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The map label for this gene is amiC [H]

Identifier: 161523913

GI number: 161523913

Start: 805639

End: 807156

Strand: Reverse

Name: amiC [H]

Synonym: Bmul_0733

Alternate gene names: 161523913

Gene position: 807156-805639 (Counterclockwise)

Preceding gene: 161523914

Following gene: 161523911

Centisome position: 23.41

GC content: 68.77

Gene sequence:

>1518_bases
ATGTCTCGAAAGATGTTGATCAAACCGTTCCGCTCGATCGAATCGGCGGCCACCGCGACGCACAACTGGCGGCGCCGGCA
GATCCTGTGCGCGGGCGCCTCGACGCTGGTGCTCGGCCTCGTCGCGCCCCGGCTCGCGCACGCGTCGTCGGTGCTCGGCG
TGCGCGTGTGGCCCGCACGCGATTACACGCGCGTGACGATCGAATCCGATCAGCCGCTGCAGAATAGCCAACAGCTGCTG
CAGGGGCCCGACCGGCTCGTCGTCGACCTGACCGGCCTCGAGCTCGACCAGGCGCTGCGCGACCTCGTGTCGAAGATCGC
GCCGAACGATCCGCAGATTCAATCGGTGCGCGTCGGCCAGTATCAGCCGCACGTCGTGCGGATGGTGTTCGACCTGAAAG
GCTCGGTGAAGCCGCAGGTGTTCACGCTGCCGCCGGTCGGCACCTACAAGTATCGGCTCGTGTTCGACCTCTATCCGGCC
GTCGCGCCCGATCCGCTGACCGACCTGATCGCGCAGACCGAACGCAAGGAGCAGGCGCTGAACGATGCGGCGCGCGCGCA
GCAGATGCAGCCGCCGACCGCGCTGAACGGCCCGGCGGCCGCGCCCGCGCCGAGCGACAACAGCGACGCGTTTTTCCAGC
GCTTCGCGCAGAACACGCCGGCCGCGCCGCACACGCCGCCGGCCGCGCCTTCGGCGCCCGCGAAGCCGGCCGTCAAGCCG
CCGCCCGTGATCGCGCGCCGCGACGACGGCGACGACGACGCCGATGCCTACAAGTTCACCGCGCCGAAGTCCGGCAAGGG
CGGCACCGTGCGGCTGCTGACCGTCGCGATCGATCCGGGCCACGGCGGCGAGGATCCGGGCGCGATCGGCGGCGCCGGCA
CGTACGAGAAGCACATCGCGCTCGACATCGCGAAGAAGCTGCGCGCGAAGATCGATGGCGCGCCGAACATGCGCGCGATG
ATGACGCGCGACGCCGACTTCTTCGTGCCGCTGAACGTGCGCGTGCAAAAGGCGCGCCGCGTCGGCGCCGACCTGTTCGT
GTCGATCCACGCCGATGCATTCACGACGCCGTCCGCGCGCGGCTCGTCGGTGTTCGCGCTGTCGGATCACGGCGCGTCGA
GCGCGGCCGCGCGCTGGCTCGCGAACAAGGAGAATGCGTCCGATCTGATCGGCGGGATCAACATCAAGACGCAGGACGCG
GCGGTGAGCCGCGCGCTGTTCGACATGTCGACCACGGCGCAGATTCGCGATTCGCTGCGCTACGGCAACTACGTGCTGAA
GGAAGTCGGCAGCATCAACAAGCTGCACAAGGGCTCGGTCGAGCAGGCGGGATTCGCGGTGCTGAAGGCGCCCGACATTC
CGTCGATCCTCGTCGAGACCGCGTTCATCAGCAACCCGGACGAGGAGCGCCGGCTCAACGACGACAGCTATCGCGACCAG
ATGGCCGACGCGATCTTCCGCGGCATCAAGCGCTACTTCGCCGCGAATCCGCCGCTCGCGAAGAACCGCATGGCCTGA

Upstream 100 bases:

>100_bases
CGGGCACCCTGCTCGGCGTGCCCGATCTGGTCTTCTCGCTCGACGTGGACGGCGACGGCCGCGCCCTCACCGTCCGGGCG
TATAGCGCTTCAGGAAACGC

Downstream 100 bases:

>100_bases
CGGCCGCATCGCCGCCGGCACGCCACGCGCGTGCCGGCGCGCCTCACGACGCCGCGAAGCGGCGCATCAGCTTCCCGCCG
AACACGTTCACCGCGAGCCC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSRKMLIKPFRSIESAATATHNWRRRQILCAGASTLVLGLVAPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNSQQLL
QGPDRLVVDLTGLELDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPA
VAPDPLTDLIAQTERKEQALNDAARAQQMQPPTALNGPAAAPAPSDNSDAFFQRFAQNTPAAPHTPPAAPSAPAKPAVKP
PPVIARRDDGDDDADAYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGAGTYEKHIALDIAKKLRAKIDGAPNMRAM
MTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGASSAAARWLANKENASDLIGGINIKTQDA
AVSRALFDMSTTAQIRDSLRYGNYVLKEVGSINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERRLNDDSYRDQ
MADAIFRGIKRYFAANPPLAKNRMA

