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The map label for this gene is proP [H]

Identifier: 161523827

GI number: 161523827

Start: 709769

End: 711427

Strand: Reverse

Name: proP [H]

Synonym: Bmul_0647

Alternate gene names: 161523827

Gene position: 711427-709769 (Counterclockwise)

Preceding gene: 161523828

Following gene: 161523826

Centisome position: 20.63

GC content: 65.1

Gene sequence:

>1659_bases
ATGGCAACGTTAGGCGGGCAAATTGCACACTCGCCGATGACGGCCGAAGAGAAGAAGGTGATCTTCGCGTCGTCGCTCGG
TACGGTGTTCGAGTGGTACGACTTCTACCTGGCCGGGTCGCTCGCGGCCTACATCAGCAAGAGCTTCTTCTCCGGCGTCA
ATCCGACCGCGGCCTTCATCTTCACGCTGCTCGGCTTCGCGGCCGGCTTCGCGGTGCGGCCGTTCGGCGCACTCGTGTTC
GGCCGGCTCGGCGACCTCGTCGGCCGCAAGCACACGTTCCTCGTGACGATCGTGATCATGGGCGTCTCGACGTTCGTGGT
CGGCTTCCTGCCCGGCTACGCGTCGATCGGCATCGCCGCGCCGGTGATCTTCATCGCGATGCGGCTGCTGCAGGGCCTCG
CGCTCGGCGGCGAGTACGGCGGTGCGGCGACCTACGTCGCCGAGCATGCGCCGGCGAATCGCCGCGGCTTCTACACCGCG
TGGATCCAGACGACCGCGACGCTCGGCCTGTTCCTGTCGCTGCTCGTGATCCTCGGCGTGCGCACGTTCATCGGTGAAGA
GGCGTTCGGCGCATGGGGCTGGCGCGTGCCGTTCGTCGCGTCGATCCTGCTGCTCGCGGTGTCGGTGTGGATCCGGATGC
AGCTGAACGAATCGCCGGTGTTCCTGCGCATCAAGTCGGAAGGCAAGACGTCGAAGGCGCCGCTGACGGAAGCGTTCGGC
CAGTGGAAGAACCTGAAGATCGTGATTCTCGCGCTCGTCGGCCTGACGGCCGGCCAGGCCGTCGTGTGGTACACGGGCCA
GTTCTACGCGCTGTTCTTCCTGACGCAGACGCTGAAGGTCGACGGGGCCAGCGCGAACATCCTGATCGCGATCGCGCTGC
TGATCGGCACGCCGTTCTTCCTGTTCTTCGGTTCGCTGTCGGACAAGATCGGTCGCAAGCCGATCATCCTCGCGGGCTGC
CTGATCGCCGCGCTCACCTACTTCCCGCTGTTCAAGGCGCTCACGCACTACGCGAACCCGCAGCTCGAGATCGCGACGCA
AAAGGCGCCGATCTCGGTCGTCGCCGATCCGGCAACGTGCTCGTTCCAGTTCAACCCGGTCGGCACGTCGAAGTTCACGA
ACTCGTGCGACATCGCGAAGAGCGCGCTTGCGAAGGCCGGCCTGAACTACGAGAACGTCGCGGCACCGGCCGGCACGACC
GCGCAGATCAAGGTGGGCGACACGGTGATCCCGGCCTATGACGGCAAGGCCGCCGATGCGAAGGCGCAGGGTGCGGCGTT
CGACAAGACGCTCGCGTCGACGCTGAAGGCGGCCGGCTATCCGGCGAAGGCCGATCCGGCGCAGCTGAACTGGCCGATGA
CGATCGTGATCCTGACGATCCTCGTGATCTACGTGACGATGGTCTACGGGCCGATCGCGGCGATGCTGGTCGAGATGTTT
CCGACGCGGATCCGCTACACGTCGATGTCGCTGCCCTATCACATCGGCAACGGCTGGTTCGGCGGGTTCCTGCCGGCGAC
CGCGTTCGCGATCGTCGCGGCGAAGGGCGACATCTATTCGGGGCTGTGGTATCCGATCGTGATCGCGCTGGTTACGTTCG
TGATCGGGCTGCTGTTCGTCAAGGAGACGAAGGACTCGAACATCTACGCGCAGGATTGA

Upstream 100 bases:

>100_bases
CGCCAGTCAGTGCGCCGTAAGTTTCCGTGCCAATAATCGTCGACAGGCCCGCCCATCCAAGGCGGCCGCACATCTATATC
AAGAGACTTGGAGACGATTC

Downstream 100 bases:

