCTGGCTGTTCCTGCTCTACGGGCCCGCGTCGCGGCTCGTGATGTCGATCCTGCTGCTGA
CCGGCCTGCTGTCGTATTCGCGCGCGCCCGATCGCGCCGACAGGCGCACGAGCGCGCGCACGTGGCTGCCGTGGATCGTC
GCGTTCGCGCTCGTCGACGTCGCGGTCGCGTATCTCGCGTATTCGCCGATCGCGGGCGCACTCGGCACGCGGCTCGCGAT
GGAGGGCGGTGCGCTCGTATTCTCGCTGCTGAACGTCGCCGTGCTGCTCGCGCGACGCATCCGTTCGCCGCTGATGGTGA
TCTACGGCGTGTCGATCACGGCGTTCGCGCTGTCGTCGCTCGCGTTCCTGCTCGGCAAGCCGTGGAACCACATGTGGTGG
CTCGCGCACGCGATCTTCGCGGGCGGCTTCTTTTTGCTCAGCTACGGCGTCGTGCAGGCGCTGCAGACGACGCGCTCGTT
CTCGGCGATCTACAGCCAGCAGGATCTGATGAGCCGCCTCGCGGAATCGATGGCGCGCACCGAGAGCGCGCTGCTCGAAC
TGCGACGCACGAACCAGAAGCTCGAATACATGGCCGCGACCGATCCGATGACGGGCGCGTCGAACCGCCGGCAATTCATC
GCGCAGGTCGAACGCGAGATCGGCCGCGCCGAGCGCGACGGCACACCGTTCTGCCTGCTCGCGCTCGACCTCGACAATTT
CAAGCACATCAACGACGCGTACGGCCATCAGATCGGCGACGAGGTGCCGCGCGGCGTCGTGCGCCAGTGCCTCGACGCGA
TCCGGCCGAGCGGCGCGCTCGCGCGCGTCGGCGGCGAGGAGTTCATGGCCTTGCTGCCCGAGATGCCGCTCGAGGGGGCA
CGGATGACGGCCGAGCGCGTGCGTTCGTCGATCGCCACTGCGCCGTTCGGCCTCGACTTCAAGCGCGTGCAGGTCACGGT
CAGTGTCGGTGTGGCGCAGTACGGCGTCGACGGCAGCACGGTCGACGCGCTGCTGCGCGTCGTCGACGAACGGCTGTATC
AGGCGAAGCGGGAAGGACGCAATCGCGTCGTCGCGCGGTGA

Upstream 100 bases:

>100_bases
TTTGCCTTTCGGAACAGAGGGGCCGACGCGCGCGCCATGGCCGGGTCGCGCGCTTGCGGCTGCGACACGCGAATCCGCAG
CAAATCGATCGGGGAACGAA

Downstream 100 bases:

>100_bases
CGCTTTGCGCGGCAATGCATTTGCCCGGACCGGTGTGAGCGCGTGCCTTCCCGTACGTCGCCCCGGACGGCGAAGCCGTG
CCGGGGCGACATCGCGCGAT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSTTFAAGEPPRVVSAAARIYAKCLLVGFALLPAYLIAYLWFCADPRATFENHAFHEIAIAAATVEGAFVTYVTWVCYRS
SGEPLLRWLTLGFLGFAMIYALHGVFTGAAHHNIWLFLLYGPASRLVMSILLLTGLLSYSRAPDRADRRTSARTWLPWIV
AFALVDVAVAYLAYSPIAGALGTRLAMEGGALVFSLLNVAVLLARRIRSPLMVIYGVSITAFALSSLAFLLGKPWNHMWW
LAHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQQDLMSRLAESMARTESALLELRRTNQKLEYMAATDPMTGASNRRQFI
AQVEREIGRAERDGTPFCLLALDLDNFKHINDAYGHQIGDEVPRGVVRQCLDAIRPSGALARVGGEEFMALLPEMPLEGA
RMTAERVRSSIATAPFGLDFKRVQVTVSVGVAQYGVDGSTVDALLRVVDERLYQAKREGRNRVVAR

Sequences:

