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The map label for this gene is alkK [H]

Identifier: 161524540

GI number: 161524540

Start: 1507823

End: 1509466

Strand: Direct

Name: alkK [H]

Synonym: Bmul_1367

Alternate gene names: 161524540

Gene position: 1507823-1509466 (Clockwise)

Preceding gene: 161524534

Following gene: 161524541

Centisome position: 43.72

GC content: 68.25

Gene sequence:

>1644_bases
ATGGGCAAGCCGTTGCTGGGCCAGATGATGGACATGCCGCTGCTGGTGTCGTCGCTGATCGCGCATGCCGCGCGTCACGC
GGGCGACACCGAGATCGTGTCGAAGCGGATCGAAGGCGACCTGCATCGCTATACATATCGCGACTGCGAACGGCGCGCGA
AGCGGCTCGCGCAGGCGCTCGCGCGGCTCGGCGTCGAGGCGGGCGACCGCGTCGGCACGCTCGCGTGGAACGGCTACCGG
CATCTCGAGGCGTACTACGCGATCGGCGGCATGGGCGCCGTCTGCCACACGATCAACCCGCGGCTTTTTCCCGAACAGAT
CGCGTACATCGTCAATCACGCGGAAGACCGCTACGTGCTGTTCGACATCAACTTCGCGCCGCTCGTCGAGGCGCTCGCGC
CGCAGTGTCCGAACGTGCGCGGCTGGATCGTCATGACCGACGTCGACCATCTGCCGCGCGGCGCGATGCCGTACCTGTGC
TACGAGACGCTCGTCGAGGCGGAGGACGGCCGCTACGACTGGCCGCGTCTCGACGAGCAGCAGGCTTGCGGGCTCTGCTA
CACGTCGGGCACGACGGGCAATCCGAAGGGCGTGCTGTATTCGAATCGCTCGACCGTGCTGCACGCATACGGCGCCGCGC
TGCCCGACGCGATGAACCTGTCGGCGATGGACGCCGTGCTGCCGGTCGTGCCGATGTTCCACGTCAACGCATGGGGGCTG
CCGTACGCGGTGCCGCTGACGGGCGGCAAGCTCGTGCTGCCGGGCAAGGATCTCGACGGCCGATCGCTCTACGAGCTGAT
GGAGGCCGAGCGCGTGACGTTTTCGGCGGGCGTGCCGACCGTGTGGCTCGGGTTGCTCAACTACATGCGCGAGGCGGGCG
TGCGCTTCTCGTCGCTGAACCGCACGGTGATCGGCGGCTCGGCCTGCCCGCCCGCGATGCTGCGCACGTTCGAGGACGAC
TACGGCGTGCGCGTGATCCACGCGTGGGGCATGACCGAGCTGTCGCCGCTCGGCACGCTCGCGAAGCTGAACTGGGCGCA
GTCCCAGCGGCCGCACGACGTGCAGCGGCGGCTGCTCGAAAAGCAGGGGCGCGTGATCGGCGGCGTCGACATGCGGATCG
TCGGCCCGGACGGACAGGAGCTGCCGTGGGACGGCGTCGCGTACGGCGAGCTGCAGGTGCGCGGGCCGTGGGTGATCGAC
CGCTACTTTCGCAGCGACACCTCGCCACTCGTCGACGGCTGGTTTCCGACCGGCGACGTCGCGACGATCGACGCGGACGG
CTTCCTGCAGATCACCGACCGCAGCAAGGACGTGATCAAGTCGGGCGGCGAATGGATCAGCTCGATCGACGTCGAAAACG
TCGCGATCGCACACCCGGGCGTCGCGGAGGCCGCCTGCATCGCATGCGCGCATCCGAAGTGGACCGAGCGGCCACTGCTC
GTCGTCGTGCCGCGCGAAGGCGCGAACCTGACGCGCGAGGCGCTGCTCGCGTTCTACGAAGGCAAGGTCGCGAAATGGTG
GATTCCGGACGACGTCGTGTTCGTCGAATCGCTGCCGCATACCGCGACCGGCAAGCTGCAGAAGCTGAAGCTGCGCGAAA
TGTTCCGCGACTACGTGCTGCCGACGGCGGTCTGCGCGCCGTAA

