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The map label for this gene is rluE [H]

Identifier: 161523949

GI number: 161523949

Start: 842001

End: 842687

Strand: Reverse

Name: rluE [H]

Synonym: Bmul_0769

Alternate gene names: 161523949

Gene position: 842687-842001 (Counterclockwise)

Preceding gene: 161523952

Following gene: 161523948

Centisome position: 24.44

GC content: 67.83

Gene sequence:

>687_bases
ATGTCTTATATAAGACAGAGGACTTGGCGTTCCGTATTATGCATTAAGATTCCGCCATTTGCCATAGAGCGCCCGCCGCC
CCGCGCGCCGCGGGCCTGCGTCTCGCCCATGACCCTGATCGCCCTCAACAAGCCGTTCGGCACGATTTGCCAGTTTTCCG
CGCACGAAACGCGGCCGTCGCTCGGCGACTGGGTAAAAACGCCCGGCGTCTATCCGGCCGGCCGCCTCGACGCGGACAGC
GAAGGCCTGCTGCTGCTCACCGACGACGGCGCGCTGCAGGCGCGCATCGCGGAGCCGCGCCACAAGCTCGTGAAGCGCTA
CTGGGCGCAGGTCGAGGGCGCGCCGGGCCCGGCCGACCTGAAAGCGCTCGCGCGCGGCGTCGATCTCGGCGATTACGTGA
CGCGCCCGTGCCGCGCCGAATTCATCGAACCGCCCGAGACGCTATGGCCGCGCAACCCGCCGATCCGCTACCGCGCGGCG
ATCCCCACGACCTGGATCGAACTCGCGATCACCGAAGGCAAGAACCGGCAGGTACGCAGGATGACGGCGGCGGTCGGCTT
CCCGACCTTGCGCCTCGTGCGTGTCGGCATCGGCTCGCTCGACATATTCGCGCTCGGCATTGCACCGAGCGAAACAATCG
CGCTGCCGCCGCGCGCGCCGTGGGACGGTTTCGCACGCGAAGCCTGA

Upstream 100 bases:

>100_bases
CAAGGCCTGAGCTCCCCGGCGCCGACAGCGAGCCGCTATTCTAGCGCCCCAACCGGGCACGGCGGCACGAAGCGCGGTGC
TGCGTATCAAGTCTTCACTT

Downstream 100 bases:

>100_bases
GCGGCACGACGCGCGCGCACTTTTCCTCATCTTTTTCGTCAACCGCCTGCGAAGATCGCGCGTTAAACGACGCAGATGCC
ACCCTGAGCTATCCGGCATT

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSYIRQRTWRSVLCIKIPPFAIERPPPRAPRACVSPMTLIALNKPFGTICQFSAHETRPSLGDWVKTPGVYPAGRLDADS
EGLLLLTDDGALQARIAEPRHKLVKRYWAQVEGAPGPADLKALARGVDLGDYVTRPCRAEFIEPPETLWPRNPPIRYRAA
IPTTWIELAITEGKNRQVRRMTAAVGFPTLRLVRVGIGSLDIFALGIAPSETIALPPRAPWDGFAREA

Sequences:

>Translated_228_residues
MSYIRQRTWRSVLCIKIPPFAIERPPPRAPRACVSPMTLIALNKPFGTICQFSAHETRPSLGDWVKTPGVYPAGRLDADS
EGLLLLTDDGALQARIAEPRHKLVKRYWAQVEGAPGPADLKALARGVDLGDYVTRPCRAEFIEPPETLWPRNPPIRYRAA
IPTTWIELAITEGKNRQVRRMTAAVGFPTLRLVRVGIGSLDIFALGIAPSETIALPPRAPWDGFAREA
>Mature_227_residues
SYIRQRTWRSVLCIKIPPFAIERPPPRAPRACVSPMTLIALNKPFGTICQFSAHETRPSLGDWVKTPGVYPAGRLDADSE
GLLLLTDDGALQARIAEPRHKLVKRYWAQVEGAPGPADLKALARGVDLGDYVTRPCRAEFIEPPETLWPRNPPIRYRAAI
PTTWIELAITEGKNRQVRRMTAAVGFPTLRLVRVGIGSLDIFALGIAPSETIALPPRAPWDGFAREA

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=49.2227979274611, Blast_Score=181, Evalue=3e-47,
Organism=Escherichia coli, GI1788510, Length=175, Percent_Identity=33.7142857142857, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 25237; Mature: 25105

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYIRQRTWRSVLCIKIPPFAIERPPPRAPRACVSPMTLIALNKPFGTICQFSAHETRPS
CCHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHCCEEEEEECCCCHHHEECCCCCCCCC
LGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQARIAEPRHKLVKRYWAQVEGAPGPADL
CHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHH
KALARGVDLGDYVTRPCRAEFIEPPETLWPRNPPIRYRAAIPTTWIELAITEGKNRQVRR
HHHHHCCCCHHHHCCCCHHHCCCCCHHHCCCCCCEEEEEECCCEEEEEEEECCCCCHHHH
MTAAVGFPTLRLVRVGIGSLDIFALGIAPSETIALPPRAPWDGFAREA
HHHHHCCCHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SYIRQRTWRSVLCIKIPPFAIERPPPRAPRACVSPMTLIALNKPFGTICQFSAHETRPS
CHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHCCEEEEEECCCCHHHEECCCCCCCCC
LGDWVKTPGVYPAGRLDADSEGLLLLTDDGALQARIAEPRHKLVKRYWAQVEGAPGPADL
CHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHH
KALARGVDLGDYVTRPCRAEFIEPPETLWPRNPPIRYRAAIPTTWIELAITEGKNRQVRR
HHHHHCCCCHHHHCCCCHHHCCCCCHHHCCCCCCEEEEEECCCEEEEEEEECCCCCHHHH
MTAAVGFPTLRLVRVGIGSLDIFALGIAPSETIALPPRAPWDGFAREA
HHHHHCCCHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]