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The map label for this gene is rpoH [H]

Identifier: 161523666

GI number: 161523666

Start: 532510

End: 533445

Strand: Reverse

Name: rpoH [H]

Synonym: Bmul_0486

Alternate gene names: 161523666

Gene position: 533445-532510 (Counterclockwise)

Preceding gene: 161523669

Following gene: 161523665

Centisome position: 15.47

GC content: 64.32

Gene sequence:

>936_bases
GTGAGCAACGCCCTGACCCTCCCGAATACCCTGAGCCCGACGCCGGCCAAGGCCGAATCGGCAGGCTCGCTGGCGCTCGC
AACTCATTCGATGTTGCCGGGTCAGCTTGGCAACATCGATGCGTACATCCAGGCCGTCAACCGCATTCCGCTGCTGACCG
CCGAAGAGGAACGCCAGTACGCAACCGAATACCGCGAAAACAACAACCTGGAGGCTGCGCGCCGCCTGGTGTTGTCGCAC
CTGCGCCTGGTCGTGTCGATCGCGCGCAACTATCTCGGCTACGGGCTGCCGCACGGCGACCTGATCCAGGAAGGCAACAT
CGGTCTGATGAAAGCGGTGAAGCGTTTCGATCCGGCGCAAAACGTGCGCCTGGTCTCGTACGCGATTCACTGGATCAAGG
CCGAAATCCACGAGTACATCCTGCGCAACTGGCGGATGGTGAAGGTCGCGACGACGAAGGCGCAGCGCAAGCTGTTCTTC
AACCTGCGCAGCCACAAGAAAGGCATGCAGGCGATGACGCCCGAGGAGATCGACGGTCTCGCGCAGGAACTGAACGTGAA
GCGCGAAGACGTGACCGAGATGGAAACGCGTCTGTCGGGCGGCGACATCGCGCTCGAAGGGCAAGTCGAGGACGGCGAGG
AATCGTACGCGCCGATCGCCTATCTGGCCGATTCGCACAACGAGCCGACCGCCGTGCTGGCCGCGCGCCAGCGCGACATG
CTGCAGACGGACGGCATCGCCCAGGCGCTCGACGCGCTCGACGCGCGCAGCCGCCGCATCATCGAGGCGCGCTGGCTGCA
TGTCGACGACGACGGCTCGGGCGGCTCGACGCTGCACGATCTCGCGGCCGAATTCGGCGTCTCCGCGGAGCGCATCCGCC
AGATCGAAGCGAGCGCAATGAAAAAGATGCGCACGGCACTCGCCGAATACGCATAA

Upstream 100 bases:

>100_bases
GGCCTCTAAGGTATTAGCACTCGGCTCCACGGAGTGCTAAGATTGGCCTCGGAATCTAACCCGAGACCAGGGTTCATCCC
TGATTCCAAAGGAGTTTTTA

Downstream 100 bases:

>100_bases
TCCGACGTCCAGAACGCATCACCGAAAACCGCTGCCAACCGGCAGCGGTTTTTTTTCGTCCATTCGCCGCTTCGGCTTTG
CGCGTGCATTTACCCGTTCT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSNALTLPNTLSPTPAKAESAGSLALATHSMLPGQLGNIDAYIQAVNRIPLLTAEEERQYATEYRENNNLEAARRLVLSH
LRLVVSIARNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQNVRLVSYAIHWIKAEIHEYILRNWRMVKVATTKAQRKLFF
NLRSHKKGMQAMTPEEIDGLAQELNVKREDVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDM
LQTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAMKKMRTALAEYA

Sequences:

>Translated_311_residues
MSNALTLPNTLSPTPAKAESAGSLALATHSMLPGQLGNIDAYIQAVNRIPLLTAEEERQYATEYRENNNLEAARRLVLSH
LRLVVSIARNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQNVRLVSYAIHWIKAEIHEYILRNWRMVKVATTKAQRKLFF
NLRSHKKGMQAMTPEEIDGLAQELNVKREDVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDM
LQTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAMKKMRTALAEYA
>Mature_310_residues
SNALTLPNTLSPTPAKAESAGSLALATHSMLPGQLGNIDAYIQAVNRIPLLTAEEERQYATEYRENNNLEAARRLVLSHL
RLVVSIARNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQNVRLVSYAIHWIKAEIHEYILRNWRMVKVATTKAQRKLFFN
LRSHKKGMQAMTPEEIDGLAQELNVKREDVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDML
QTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAMKKMRTALAEYA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=287, Percent_Identity=51.219512195122, Blast_Score=277, Evalue=7e-76,
Organism=Escherichia coli, GI1789098, Length=271, Percent_Identity=32.4723247232472, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=32.7800829875519, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34543; Mature: 34411

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNALTLPNTLSPTPAKAESAGSLALATHSMLPGQLGNIDAYIQAVNRIPLLTAEEERQY
CCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCHHHHHHHHHCCCCEECHHHHHH
ATEYRENNNLEAARRLVLSHLRLVVSIARNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHCCCCC
NVRLVSYAIHWIKAEIHEYILRNWRMVKVATTKAQRKLFFNLRSHKKGMQAMTPEEIDGL
CHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
AQELNVKREDVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDM
HHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
LQTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAM
HHHCHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
KKMRTALAEYA
HHHHHHHHHCC
>Mature Secondary Structure 
SNALTLPNTLSPTPAKAESAGSLALATHSMLPGQLGNIDAYIQAVNRIPLLTAEEERQY
CCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCHHHHHHHHHCCCCEECHHHHHH
ATEYRENNNLEAARRLVLSHLRLVVSIARNYLGYGLPHGDLIQEGNIGLMKAVKRFDPAQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHCCCCC
NVRLVSYAIHWIKAEIHEYILRNWRMVKVATTKAQRKLFFNLRSHKKGMQAMTPEEIDGL
CHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
AQELNVKREDVTEMETRLSGGDIALEGQVEDGEESYAPIAYLADSHNEPTAVLAARQRDM
HHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
LQTDGIAQALDALDARSRRIIEARWLHVDDDGSGGSTLHDLAAEFGVSAERIRQIEASAM
HHHCHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
KKMRTALAEYA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]