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The map label for this gene is pfpI [H]

Identifier: 161523736

GI number: 161523736

Start: 605783

End: 606364

Strand: Reverse

Name: pfpI [H]

Synonym: Bmul_0556

Alternate gene names: 161523736

Gene position: 606364-605783 (Counterclockwise)

Preceding gene: 161523737

Following gene: 161523733

Centisome position: 17.58

GC content: 68.21

Gene sequence:

>582_bases
ATGGCAGCGAAGAAGATCCTGTTCCTGACCGGCGATTTCGCCGAAGACTACGAAACGATGGTGCCGTTCCAGGCGCTGCA
GGCGGTCGGCCATCACGTCGACGCCGTATGCCCGGGCAAGCGCGCCGGCGACAAGGTCAAGACCGCGATCCACGATTTCG
AGGGCGACCAGACCTACACCGAGAAGCCCGGCCACCAGTTCACGCTGAATGCGACGTTCGACGACGTCGACGCGTCGCGC
TACGACGCGCTCGCGATCGCGGGCGGCCGCGCGCCGGAATACCTGCGGCTCGATCCGAACGTGATCGCGCTCGTGCGCGC
CTTCGCCGAAGCCGGCAAGCCGATCGCGGCGATCTGTCACGCCGCGCAACTGTTGGCGGCCGCCGACGTGATTCGCGGCA
AGCGCATCTCGGCCTATCCGGCCTGCGCGCCCGAGGTCAAGCTCGCGGGCGGCGAATATGCGGACATTCCGGTCGATGCG
GCCGTCACCGACGCGCCGTTCGTCACCGCACCCGCATGGCCCGCGCATCCGGCCTGGCTGTCGCAGTTCCTCGCGCTGCT
GGGCACGCGCATCGAGCTGTAA

Upstream 100 bases:

>100_bases
TCACGCGCGCGTCGAGCTGCCGGAGTACACTGCGCTTTCGCCCTCGCGGCCGCTTGCGGCCGCGCGAGCGCGCCTCGACG
TTTCTTCAATGGAGAAAGCC

Downstream 100 bases:

>100_bases
GCGGTCGCGCAGACCGATAGATCCGGTGAAATGAAGCGTCGCCGCGCCACGCGCGCGTTCAGCGCTCCACGACCGTCGAG
CCGACGTCCGCGATTCTCGA

Product: PfpI family intracellular peptidase

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MAAKKILFLTGDFAEDYETMVPFQALQAVGHHVDAVCPGKRAGDKVKTAIHDFEGDQTYTEKPGHQFTLNATFDDVDASR
YDALAIAGGRAPEYLRLDPNVIALVRAFAEAGKPIAAICHAAQLLAAADVIRGKRISAYPACAPEVKLAGGEYADIPVDA
AVTDAPFVTAPAWPAHPAWLSQFLALLGTRIEL

Sequences:

>Translated_193_residues
MAAKKILFLTGDFAEDYETMVPFQALQAVGHHVDAVCPGKRAGDKVKTAIHDFEGDQTYTEKPGHQFTLNATFDDVDASR
YDALAIAGGRAPEYLRLDPNVIALVRAFAEAGKPIAAICHAAQLLAAADVIRGKRISAYPACAPEVKLAGGEYADIPVDA
AVTDAPFVTAPAWPAHPAWLSQFLALLGTRIEL
>Mature_192_residues
AAKKILFLTGDFAEDYETMVPFQALQAVGHHVDAVCPGKRAGDKVKTAIHDFEGDQTYTEKPGHQFTLNATFDDVDASRY
DALAIAGGRAPEYLRLDPNVIALVRAFAEAGKPIAAICHAAQLLAAADVIRGKRISAYPACAPEVKLAGGEYADIPVDAA
VTDAPFVTAPAWPAHPAWLSQFLALLGTRIEL

Specific function: Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Escherichia coli, GI87082219, Length=186, Percent_Identity=35.4838709677419, Blast_Score=101, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 20586; Mature: 20454

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKKILFLTGDFAEDYETMVPFQALQAVGHHVDAVCPGKRAGDKVKTAIHDFEGDQTYT
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCC
EKPGHQFTLNATFDDVDASRYDALAIAGGRAPEYLRLDPNVIALVRAFAEAGKPIAAICH
CCCCCEEEEECCCCCCCCCHHCEEEEECCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHH
AAQLLAAADVIRGKRISAYPACAPEVKLAGGEYADIPVDAAVTDAPFVTAPAWPAHPAWL
HHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCEECCCCEEECCCCCCCHHHH
SQFLALLGTRIEL
HHHHHHHHHHCCC
>Mature Secondary Structure 
AAKKILFLTGDFAEDYETMVPFQALQAVGHHVDAVCPGKRAGDKVKTAIHDFEGDQTYT
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCC
EKPGHQFTLNATFDDVDASRYDALAIAGGRAPEYLRLDPNVIALVRAFAEAGKPIAAICH
CCCCCEEEEECCCCCCCCCHHCEEEEECCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHH
AAQLLAAADVIRGKRISAYPACAPEVKLAGGEYADIPVDAAVTDAPFVTAPAWPAHPAWL
HHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCEECCCCEEECCCCCCCHHHH
SQFLALLGTRIEL
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626329 [H]