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The map label for this gene is gcvA [H]

Identifier: 161523594

GI number: 161523594

Start: 454529

End: 455575

Strand: Reverse

Name: gcvA [H]

Synonym: Bmul_0414

Alternate gene names: 161523594

Gene position: 455575-454529 (Counterclockwise)

Preceding gene: 161523598

Following gene: 161523590

Centisome position: 13.21

GC content: 67.62

Gene sequence:

>1047_bases
ATGGACCTGCGCCAGCTGCCCGCGCTGAACGCGATTCGCGCGTTCGAAGCCGCTGCCCGGCACGAGAATTTCTCGCGCGC
CGCCGACGAACTGTTCGTCACGCACGGCGCGGTCAGCCATCAGGTGCGCGCGCTCGAAGACGAACTCGGCGTGAAGCTGT
TCACGCGCAACGGCAAGCGGCTGTGCCTGACCGACGCCGGGATGCGCTACGCGCAGCAGGTCCGCACCGCGCTGATGATG
CTCGCCGACGCGACGCGCCAGGTGCGCGCGAGCGACCGCGATCGCCGGCTCGTCGTGTCGATGCTGTCGTCGTTCGCCGC
GCGCTTCGTCACGCCGCGGATCGGCACGTTCATCGAACGCCATCCGGAAATCGACGTCGAGCTGCAGTCGACGAATGCGC
TGACCGACTTCGCACGCGACGACGTCGATCTCGCGATCCGCTTCGGCTTCGGCAGTTATCCGGGGCTGTATGTCGAGCCG
CTGCTCGACGAGGCGTTCTTTCCGGCGTGCGCGCCGACGCTGAACGGCGGCAAGCTGCCGCAGACGCCCGCCGATCTCGT
CCATTACAACCTGCTGCGCTCGGACGACGAACTGTGGCGGCCGTGGTTCGACGCGGCGGGCCTCGATACGCTGACCGAAC
CGAAGCGCGGCATTCTCTATCAGGACTCGTCGAATCTGCTGCAGGCCGCGATCGAGGGTCAGGGCATCGCGCTCGTGCGT
CGCTCGCTCGCGCTGCACGATCTACTCGACGGCCGGCTCGTGCGGCTGTTCGACATCGACGGGCCGAGCCCGTGGCATTA
CTTCTTCGTGTGCCCGCCGCCGCTGCTCGGCACGCCGCGCGTGCAGGCATTCAGGACGTGGATTCTTCAGGAAGTCGCGC
AGTTCAAGCTGCTCTGCGACGAACTCGATGCGCGGCGTGCGGCCGGCAAGTGTCCGCAGGAGTGTGCGCGAGAAGGGGGA
TGGAAGGGGATTGCCGCCCGTTCCGCCGGCACCGGCGGAACGGAGGACGAAACGCACGCGGCAACGGCCGTTACAGCACG
ACCTTGA

Upstream 100 bases:

>100_bases
TCGACTCCGCCAAAAAACGCTTCGATGGCTACAATCGTAGTCATTGCGACACGTCCGACCAAACGACCAATTTTCACGTC
GATGTGAGGAAAATTAGCAA

Downstream 100 bases:

>100_bases
CCATCGGATGGCCGGTCAGCGCGCGGTAGATGTTGTCGAGCTGTTCCTGGCTCGTCGCGCGCACCGTGACGGTCAGCCCC
GTATAGTTGCCGCCGCTCGA

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEDELGVKLFTRNGKRLCLTDAGMRYAQQVRTALMM
LADATRQVRASDRDRRLVVSMLSSFAARFVTPRIGTFIERHPEIDVELQSTNALTDFARDDVDLAIRFGFGSYPGLYVEP
LLDEAFFPACAPTLNGGKLPQTPADLVHYNLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLQAAIEGQGIALVR
RSLALHDLLDGRLVRLFDIDGPSPWHYFFVCPPPLLGTPRVQAFRTWILQEVAQFKLLCDELDARRAAGKCPQECAREGG
WKGIAARSAGTGGTEDETHAATAVTARP

Sequences:

>Translated_348_residues
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEDELGVKLFTRNGKRLCLTDAGMRYAQQVRTALMM
LADATRQVRASDRDRRLVVSMLSSFAARFVTPRIGTFIERHPEIDVELQSTNALTDFARDDVDLAIRFGFGSYPGLYVEP
LLDEAFFPACAPTLNGGKLPQTPADLVHYNLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLQAAIEGQGIALVR
RSLALHDLLDGRLVRLFDIDGPSPWHYFFVCPPPLLGTPRVQAFRTWILQEVAQFKLLCDELDARRAAGKCPQECAREGG
WKGIAARSAGTGGTEDETHAATAVTARP
>Mature_348_residues
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEDELGVKLFTRNGKRLCLTDAGMRYAQQVRTALMM
LADATRQVRASDRDRRLVVSMLSSFAARFVTPRIGTFIERHPEIDVELQSTNALTDFARDDVDLAIRFGFGSYPGLYVEP
LLDEAFFPACAPTLNGGKLPQTPADLVHYNLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLQAAIEGQGIALVR
RSLALHDLLDGRLVRLFDIDGPSPWHYFFVCPPPLLGTPRVQAFRTWILQEVAQFKLLCDELDARRAAGKCPQECAREGG
WKGIAARSAGTGGTEDETHAATAVTARP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=40.7534246575342, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1786448, Length=299, Percent_Identity=32.7759197324415, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=29.2517006802721, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=29.9651567944251, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786401, Length=248, Percent_Identity=29.8387096774194, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=36.875, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789639, Length=252, Percent_Identity=25.7936507936508, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=167, Percent_Identity=25.748502994012, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789440, Length=243, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787128, Length=141, Percent_Identity=30.4964539007092, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788748, Length=168, Percent_Identity=27.3809523809524, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787589, Length=143, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87081978, Length=162, Percent_Identity=28.3950617283951, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 38616; Mature: 38616

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEDELGVKLFTRNGKR
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEECCCCE
LCLTDAGMRYAQQVRTALMMLADATRQVRASDRDRRLVVSMLSSFAARFVTPRIGTFIER
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
HPEIDVELQSTNALTDFARDDVDLAIRFGFGSYPGLYVEPLLDEAFFPACAPTLNGGKLP
CCCCEEEECCCCHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
QTPADLVHYNLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLQAAIEGQGIALVR
CCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCHHHHH
RSLALHDLLDGRLVRLFDIDGPSPWHYFFVCPPPLLGTPRVQAFRTWILQEVAQFKLLCD
HHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELDARRAAGKCPQECAREGGWKGIAARSAGTGGTEDETHAATAVTARP
HHHHHHHCCCCHHHHHHCCCCCCEEECCCCCCCCCCCCHHHEEEECCC
>Mature Secondary Structure
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEDELGVKLFTRNGKR
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEECCCCE
LCLTDAGMRYAQQVRTALMMLADATRQVRASDRDRRLVVSMLSSFAARFVTPRIGTFIER
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
HPEIDVELQSTNALTDFARDDVDLAIRFGFGSYPGLYVEPLLDEAFFPACAPTLNGGKLP
CCCCEEEECCCCHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
QTPADLVHYNLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLQAAIEGQGIALVR
CCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCHHHHH
RSLALHDLLDGRLVRLFDIDGPSPWHYFFVCPPPLLGTPRVQAFRTWILQEVAQFKLLCD
HHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELDARRAAGKCPQECAREGGWKGIAARSAGTGGTEDETHAATAVTARP
HHHHHHHCCCCHHHHHHCCCCCCEEECCCCCCCCCCCCHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]