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The map label for this gene is rfaF [H]

Identifier: 161523845

GI number: 161523845

Start: 728441

End: 729478

Strand: Reverse

Name: rfaF [H]

Synonym: Bmul_0665

Alternate gene names: 161523845

Gene position: 729478-728441 (Counterclockwise)

Preceding gene: 161523846

Following gene: 161523844

Centisome position: 21.15

GC content: 67.82

Gene sequence:

>1038_bases
ATGCGTCGAGCGCTGGTTATCGCACCGAACTGGATCGGTGACGCATTGATGGCGCAGCCGCTCTTTGCGCTGCTGAAGAA
ACTCCATCCCCGCATCGCGATCGATGCCGTCGCGCCCACCTGGGTCGCGCCGGTACTCGAACGGATGCCCGAGATCCACG
ATGTCTACGCGACCGATCTCGCGCACGGCAAGCTGCAGCTGCTGCGCCGCTGGCAGCTCGCGAGCGATCTGCGCGACGTC
GGCTACGACGCCGCCTACGTGCTGCCGAATTCGCTGAAATCCGCGGTGATTCCGTGGCTCGCGAACATTCCGCTGCGCAT
CGGCTACACGGGCGAGCACCGCTACGGGCTGTTGAACGTGCGGCACGCGAACCCCGACAAGTCCGCCGAACGGCCGCCGA
TGACGACGCACTATGCGGCGCTCGCGTACGCGCCGGGTGCGAAGCTGCCCGAGTCGATGAAGACGCTGCCGCCGCCGCGC
CTCGACGCGGACCTGAACGAGACGGCGCGCGTCTCCGCGCGCTTCAATCTCGATACGCGCAAGCCGCTCGTCGTGTTCTG
CCCGGGTGCGGAATACGGGCCGGCCAAGCGCTGGCCGCCCGAGCATTTCGCGACGCTCGCGACGATCGTCCACCAGTCGT
TCCCGTATACGCAGATCGTCGCGCTCGGCTCGCAGAAGGACGCGGCCGCCGCGCAGGCGATCGCCGATCACGCGCCGAAC
GTGCGCAATCTCTGCGGGCAGACCTCGCTGTCCGAGGCGTGCGCGCTGATCGCACGCGCGAACGCGGTCGTCACGAACGA
TTCGGGGCTGATGCACGTCGCCGCCGCGCTGCGCCGGCCGCTCGTCGCGCTATACGGGTCGACCGATCCGCGCCACACTC
CCCCGCTGTCGGACCTGGCGAAGGTACAATGGCTGCATCTCGAATGCAGTCCCTGTTTCGAGCGCGAGTGCCCGCTCGGC
CACCTGAATTGTCTGCGCGAACTCGGCCCCGAACAGGTATTCGGCGATTTGCGCGGCATGCTCGTCGGGCAGCGCTGA

Upstream 100 bases:

>100_bases
CGCAGCCGGGCTTGTTCCGGCACGCCGCCGTTTCCCTATCTGGCAACCCGAACGCGGCGCTCGCGCGGCGCGTTTCATTA
CGACATCGGAAATCGACCTG

Downstream 100 bases:

>100_bases
CCTTGACCGGCGGCGTCGCACGGCGCGCCGGGCAATCCGATTCACCGGTGCGCGGCGGCCCCACCCGGGCCGCCGTGCTG
AATACGGCGCGCGACGCGCG

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MRRALVIAPNWIGDALMAQPLFALLKKLHPRIAIDAVAPTWVAPVLERMPEIHDVYATDLAHGKLQLLRRWQLASDLRDV
GYDAAYVLPNSLKSAVIPWLANIPLRIGYTGEHRYGLLNVRHANPDKSAERPPMTTHYAALAYAPGAKLPESMKTLPPPR
LDADLNETARVSARFNLDTRKPLVVFCPGAEYGPAKRWPPEHFATLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPN
VRNLCGQTSLSEACALIARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLAKVQWLHLECSPCFERECPLG
HLNCLRELGPEQVFGDLRGMLVGQR

Sequences:

>Translated_345_residues
MRRALVIAPNWIGDALMAQPLFALLKKLHPRIAIDAVAPTWVAPVLERMPEIHDVYATDLAHGKLQLLRRWQLASDLRDV
GYDAAYVLPNSLKSAVIPWLANIPLRIGYTGEHRYGLLNVRHANPDKSAERPPMTTHYAALAYAPGAKLPESMKTLPPPR
LDADLNETARVSARFNLDTRKPLVVFCPGAEYGPAKRWPPEHFATLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPN
VRNLCGQTSLSEACALIARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLAKVQWLHLECSPCFERECPLG
HLNCLRELGPEQVFGDLRGMLVGQR
>Mature_345_residues
MRRALVIAPNWIGDALMAQPLFALLKKLHPRIAIDAVAPTWVAPVLERMPEIHDVYATDLAHGKLQLLRRWQLASDLRDV
GYDAAYVLPNSLKSAVIPWLANIPLRIGYTGEHRYGLLNVRHANPDKSAERPPMTTHYAALAYAPGAKLPESMKTLPPPR
LDADLNETARVSARFNLDTRKPLVVFCPGAEYGPAKRWPPEHFATLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPN
VRNLCGQTSLSEACALIARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLAKVQWLHLECSPCFERECPLG
HLNCLRELGPEQVFGDLRGMLVGQR

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=353, Percent_Identity=42.7762039660057, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 37894; Mature: 37894

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRALVIAPNWIGDALMAQPLFALLKKLHPRIAIDAVAPTWVAPVLERMPEIHDVYATDL
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHH
AHGKLQLLRRWQLASDLRDVGYDAAYVLPNSLKSAVIPWLANIPLRIGYTGEHRYGLLNV
HHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEE
RHANPDKSAERPPMTTHYAALAYAPGAKLPESMKTLPPPRLDADLNETARVSARFNLDTR
EECCCCCCCCCCCCCHHHHHEEECCCCCCCHHHHCCCCCCCCCCCCCHHEEEEEECCCCC
KPLVVFCPGAEYGPAKRWPPEHFATLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPN
CCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCC
VRNLCGQTSLSEACALIARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLA
HHHHHCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
KVQWLHLECSPCFERECPLGHLNCLRELGPEQVFGDLRGMLVGQR
HEEEEEEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRRALVIAPNWIGDALMAQPLFALLKKLHPRIAIDAVAPTWVAPVLERMPEIHDVYATDL
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHH
AHGKLQLLRRWQLASDLRDVGYDAAYVLPNSLKSAVIPWLANIPLRIGYTGEHRYGLLNV
HHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEE
RHANPDKSAERPPMTTHYAALAYAPGAKLPESMKTLPPPRLDADLNETARVSARFNLDTR
EECCCCCCCCCCCCCHHHHHEEECCCCCCCHHHHCCCCCCCCCCCCCHHEEEEEECCCCC
KPLVVFCPGAEYGPAKRWPPEHFATLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPN
CCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCC
VRNLCGQTSLSEACALIARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLA
HHHHHCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
KVQWLHLECSPCFERECPLGHLNCLRELGPEQVFGDLRGMLVGQR
HEEEEEEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]