>Translated_466_residues
MSTTFAAGEPPRVVSAAARIYAKCLLVGFALLPAYLIAYLWFCADPRATFENHAFHEIAIAAATVEGAFVTYVTWVCYRS
SGEPLLRWLTLGFLGFAMIYALHGVFTGAAHHNIWLFLLYGPASRLVMSILLLTGLLSYSRAPDRADRRTSARTWLPWIV
AFALVDVAVAYLAYSPIAGALGTRLAMEGGALVFSLLNVAVLLARRIRSPLMVIYGVSITAFALSSLAFLLGKPWNHMWW
LAHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQQDLMSRLAESMARTESALLELRRTNQKLEYMAATDPMTGASNRRQFI
AQVEREIGRAERDGTPFCLLALDLDNFKHINDAYGHQIGDEVPRGVVRQCLDAIRPSGALARVGGEEFMALLPEMPLEGA
RMTAERVRSSIATAPFGLDFKRVQVTVSVGVAQYGVDGSTVDALLRVVDERLYQAKREGRNRVVAR
>Mature_465_residues
STTFAAGEPPRVVSAAARIYAKCLLVGFALLPAYLIAYLWFCADPRATFENHAFHEIAIAAATVEGAFVTYVTWVCYRSS
GEPLLRWLTLGFLGFAMIYALHGVFTGAAHHNIWLFLLYGPASRLVMSILLLTGLLSYSRAPDRADRRTSARTWLPWIVA
FALVDVAVAYLAYSPIAGALGTRLAMEGGALVFSLLNVAVLLARRIRSPLMVIYGVSITAFALSSLAFLLGKPWNHMWWL
AHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQQDLMSRLAESMARTESALLELRRTNQKLEYMAATDPMTGASNRRQFIA
QVEREIGRAERDGTPFCLLALDLDNFKHINDAYGHQIGDEVPRGVVRQCLDAIRPSGALARVGGEEFMALLPEMPLEGAR
MTAERVRSSIATAPFGLDFKRVQVTVSVGVAQYGVDGSTVDALLRVVDERLYQAKREGRNRVVAR

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=168, Percent_Identity=36.3095238095238, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=42.0454545454545, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI145693134, Length=212, Percent_Identity=32.0754716981132, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=34.4632768361582, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=34.3023255813954, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=32.9268292682927, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1787802, Length=157, Percent_Identity=35.031847133758, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=31.5789473684211, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=32.9341317365269, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788956, Length=256, Percent_Identity=25.78125, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081974, Length=168, Percent_Identity=27.9761904761905, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787056, Length=158, Percent_Identity=31.0126582278481, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=28.6624203821656, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51285; Mature: 51154

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTFAAGEPPRVVSAAARIYAKCLLVGFALLPAYLIAYLWFCADPRATFENHAFHEIAI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHH
AAATVEGAFVTYVTWVCYRSSGEPLLRWLTLGFLGFAMIYALHGVFTGAAHHNIWLFLLY
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
GPASRLVMSILLLTGLLSYSRAPDRADRRTSARTWLPWIVAFALVDVAVAYLAYSPIAGA
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTRLAMEGGALVFSLLNVAVLLARRIRSPLMVIYGVSITAFALSSLAFLLGKPWNHMWW
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHHH
LAHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQQDLMSRLAESMARTESALLELRRTNQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
LEYMAATDPMTGASNRRQFIAQVEREIGRAERDGTPFCLLALDLDNFKHINDAYGHQIGD
HHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
EVPRGVVRQCLDAIRPSGALARVGGEEFMALLPEMPLEGARMTAERVRSSIATAPFGLDF
HHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCC
KRVQVTVSVGVAQYGVDGSTVDALLRVVDERLYQAKREGRNRVVAR
EEEEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
STTFAAGEPPRVVSAAARIYAKCLLVGFALLPAYLIAYLWFCADPRATFENHAFHEIAI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHH
AAATVEGAFVTYVTWVCYRSSGEPLLRWLTLGFLGFAMIYALHGVFTGAAHHNIWLFLLY
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
GPASRLVMSILLLTGLLSYSRAPDRADRRTSARTWLPWIVAFALVDVAVAYLAYSPIAGA
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTRLAMEGGALVFSLLNVAVLLARRIRSPLMVIYGVSITAFALSSLAFLLGKPWNHMWW
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHHH
LAHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQQDLMSRLAESMARTESALLELRRTNQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
LEYMAATDPMTGASNRRQFIAQVEREIGRAERDGTPFCLLALDLDNFKHINDAYGHQIGD
HHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
EVPRGVVRQCLDAIRPSGALARVGGEEFMALLPEMPLEGARMTAERVRSSIATAPFGLDF
HHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCC
KRVQVTVSVGVAQYGVDGSTVDALLRVVDERLYQAKREGRNRVVAR
EEEEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA