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The map label for this gene is citZ [H]

Identifier: 161523941

GI number: 161523941

Start: 832808

End: 834040

Strand: Reverse

Name: citZ [H]

Synonym: Bmul_0761

Alternate gene names: 161523941

Gene position: 834040-832808 (Counterclockwise)

Preceding gene: 161523943

Following gene: 161523939

Centisome position: 24.19

GC content: 75.51

Gene sequence:

>1233_bases
ATGACGTCTCTCAGCGCGCCCGAAGCGGCCGATCAGCTCGGCATCAGCGTGAGCACGCTCTATGCATACGTGAGCCGCGG
GCTGCTGCGCTCGCTGCCGGACGGCGCCGGCAAGCGGCGTCGCTACGACGCCGACGAAGTGCGCCTGCTCGCGCGCCGGC
GTGCCGATGCGAAACGGGCGGGCGGCGTCGCGGAGCGTTCGCTCGACTGGGGCGTGCCCGTGCTCGAATCGCGGATCACG
CAGATCGCCGACGGCCGCCTGCGCTACCGCGGCGCCGACGCGATCGCACTCGCGAACGACGCGACGCTCGAAGCGACCGC
CGCGCGGCTCTGGGACTGCGCACCGGCGCGGCTCGCGGCCGCCTCGCTCGATGCGAGCGGCTTCGATCGCGCGCAATGGG
ACGACTGGGCGCGCCGCTGGGCGCATCTCGCGCCGCTCGACCGCGCGCTCGTGCTGCTGCCGGCTGCCGCCGCGTCGCTG
CCGCGACTGTGGGCGCAGGAGCGCGACGCGCAGCTCGATTCGGCCGCCCTGCTGCTGCGAGTCGCGGCCGCCGCACTGGC
CGGCCGTGCACCGTCCGACGCGCCCGTGCATCGGCAACTCGCCGCTGCCTGGCGCGTGCGTCGACGCGATCACACCGATT
TGCTGCGCCGCGCGCTCGTGCTCTGCGCGGACCACGAACTGAACCCGTCGACGTTCGCGGTGCGCTGCATCGCGTCGACC
GGCACGCACCTGTTCGGCGCGATCGCGGGCGGGCTCGCCGCGCTATCGGGACCGCGCCACGGCGGCGAGACGTTCCGCGC
GGCGACGCTGCTCGACGAAGCGGCGCGCGCCGCCGATCTCGACCGCTACCTCGCGCTGCGGATCGCGCACGACGAACGCG
CGGACGGCGGCCGCACCGCGCTGGCCGGCTTCGGGCACCGGCTGTATCCGGATGGCGATCCGCGCGCGCGGGCGCTGCTC
GACGCATTGCACGCGGCGCTGCCGCAGCGGCCGGCCCTGCGCGCGGCGCGCACGCTGGCCGCACGCGTCGAAGCGGCGAC
CGGGCTCCAGCCGACGATCGACTTTGCGCTCGCGGCGCTCGAACGCGCACTCGCGCTGCCGGACGGCGCGGCGTTCACGC
TGTTCGCGGCGGGCCGCACGGCCGGCTGGATCGCGCATGCGCTCGAACAGTCGGCGAACGGGAAGCTGATCCGCCCGCGC
GCACGCTACGTCGGCAACGACGCGGCCGCGTGA

Upstream 100 bases:

>100_bases
AATTCGCCACACACGCGATCAATCTTGATTGCGCGAATCAATACATAACGAATAGTTGTAGCGCGAGCGCGCCTTATGCG
CGCCGACAGGAGCGATCGAC

Downstream 100 bases:

>100_bases
TGCCGTGCGTCGGTCGCGTGCCGCACCGCCGGCGCGACGCGCGCAACGGGCGCGCGATGCGGCCCGAACGCGAACGTCGC
GCCGCCGCGCTCGGCGCCGA

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTSLSAPEAADQLGISVSTLYAYVSRGLLRSLPDGAGKRRRYDADEVRLLARRRADAKRAGGVAERSLDWGVPVLESRIT
QIADGRLRYRGADAIALANDATLEATAARLWDCAPARLAAASLDASGFDRAQWDDWARRWAHLAPLDRALVLLPAAAASL
PRLWAQERDAQLDSAALLLRVAAAALAGRAPSDAPVHRQLAAAWRVRRRDHTDLLRRALVLCADHELNPSTFAVRCIAST
GTHLFGAIAGGLAALSGPRHGGETFRAATLLDEAARAADLDRYLALRIAHDERADGGRTALAGFGHRLYPDGDPRARALL
DALHAALPQRPALRAARTLAARVEAATGLQPTIDFALAALERALALPDGAAFTLFAAGRTAGWIAHALEQSANGKLIRPR
ARYVGNDAAA