Upstream 100 bases:

>100_bases
GCATGGCGGCCCGCGCGATGCACGCCGCCCGCCCGGGCCGCCGCGCGCCGCCGTCGCGGGGCCCGTCACCGATTCGCGTC
CTTCAGGGGAGAACAAGACG

Downstream 100 bases:

>100_bases
CGCGGGCGCGGCCGGCCCTTCGGTCGGCCGCTGTTGCCGGCCGTCGCCGTTGCCGGTCGTCCTTGCCAGTCCCTTTGCCG
GTCGCCCTTGCCGGTCGCCT

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MGKPLLGQMMDMPLLVSSLIAHAARHAGDTEIVSKRIEGDLHRYTYRDCERRAKRLAQALARLGVEAGDRVGTLAWNGYR
HLEAYYAIGGMGAVCHTINPRLFPEQIAYIVNHAEDRYVLFDINFAPLVEALAPQCPNVRGWIVMTDVDHLPRGAMPYLC
YETLVEAEDGRYDWPRLDEQQACGLCYTSGTTGNPKGVLYSNRSTVLHAYGAALPDAMNLSAMDAVLPVVPMFHVNAWGL
PYAVPLTGGKLVLPGKDLDGRSLYELMEAERVTFSAGVPTVWLGLLNYMREAGVRFSSLNRTVIGGSACPPAMLRTFEDD
YGVRVIHAWGMTELSPLGTLAKLNWAQSQRPHDVQRRLLEKQGRVIGGVDMRIVGPDGQELPWDGVAYGELQVRGPWVID
RYFRSDTSPLVDGWFPTGDVATIDADGFLQITDRSKDVIKSGGEWISSIDVENVAIAHPGVAEAACIACAHPKWTERPLL
VVVPREGANLTREALLAFYEGKVAKWWIPDDVVFVESLPHTATGKLQKLKLREMFRDYVLPTAVCAP

Sequences:

>Translated_547_residues
MGKPLLGQMMDMPLLVSSLIAHAARHAGDTEIVSKRIEGDLHRYTYRDCERRAKRLAQALARLGVEAGDRVGTLAWNGYR
HLEAYYAIGGMGAVCHTINPRLFPEQIAYIVNHAEDRYVLFDINFAPLVEALAPQCPNVRGWIVMTDVDHLPRGAMPYLC
YETLVEAEDGRYDWPRLDEQQACGLCYTSGTTGNPKGVLYSNRSTVLHAYGAALPDAMNLSAMDAVLPVVPMFHVNAWGL
PYAVPLTGGKLVLPGKDLDGRSLYELMEAERVTFSAGVPTVWLGLLNYMREAGVRFSSLNRTVIGGSACPPAMLRTFEDD
YGVRVIHAWGMTELSPLGTLAKLNWAQSQRPHDVQRRLLEKQGRVIGGVDMRIVGPDGQELPWDGVAYGELQVRGPWVID
RYFRSDTSPLVDGWFPTGDVATIDADGFLQITDRSKDVIKSGGEWISSIDVENVAIAHPGVAEAACIACAHPKWTERPLL
VVVPREGANLTREALLAFYEGKVAKWWIPDDVVFVESLPHTATGKLQKLKLREMFRDYVLPTAVCAP
>Mature_546_residues
GKPLLGQMMDMPLLVSSLIAHAARHAGDTEIVSKRIEGDLHRYTYRDCERRAKRLAQALARLGVEAGDRVGTLAWNGYRH
LEAYYAIGGMGAVCHTINPRLFPEQIAYIVNHAEDRYVLFDINFAPLVEALAPQCPNVRGWIVMTDVDHLPRGAMPYLCY
ETLVEAEDGRYDWPRLDEQQACGLCYTSGTTGNPKGVLYSNRSTVLHAYGAALPDAMNLSAMDAVLPVVPMFHVNAWGLP
YAVPLTGGKLVLPGKDLDGRSLYELMEAERVTFSAGVPTVWLGLLNYMREAGVRFSSLNRTVIGGSACPPAMLRTFEDDY
GVRVIHAWGMTELSPLGTLAKLNWAQSQRPHDVQRRLLEKQGRVIGGVDMRIVGPDGQELPWDGVAYGELQVRGPWVIDR
YFRSDTSPLVDGWFPTGDVATIDADGFLQITDRSKDVIKSGGEWISSIDVENVAIAHPGVAEAACIACAHPKWTERPLLV
VVPREGANLTREALLAFYEGKVAKWWIPDDVVFVESLPHTATGKLQKLKLREMFRDYVLPTAVCAP