Sequences:

>Translated_505_residues
MSRKMLIKPFRSIESAATATHNWRRRQILCAGASTLVLGLVAPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNSQQLL
QGPDRLVVDLTGLELDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPA
VAPDPLTDLIAQTERKEQALNDAARAQQMQPPTALNGPAAAPAPSDNSDAFFQRFAQNTPAAPHTPPAAPSAPAKPAVKP
PPVIARRDDGDDDADAYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGAGTYEKHIALDIAKKLRAKIDGAPNMRAM
MTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGASSAAARWLANKENASDLIGGINIKTQDA
AVSRALFDMSTTAQIRDSLRYGNYVLKEVGSINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERRLNDDSYRDQ
MADAIFRGIKRYFAANPPLAKNRMA
>Mature_504_residues
SRKMLIKPFRSIESAATATHNWRRRQILCAGASTLVLGLVAPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNSQQLLQ
GPDRLVVDLTGLELDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAV
APDPLTDLIAQTERKEQALNDAARAQQMQPPTALNGPAAAPAPSDNSDAFFQRFAQNTPAAPHTPPAAPSAPAKPAVKPP
PVIARRDDGDDDADAYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGAGTYEKHIALDIAKKLRAKIDGAPNMRAMM
TRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGASSAAARWLANKENASDLIGGINIKTQDAA
VSRALFDMSTTAQIRDSLRYGNYVLKEVGSINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERRLNDDSYRDQM
ADAIFRGIKRYFAANPPLAKNRMA

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=479, Percent_Identity=41.3361169102296, Blast_Score=331, Evalue=8e-92,
Organism=Escherichia coli, GI1788776, Length=234, Percent_Identity=47.8632478632479, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1790611, Length=229, Percent_Identity=41.9213973799127, Blast_Score=188, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 54601; Mature: 54469

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKMLIKPFRSIESAATATHNWRRRQILCAGASTLVLGLVAPRLAHASSVLGVRVWPAR
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHEEEEEEECCC
DYTRVTIESDQPLQNSQQLLQGPDRLVVDLTGLELDQALRDLVSKIAPNDPQIQSVRVGQ
CCEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCC
YQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPDPLTDLIAQTERKEQAL
CCCHHEEEEECCCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
NDAARAQQMQPPTALNGPAAAPAPSDNSDAFFQRFAQNTPAAPHTPPAAPSAPAKPAVKP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PPVIARRDDGDDDADAYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGAGTYEKHIA
CCEEEECCCCCCCCCCEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHH
LDIAKKLRAKIDGAPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSAR
HHHHHHHHHHCCCCCCCEEEEECCCCEEEECCHHHHHHHHCCCEEEEEEECCCCCCCCCC
GSSVFALSDHGASSAAARWLANKENASDLIGGINIKTQDAAVSRALFDMSTTAQIRDSLR
CCEEEEEECCCCHHHHHHHHCCCCCHHHHHCCCCEEEHHHHHHHHHHHCCHHHHHHHHHH
YGNYVLKEVGSINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERRLNDDSYRDQ
HHHHHHHHHCCHHHHCCCCCHHCCEEEEECCCCCHHHHHHHHCCCCCHHHCCCCHHHHHH
MADAIFRGIKRYFAANPPLAKNRMA
HHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SRKMLIKPFRSIESAATATHNWRRRQILCAGASTLVLGLVAPRLAHASSVLGVRVWPAR
CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHEEEEEEECCC
DYTRVTIESDQPLQNSQQLLQGPDRLVVDLTGLELDQALRDLVSKIAPNDPQIQSVRVGQ
CCEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCC
YQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPDPLTDLIAQTERKEQAL
CCCHHEEEEECCCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
NDAARAQQMQPPTALNGPAAAPAPSDNSDAFFQRFAQNTPAAPHTPPAAPSAPAKPAVKP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PPVIARRDDGDDDADAYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGAGTYEKHIA
CCEEEECCCCCCCCCCEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHH
LDIAKKLRAKIDGAPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSAR
HHHHHHHHHHCCCCCCCEEEEECCCCEEEECCHHHHHHHHCCCEEEEEEECCCCCCCCCC
GSSVFALSDHGASSAAARWLANKENASDLIGGINIKTQDAAVSRALFDMSTTAQIRDSLR
CCEEEEEECCCCHHHHHHHHCCCCCHHHHHCCCCEEEHHHHHHHHHHHCCHHHHHHHHHH
YGNYVLKEVGSINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPDEERRLNDDSYRDQ
HHHHHHHHHCCHHHHCCCCCHHCCEEEEECCCCCHHHHHHHHCCCCCHHHCCCCHHHHHH
MADAIFRGIKRYFAANPPLAKNRMA
HHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]