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The map label for this gene is mdlC [H]

Identifier: 161523894

GI number: 161523894

Start: 785217

End: 786827

Strand: Direct

Name: mdlC [H]

Synonym: Bmul_0714

Alternate gene names: 161523894

Gene position: 785217-786827 (Clockwise)

Preceding gene: 161523889

Following gene: 161523895

Centisome position: 22.77

GC content: 72.13

Gene sequence:

>1611_bases
ATGTCCGGTCAACCGCACGCCGCCGCCACGCCCGCGACCACCGTTCGCGACGCCGTGATCCATCTGTTCCGCCAGTTCGG
CATCGACCGCGTATTCGGCAATCCCGGCTCGACCGAGCTGCCGATGTTCCGCGATTTCCCGGACGATTTCCGCTATGTGC
TCGGGCTGCAGGAGGCCGTCGTCGTCGGCATGGCCGACGGTTACGCGCAGGCGTCCGGCAATGCGGCGGTCGTCAACCTC
CATTCGGCGGCCGGCGTCGGCAATGCGATGGGCAACCTCTTCACCGCGTACAAGAACCGCACGCCGTTGATCGTTACCGC
CGGCCAGCAGGCGCGCGCGATCCTGCCGTTCGAGCCGTTCCTCGGCGCCGCGCAGGCCACCGAGCTGCCCAAGCCGTACG
TGAAATGGAGCATCGAGCCGGCGCGCGCGCAGGACGTGCCGGCCGCGATCGCGCGCGCGTACCGGATCGCGATGCAGGAG
CCGCGCGGGCCCGTGTTCGTGTCGATCCCGGTCGACGACTGGGATCAGCCCGCCGAACTGCTGCCGGCGCGCGAGGTGAG
CCAGGTCGTGCGGCCCGACCCCGACGCGCTCGCGCGGCTCGGCGACGCGCTCGATGCCGCGCAGCGTCCCGCGTTCGTCG
TCGGCGCGGCAATCGATCGTGCGGGCGCGTGGGACGAGGCCGTGCGGCTTGCCGAGCGGCATCGCGCACGCGTGTACGTC
GCGCCGATGTCGGGGCGCTGCAGCTTTCCCGAAGACCATCCGTTGTTCGCCGGCTTCCTGCCCGCGATTCGCGAGAAGAT
CGTCGCGCGGCTCGACGGGCACGACCTCGTGTTCGCGTTCGGCGCGCCCGCATTCACGTACCACATCGAGGGTTTCGGCC
CGCATGTGCCGCCCGGCGCGACGCTCGTGCAGCTCGTCGACGATCCGGGCACGGCCGCGTGGACGCCGCAAGGCGACGCG
GTCGTCGGCAATCTGAAGCTCGCGGCGCGCGATCTGCTCGCGCGGCCCGCGCCCGCCGAGCGGCCGATGCCGGCCGCGCG
CGCACCGCGCGAGCGCGTCGCACCGCCGGCCGCCGGCGAGCGCATGTCGGCCGCGTTCGTGCTGCAGACGCTCGCCGACC
TGCGCGATGCGCACGACATCGTCGTCGAGGAAGCGCCGAGCGCGCGGCCCATCATGCAGGCGCATCTGCCGTTCACGCAG
AGCGGCACGTTCTACACGATGGACAGCGGCGGGCTCGGCTACGGGATGCCGGCCGCCGTCGGCGTCGCACTGGCGCAGCC
GGGGCGGCGCGTGATCGGGCTGATCGGCGACGGCTCGAGCCTGTATTCGATCCAGGCGCTGTGGAGCGCCGCGCAGCTCA
GGCTGCCGATCACGTTCGTGATCCTCAACAACCGGCGCTACGCGGCGCTGCAGGATTTCGCGCCGGTCTTCGGCTTCGGG
CCGGACGATCCGGTGCAGGGCACCGATCTGCCCGATCTCGATTTCGTCGCGCTCGCCGCCGCGTTCGGCTGCCGCGGCGT
GCGCGTCGACGATCACGCGCGCCTGCGCGACACGCTGGCCGACGCGCTGCGTGCGACCGCGCCGGTCGTCGTCGACGTCG
AGATCGCCTGA