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=527, Percent_Identity=26.7552182163188, Blast_Score=169, Evalue=7e-42,
Organism=Homo sapiens, GI187761345, Length=526, Percent_Identity=27.1863117870722, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI187761343, Length=526, Percent_Identity=27.1863117870722, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI28416953, Length=568, Percent_Identity=24.8239436619718, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI38505220, Length=510, Percent_Identity=23.921568627451, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI115511026, Length=517, Percent_Identity=21.4700193423598, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI122937307, Length=511, Percent_Identity=22.7005870841487, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI58082049, Length=544, Percent_Identity=22.4264705882353, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI157311624, Length=546, Percent_Identity=22.1611721611722, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI157311622, Length=546, Percent_Identity=22.1611721611722, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788107, Length=526, Percent_Identity=26.2357414448669, Blast_Score=149, Evalue=7e-37,
Organism=Escherichia coli, GI221142682, Length=524, Percent_Identity=24.8091603053435, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI145693145, Length=500, Percent_Identity=26.4, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789201, Length=372, Percent_Identity=26.3440860215054, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1786810, Length=537, Percent_Identity=24.9534450651769, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=562, Percent_Identity=25.4448398576512, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71994703, Length=562, Percent_Identity=25.4448398576512, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71994694, Length=562, Percent_Identity=25.4448398576512, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17558820, Length=523, Percent_Identity=25.8126195028681, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=512, Percent_Identity=25.9765625, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=539, Percent_Identity=25.0463821892393, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=540, Percent_Identity=22.5925925925926, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=540, Percent_Identity=23.8888888888889, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=519, Percent_Identity=21.9653179190751, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=524, Percent_Identity=24.8091603053435, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=500, Percent_Identity=21.8, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71996755, Length=504, Percent_Identity=22.6190476190476, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=383, Percent_Identity=23.4986945169713, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=364, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320852, Length=438, Percent_Identity=25.5707762557078, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24581924, Length=548, Percent_Identity=26.8248175182482, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI18859661, Length=476, Percent_Identity=26.2605042016807, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21355181, Length=531, Percent_Identity=22.7871939736347, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922652, Length=508, Percent_Identity=24.0157480314961, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24656500, Length=498, Percent_Identity=25.5020080321285, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356441, Length=509, Percent_Identity=22.9862475442043, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648260, Length=513, Percent_Identity=25.1461988304094, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648257, Length=361, Percent_Identity=24.9307479224377, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648253, Length=551, Percent_Identity=21.7785843920145, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21358303, Length=470, Percent_Identity=23.6170212765957, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355225, Length=591, Percent_Identity=22.165820642978, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648255, Length=551, Percent_Identity=21.7785843920145, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281365686, Length=548, Percent_Identity=21.1678832116788, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24653035, Length=500, Percent_Identity=20.2, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21356947, Length=499, Percent_Identity=23.4468937875752, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI62472339, Length=577, Percent_Identity=22.7036395147314, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24667959, Length=405, Percent_Identity=22.962962962963, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24667955, Length=407, Percent_Identity=23.5872235872236, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI161076582, Length=509, Percent_Identity=20.6286836935167, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60467; Mature: 60336