>100_bases
CGGATAGGCACGGGGACGGCGCCGTGCCGGCGGATCGGCCGGCGCCCCATTTGCCCGCCTGCGCACCCGTGGTGCGGCTG
CTCGGCGACTTTTTTCGCGC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MATLGGQIAHSPMTAEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGALVF
GRLGDLVGRKHTFLVTIVIMGVSTFVVGFLPGYASIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTA
WIQTTATLGLFLSLLVILGVRTFIGEEAFGAWGWRVPFVASILLLAVSVWIRMQLNESPVFLRIKSEGKTSKAPLTEAFG
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFGSLSDKIGRKPIILAGC
LIAALTYFPLFKALTHYANPQLEIATQKAPISVVADPATCSFQFNPVGTSKFTNSCDIAKSALAKAGLNYENVAAPAGTT
AQIKVGDTVIPAYDGKAADAKAQGAAFDKTLASTLKAAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIVIALVTFVIGLLFVKETKDSNIYAQD

Sequences:

>Translated_552_residues
MATLGGQIAHSPMTAEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGALVF
GRLGDLVGRKHTFLVTIVIMGVSTFVVGFLPGYASIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTA
WIQTTATLGLFLSLLVILGVRTFIGEEAFGAWGWRVPFVASILLLAVSVWIRMQLNESPVFLRIKSEGKTSKAPLTEAFG
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFGSLSDKIGRKPIILAGC
LIAALTYFPLFKALTHYANPQLEIATQKAPISVVADPATCSFQFNPVGTSKFTNSCDIAKSALAKAGLNYENVAAPAGTT
AQIKVGDTVIPAYDGKAADAKAQGAAFDKTLASTLKAAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIVIALVTFVIGLLFVKETKDSNIYAQD
>Mature_551_residues
ATLGGQIAHSPMTAEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGALVFG
RLGDLVGRKHTFLVTIVIMGVSTFVVGFLPGYASIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAW
IQTTATLGLFLSLLVILGVRTFIGEEAFGAWGWRVPFVASILLLAVSVWIRMQLNESPVFLRIKSEGKTSKAPLTEAFGQ
WKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFGSLSDKIGRKPIILAGCL
IAALTYFPLFKALTHYANPQLEIATQKAPISVVADPATCSFQFNPVGTSKFTNSCDIAKSALAKAGLNYENVAAPAGTTA
QIKVGDTVIPAYDGKAADAKAQGAAFDKTLASTLKAAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMFP
TRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIVIALVTFVIGLLFVKETKDSNIYAQD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=325, Percent_Identity=35.3846153846154, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1788292, Length=328, Percent_Identity=34.1463414634146, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1788942, Length=337, Percent_Identity=34.7181008902077, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1789941, Length=220, Percent_Identity=42.2727272727273, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082231, Length=226, Percent_Identity=26.5486725663717, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082404, Length=324, Percent_Identity=25, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59017; Mature: 58886

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLGGQIAHSPMTAEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFI
CCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FTLLGFAAGFAVRPFGALVFGRLGDLVGRKHTFLVTIVIMGVSTFVVGFLPGYASIGIAA
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAWIQTTATLGLFLSLLVILGV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
RTFIGEEAFGAWGWRVPFVASILLLAVSVWIRMQLNESPVFLRIKSEGKTSKAPLTEAFG
HHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCCHHHHHC
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFF
CCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCHHH
LFFGSLSDKIGRKPIILAGCLIAALTYFPLFKALTHYANPQLEIATQKAPISVVADPATC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEE
SFQFNPVGTSKFTNSCDIAKSALAKAGLNYENVAAPAGTTAQIKVGDTVIPAYDGKAADA
EEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCEEEECCCCCCCCC
KAQGAAFDKTLASTLKAAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMF
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIVIALVTFVIGLLFV
HHHEEEEEECCCEEECCCCCCHHHHHHHHHHHEECCCHHCCHHHHHHHHHHHHHHHHHHH
KETKDSNIYAQD
HCCCCCCCEECC
>Mature Secondary Structure 
ATLGGQIAHSPMTAEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFI
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FTLLGFAAGFAVRPFGALVFGRLGDLVGRKHTFLVTIVIMGVSTFVVGFLPGYASIGIAA
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAWIQTTATLGLFLSLLVILGV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
RTFIGEEAFGAWGWRVPFVASILLLAVSVWIRMQLNESPVFLRIKSEGKTSKAPLTEAFG
HHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCCHHHHHC
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFF
CCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCHHH
LFFGSLSDKIGRKPIILAGCLIAALTYFPLFKALTHYANPQLEIATQKAPISVVADPATC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEE
SFQFNPVGTSKFTNSCDIAKSALAKAGLNYENVAAPAGTTAQIKVGDTVIPAYDGKAADA
EEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCEEEECCCCCCCCC
KAQGAAFDKTLASTLKAAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMF
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIVIALVTFVIGLLFV
HHHEEEEEECCCEEECCCCCCHHHHHHHHHHHEECCCHHCCHHHHHHHHHHHHHHHHHHH
KETKDSNIYAQD
HCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]