Upstream 100 bases:

>100_bases
ATGCGCAAACGGTATGGGTTTTCGCCAAAAATACAGTATCCGAGAATGGCCTGCACTTCGATACTGTGCGGATCCATCCG
TTCTCGAGGAGACGCCGCGC

Downstream 100 bases:

>100_bases
TGCGCGCCGCGCGTCCTTCCGGAGAAACCATGCAAACCGTATCGATGCTGATCGGCGGCGAACGCCGCCAATCGTCCAAC
GGCGCCAGCTTCGTGCGCCG

Product: benzoylformate decarboxylase

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSGQPHAAATPATTVRDAVIHLFRQFGIDRVFGNPGSTELPMFRDFPDDFRYVLGLQEAVVVGMADGYAQASGNAAVVNL
HSAAGVGNAMGNLFTAYKNRTPLIVTAGQQARAILPFEPFLGAAQATELPKPYVKWSIEPARAQDVPAAIARAYRIAMQE
PRGPVFVSIPVDDWDQPAELLPAREVSQVVRPDPDALARLGDALDAAQRPAFVVGAAIDRAGAWDEAVRLAERHRARVYV
APMSGRCSFPEDHPLFAGFLPAIREKIVARLDGHDLVFAFGAPAFTYHIEGFGPHVPPGATLVQLVDDPGTAAWTPQGDA
VVGNLKLAARDLLARPAPAERPMPAARAPRERVAPPAAGERMSAAFVLQTLADLRDAHDIVVEEAPSARPIMQAHLPFTQ
SGTFYTMDSGGLGYGMPAAVGVALAQPGRRVIGLIGDGSSLYSIQALWSAAQLRLPITFVILNNRRYAALQDFAPVFGFG
PDDPVQGTDLPDLDFVALAAAFGCRGVRVDDHARLRDTLADALRATAPVVVDVEIA

Sequences:

>Translated_536_residues
MSGQPHAAATPATTVRDAVIHLFRQFGIDRVFGNPGSTELPMFRDFPDDFRYVLGLQEAVVVGMADGYAQASGNAAVVNL
HSAAGVGNAMGNLFTAYKNRTPLIVTAGQQARAILPFEPFLGAAQATELPKPYVKWSIEPARAQDVPAAIARAYRIAMQE
PRGPVFVSIPVDDWDQPAELLPAREVSQVVRPDPDALARLGDALDAAQRPAFVVGAAIDRAGAWDEAVRLAERHRARVYV
APMSGRCSFPEDHPLFAGFLPAIREKIVARLDGHDLVFAFGAPAFTYHIEGFGPHVPPGATLVQLVDDPGTAAWTPQGDA
VVGNLKLAARDLLARPAPAERPMPAARAPRERVAPPAAGERMSAAFVLQTLADLRDAHDIVVEEAPSARPIMQAHLPFTQ
SGTFYTMDSGGLGYGMPAAVGVALAQPGRRVIGLIGDGSSLYSIQALWSAAQLRLPITFVILNNRRYAALQDFAPVFGFG
PDDPVQGTDLPDLDFVALAAAFGCRGVRVDDHARLRDTLADALRATAPVVVDVEIA
>Mature_535_residues
SGQPHAAATPATTVRDAVIHLFRQFGIDRVFGNPGSTELPMFRDFPDDFRYVLGLQEAVVVGMADGYAQASGNAAVVNLH
SAAGVGNAMGNLFTAYKNRTPLIVTAGQQARAILPFEPFLGAAQATELPKPYVKWSIEPARAQDVPAAIARAYRIAMQEP
RGPVFVSIPVDDWDQPAELLPAREVSQVVRPDPDALARLGDALDAAQRPAFVVGAAIDRAGAWDEAVRLAERHRARVYVA
PMSGRCSFPEDHPLFAGFLPAIREKIVARLDGHDLVFAFGAPAFTYHIEGFGPHVPPGATLVQLVDDPGTAAWTPQGDAV
VGNLKLAARDLLARPAPAERPMPAARAPRERVAPPAAGERMSAAFVLQTLADLRDAHDIVVEEAPSARPIMQAHLPFTQS
GTFYTMDSGGLGYGMPAAVGVALAQPGRRVIGLIGDGSSLYSIQALWSAAQLRLPITFVILNNRRYAALQDFAPVFGFGP
DDPVQGTDLPDLDFVALAAAFGCRGVRVDDHARLRDTLADALRATAPVVVDVEIA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=519, Percent_Identity=23.3140655105973, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI87081685, Length=539, Percent_Identity=27.6437847866419, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1790104, Length=536, Percent_Identity=24.2537313432836, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=24.5318352059925, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787096, Length=508, Percent_Identity=24.2125984251968, Blast_Score=85, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323755, Length=554, Percent_Identity=25.812274368231, Blast_Score=128, Evalue=3e-30,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 57135; Mature: 57003

