Click here to switch to the map view.

The map label for this gene is ilvE [H]

Identifier: 161523847

GI number: 161523847

Start: 729863

End: 730786

Strand: Reverse

Name: ilvE [H]

Synonym: Bmul_0667

Alternate gene names: 161523847

Gene position: 730786-729863 (Counterclockwise)

Preceding gene: 161523857

Following gene: 161523846

Centisome position: 21.19

GC content: 64.5

Gene sequence:

>924_bases
ATGTCAATGGCCGACCGCGACGGCAAGATCTGGATGGACGGCAAGCTGATCGACTGGCGCGACGCCACGATCCACGTTCT
GACCCACACGCTGCACTACGGCATGGGCGTCTTCGAGGGCGTGCGCGCATACAAGACGGCCGACGGCAGCACCGCGATCT
TCCGTCTGCCCGAGCACACGAAACGTCTGCTGAATTCCGCGAAGATCTTCCAGATGGACGTGCCGTTCGACCAGGAAACG
CTGGAAGCCGCGCAGCGCGAAGTCGTACGCGAGAACAAGCTCGAGTCGTGCTACCTGCGCCCGATCATCTGGGTCGGCTC
GGAAAAGCTCGGCGTGTCGGCGAAGGGCAACACGATCCACGTCGCGATCGCCGCGTGGCCGTGGGGCGCGTACCTCGGCG
AGGACGGCCTCGCGAAGGGCATCCGCGTGAAGACGTCGTCGTTCACGCGCCATCACGTGAACGTGTCGATGGTGCGCGCG
AAGGCGTCGGGCTGGTACGTGAACTCGATCCTCGCGAACCAGGAAGCGACGGCCGACGGCTACGACGAAGCGCTGCTGCT
CGACGTCGACGGCTACGTGTCCGAAGGCTCCGGCGAGAACTTCTTCCTCGTGAACCGCGGCAAGCTGTACACGCCCGATC
TGTCGTCGTGCCTCGACGGCATCACGCGCGACACGATCATCACGCTCGCGAAGGACGCCGGCATCGAAGTCATCGAGAAG
CGCATCACGCGCGACGAGGTCTACACGGCCGACGAAGCGTTCTTCACCGGCACGGCCGCCGAAGTCACGCCGATCCGCGA
ACTCGACAACCGCACGATCGGCAGCGGCGCGCGCGGCCCCGTCACCGAAAAGCTGCAATCGGCGTTTTTCGATATCGTGT
CGGGCAAGAACGCGAAGTACGCGCACTGGCTGACCAAGGTCTGA

Upstream 100 bases:

>100_bases
CGCCGCACCTGCGCCGACCGACCGGCCCGCCCCCAAATGACGCTAAAATAAGCGTCTTTCCGGCTCAACGCTGCACACAA
CCGCATCCCAGGAGAATCCT

Downstream 100 bases:

>100_bases
GCGCGCGCGCCCCGACCGCCCCACCAAGAAGAGTGACAAGAGAAAGTCTCATGAGTGAAATCAAGGAAATGCCGCTGGTC
GAGCTGGCGGCGAAGGACCT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSMADRDGKIWMDGKLIDWRDATIHVLTHTLHYGMGVFEGVRAYKTADGSTAIFRLPEHTKRLLNSAKIFQMDVPFDQET
LEAAQREVVRENKLESCYLRPIIWVGSEKLGVSAKGNTIHVAIAAWPWGAYLGEDGLAKGIRVKTSSFTRHHVNVSMVRA
KASGWYVNSILANQEATADGYDEALLLDVDGYVSEGSGENFFLVNRGKLYTPDLSSCLDGITRDTIITLAKDAGIEVIEK
RITRDEVYTADEAFFTGTAAEVTPIRELDNRTIGSGARGPVTEKLQSAFFDIVSGKNAKYAHWLTKV

Sequences:

>Translated_307_residues
MSMADRDGKIWMDGKLIDWRDATIHVLTHTLHYGMGVFEGVRAYKTADGSTAIFRLPEHTKRLLNSAKIFQMDVPFDQET
LEAAQREVVRENKLESCYLRPIIWVGSEKLGVSAKGNTIHVAIAAWPWGAYLGEDGLAKGIRVKTSSFTRHHVNVSMVRA
KASGWYVNSILANQEATADGYDEALLLDVDGYVSEGSGENFFLVNRGKLYTPDLSSCLDGITRDTIITLAKDAGIEVIEK
RITRDEVYTADEAFFTGTAAEVTPIRELDNRTIGSGARGPVTEKLQSAFFDIVSGKNAKYAHWLTKV
>Mature_306_residues
SMADRDGKIWMDGKLIDWRDATIHVLTHTLHYGMGVFEGVRAYKTADGSTAIFRLPEHTKRLLNSAKIFQMDVPFDQETL
EAAQREVVRENKLESCYLRPIIWVGSEKLGVSAKGNTIHVAIAAWPWGAYLGEDGLAKGIRVKTSSFTRHHVNVSMVRAK
ASGWYVNSILANQEATADGYDEALLLDVDGYVSEGSGENFFLVNRGKLYTPDLSSCLDGITRDTIITLAKDAGIEVIEKR
ITRDEVYTADEAFFTGTAAEVTPIRELDNRTIGSGARGPVTEKLQSAFFDIVSGKNAKYAHWLTKV

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=334, Percent_Identity=26.9461077844311, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI48994963, Length=300, Percent_Identity=47.3333333333333, Blast_Score=280, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI17565728, Length=280, Percent_Identity=26.4285714285714, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322608, Length=285, Percent_Identity=27.719298245614, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6322002, Length=305, Percent_Identity=27.2131147540984, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24641779, Length=319, Percent_Identity=26.3322884012539, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34010; Mature: 33879

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMADRDGKIWMDGKLIDWRDATIHVLTHTLHYGMGVFEGVRAYKTADGSTAIFRLPEHT
CCCCCCCCEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHH
KRLLNSAKIFQMDVPFDQETLEAAQREVVRENKLESCYLRPIIWVGSEKLGVSAKGNTIH
HHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEECEEEEECCCCCCEEECCCEEE
VAIAAWPWGAYLGEDGLAKGIRVKTSSFTRHHVNVSMVRAKASGWYVNSILANQEATADG
EEEEECCCCHHCCCCCCCCCEEEEECCCEEEEEEEEEEEEECCCEEEEHHHCCCCCCCCC
YDEALLLDVDGYVSEGSGENFFLVNRGKLYTPDLSSCLDGITRDTIITLAKDAGIEVIEK
CCCEEEEEECCCEECCCCCEEEEEECCEEECCCHHHHHCCCCHHHEEEEECCCCHHHHHH
RITRDEVYTADEAFFTGTAAEVTPIRELDNRTIGSGARGPVTEKLQSAFFDIVSGKNAKY
HHHHHHHEECCCCEEECCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
AHWLTKV
HHHHHCC
>Mature Secondary Structure 
SMADRDGKIWMDGKLIDWRDATIHVLTHTLHYGMGVFEGVRAYKTADGSTAIFRLPEHT
CCCCCCCEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHH
KRLLNSAKIFQMDVPFDQETLEAAQREVVRENKLESCYLRPIIWVGSEKLGVSAKGNTIH
HHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEECEEEEECCCCCCEEECCCEEE
VAIAAWPWGAYLGEDGLAKGIRVKTSSFTRHHVNVSMVRAKASGWYVNSILANQEATADG
EEEEECCCCHHCCCCCCCCCEEEEECCCEEEEEEEEEEEEECCCEEEEHHHCCCCCCCCC
YDEALLLDVDGYVSEGSGENFFLVNRGKLYTPDLSSCLDGITRDTIITLAKDAGIEVIEK
CCCEEEEEECCCEECCCCCEEEEEECCEEECCCHHHHHCCCCHHHEEEEECCCCHHHHHH
RITRDEVYTADEAFFTGTAAEVTPIRELDNRTIGSGARGPVTEKLQSAFFDIVSGKNAKY
HHHHHHHEECCCCEEECCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
AHWLTKV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]