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00139 THIOL_PROTEASE_CYS ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPLLGQMMDMPLLVSSLIAHAARHAGDTEIVSKRIEGDLHRYTYRDCERRAKRLAQAL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ARLGVEAGDRVGTLAWNGYRHLEAYYAIGGMGAVCHTINPRLFPEQIAYIVNHAEDRYVL
HHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHCCCCCEEE
FDINFAPLVEALAPQCPNVRGWIVMTDVDHLPRGAMPYLCYETLVEAEDGRYDWPRLDEQ
EECCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCH
QACGLCYTSGTTGNPKGVLYSNRSTVLHAYGAALPDAMNLSAMDAVLPVVPMFHVNAWGL
HHCCEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEECCCCC
PYAVPLTGGKLVLPGKDLDGRSLYELMEAERVTFSAGVPTVWLGLLNYMREAGVRFSSLN
CEEEEECCCEEEECCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCEEHHCC
RTVIGGSACPPAMLRTFEDDYGVRVIHAWGMTELSPLGTLAKLNWAQSQRPHDVQRRLLE
CEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCHHCCCCHHHHHHHHH
KQGRVIGGVDMRIVGPDGQELPWDGVAYGELQVRGPWVIDRYFRSDTSPLVDGWFPTGDV
HCCCEECCCEEEEECCCCCCCCCCCEEEEEEEECCCEEEHHHHHCCCCCCCCCCCCCCCE
ATIDADGFLQITDRSKDVIKSGGEWISSIDVENVAIAHPGVAEAACIACAHPKWTERPLL
EEECCCCEEEEECCCHHHHHCCCHHHHHCCCCCEEECCCCCHHHHHEEECCCCCCCCCEE
VVVPREGANLTREALLAFYEGKVAKWWIPDDVVFVESLPHTATGKLQKLKLREMFRDYVL
EEECCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCC
PTAVCAP
CHHHCCC
>Mature Secondary Structure 
GKPLLGQMMDMPLLVSSLIAHAARHAGDTEIVSKRIEGDLHRYTYRDCERRAKRLAQAL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ARLGVEAGDRVGTLAWNGYRHLEAYYAIGGMGAVCHTINPRLFPEQIAYIVNHAEDRYVL
HHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHCCCCCEEE
FDINFAPLVEALAPQCPNVRGWIVMTDVDHLPRGAMPYLCYETLVEAEDGRYDWPRLDEQ
EECCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCH
QACGLCYTSGTTGNPKGVLYSNRSTVLHAYGAALPDAMNLSAMDAVLPVVPMFHVNAWGL
HHCCEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEECCCCC
PYAVPLTGGKLVLPGKDLDGRSLYELMEAERVTFSAGVPTVWLGLLNYMREAGVRFSSLN
CEEEEECCCEEEECCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCEEHHCC
RTVIGGSACPPAMLRTFEDDYGVRVIHAWGMTELSPLGTLAKLNWAQSQRPHDVQRRLLE
CEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCHHCCCCHHHHHHHHH
KQGRVIGGVDMRIVGPDGQELPWDGVAYGELQVRGPWVIDRYFRSDTSPLVDGWFPTGDV
HCCCEECCCEEEEECCCCCCCCCCCEEEEEEEECCCEEEHHHHHCCCCCCCCCCCCCCCE
ATIDADGFLQITDRSKDVIKSGGEWISSIDVENVAIAHPGVAEAACIACAHPKWTERPLL
EEECCCCEEEEECCCHHHHHCCCHHHHHCCCCCEEECCCCCHHHHHEEECCCCCCCCCEE
VVVPREGANLTREALLAFYEGKVAKWWIPDDVVFVESLPHTATGKLQKLKLREMFRDYVL
EEECCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCC
PTAVCAP
CHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]