Sequences:

>Translated_410_residues
MTSLSAPEAADQLGISVSTLYAYVSRGLLRSLPDGAGKRRRYDADEVRLLARRRADAKRAGGVAERSLDWGVPVLESRIT
QIADGRLRYRGADAIALANDATLEATAARLWDCAPARLAAASLDASGFDRAQWDDWARRWAHLAPLDRALVLLPAAAASL
PRLWAQERDAQLDSAALLLRVAAAALAGRAPSDAPVHRQLAAAWRVRRRDHTDLLRRALVLCADHELNPSTFAVRCIAST
GTHLFGAIAGGLAALSGPRHGGETFRAATLLDEAARAADLDRYLALRIAHDERADGGRTALAGFGHRLYPDGDPRARALL
DALHAALPQRPALRAARTLAARVEAATGLQPTIDFALAALERALALPDGAAFTLFAAGRTAGWIAHALEQSANGKLIRPR
ARYVGNDAAA
>Mature_409_residues
TSLSAPEAADQLGISVSTLYAYVSRGLLRSLPDGAGKRRRYDADEVRLLARRRADAKRAGGVAERSLDWGVPVLESRITQ
IADGRLRYRGADAIALANDATLEATAARLWDCAPARLAAASLDASGFDRAQWDDWARRWAHLAPLDRALVLLPAAAASLP
RLWAQERDAQLDSAALLLRVAAAALAGRAPSDAPVHRQLAAAWRVRRRDHTDLLRRALVLCADHELNPSTFAVRCIASTG
THLFGAIAGGLAALSGPRHGGETFRAATLLDEAARAADLDRYLALRIAHDERADGGRTALAGFGHRLYPDGDPRARALLD
ALHAALPQRPALRAARTLAARVEAATGLQPTIDFALAALERALALPDGAAFTLFAAGRTAGWIAHALEQSANGKLIRPRA
RYVGNDAAA

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786939, Length=203, Percent_Identity=34.9753694581281, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786527, Length=195, Percent_Identity=31.2820512820513, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 43696; Mature: 43565

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLSAPEAADQLGISVSTLYAYVSRGLLRSLPDGAGKRRRYDADEVRLLARRRADAKRA
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GGVAERSLDWGVPVLESRITQIADGRLRYRGADAIALANDATLEATAARLWDCAPARLAA
CCCHHHCCCCCCHHHHHHHHHHHCCCEEECCCCEEEECCCCHHHHHHHHHHCCCHHHHHH
ASLDASGFDRAQWDDWARRWAHLAPLDRALVLLPAAAASLPRLWAQERDAQLDSAALLLR
HHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
VAAAALAGRAPSDAPVHRQLAAAWRVRRRDHTDLLRRALVLCADHELNPSTFAVRCIAST
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHHC
GTHLFGAIAGGLAALSGPRHGGETFRAATLLDEAARAADLDRYLALRIAHDERADGGRTA
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHH
LAGFGHRLYPDGDPRARALLDALHAALPQRPALRAARTLAARVEAATGLQPTIDFALAAL
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ERALALPDGAAFTLFAAGRTAGWIAHALEQSANGKLIRPRARYVGNDAAA
HHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEECCHHHHCCCCCCC
>Mature Secondary Structure 
TSLSAPEAADQLGISVSTLYAYVSRGLLRSLPDGAGKRRRYDADEVRLLARRRADAKRA
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GGVAERSLDWGVPVLESRITQIADGRLRYRGADAIALANDATLEATAARLWDCAPARLAA
CCCHHHCCCCCCHHHHHHHHHHHCCCEEECCCCEEEECCCCHHHHHHHHHHCCCHHHHHH
ASLDASGFDRAQWDDWARRWAHLAPLDRALVLLPAAAASLPRLWAQERDAQLDSAALLLR
HHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
VAAAALAGRAPSDAPVHRQLAAAWRVRRRDHTDLLRRALVLCADHELNPSTFAVRCIAST
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHHC
GTHLFGAIAGGLAALSGPRHGGETFRAATLLDEAARAADLDRYLALRIAHDERADGGRTA
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHH
LAGFGHRLYPDGDPRARALLDALHAALPQRPALRAARTLAARVEAATGLQPTIDFALAAL
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ERALALPDGAAFTLFAAGRTAGWIAHALEQSANGKLIRPRARYVGNDAAA
HHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEECCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9006016; 12397186 [H]