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQPHAAATPATTVRDAVIHLFRQFGIDRVFGNPGSTELPMFRDFPDDFRYVLGLQEAV
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHE
VVGMADGYAQASGNAAVVNLHSAAGVGNAMGNLFTAYKNRTPLIVTAGQQARAILPFEPF
EEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCEECCCHHH
LGAAQATELPKPYVKWSIEPARAQDVPAAIARAYRIAMQEPRGPVFVSIPVDDWDQPAEL
CCCHHHHCCCCCHHEEECCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHH
LPAREVSQVVRPDPDALARLGDALDAAQRPAFVVGAAIDRAGAWDEAVRLAERHRARVYV
CCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCHHHHHHHHHHCCCEEEE
APMSGRCSFPEDHPLFAGFLPAIREKIVARLDGHDLVFAFGAPAFTYHIEGFGPHVPPGA
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCC
TLVQLVDDPGTAAWTPQGDAVVGNLKLAARDLLARPAPAERPMPAARAPRERVAPPAAGE
EEEEEECCCCCEEECCCCCEEEECHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCC
RMSAAFVLQTLADLRDAHDIVVEEAPSARPIMQAHLPFTQSGTFYTMDSGGLGYGMPAAV
HHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHEECCCCCCCCCEEEECCCCCCCCCHHHH
GVALAQPGRRVIGLIGDGSSLYSIQALWSAAQLRLPITFVILNNRRYAALQDFAPVFGFG
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHEECCEEEEEECCCCEEHHHHCCHHCCCC
PDDPVQGTDLPDLDFVALAAAFGCRGVRVDDHARLRDTLADALRATAPVVVDVEIA
CCCCCCCCCCCCHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure 
SGQPHAAATPATTVRDAVIHLFRQFGIDRVFGNPGSTELPMFRDFPDDFRYVLGLQEAV
CCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHE
VVGMADGYAQASGNAAVVNLHSAAGVGNAMGNLFTAYKNRTPLIVTAGQQARAILPFEPF
EEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCEECCCHHH
LGAAQATELPKPYVKWSIEPARAQDVPAAIARAYRIAMQEPRGPVFVSIPVDDWDQPAEL
CCCHHHHCCCCCHHEEECCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHH
LPAREVSQVVRPDPDALARLGDALDAAQRPAFVVGAAIDRAGAWDEAVRLAERHRARVYV
CCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCHHHHHHHHHHCCCEEEE
APMSGRCSFPEDHPLFAGFLPAIREKIVARLDGHDLVFAFGAPAFTYHIEGFGPHVPPGA
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCC
TLVQLVDDPGTAAWTPQGDAVVGNLKLAARDLLARPAPAERPMPAARAPRERVAPPAAGE
EEEEEECCCCCEEECCCCCEEEECHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCC
RMSAAFVLQTLADLRDAHDIVVEEAPSARPIMQAHLPFTQSGTFYTMDSGGLGYGMPAAV
HHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHEECCCCCCCCCEEEECCCCCCCCCHHHH
GVALAQPGRRVIGLIGDGSSLYSIQALWSAAQLRLPITFVILNNRRYAALQDFAPVFGFG
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHEECCEEEEEECCCCEEHHHHCCHHCCCC
PDDPVQGTDLPDLDFVALAAAFGCRGVRVDDHARLRDTLADALRATAPVVVDVEIA
CCCCCCCCCCCCHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]