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The map label for this gene is bioB [H]

Identifier: 161523542

GI number: 161523542

Start: 400141

End: 400380

Strand: Reverse

Name: bioB [H]

Synonym: Bmul_0362

Alternate gene names: 161523542

Gene position: 400380-400141 (Counterclockwise)

Preceding gene: 161523544

Following gene: 161523541

Centisome position: 11.61

GC content: 68.33

Gene sequence:

>240_bases
GTGCGCACGATCGCGGTGGCGCGCATCACCATGCCGAAGGCCGTCGTGCGTCTGTCGGCCGGCCGCGAACAGCTCGACGA
CGGCCTGCAGGCGCTCTGCTTCCTCGCCGGCGCGAATTCCATGTTCTACGGCGACCAGCTGCTGACGACGAGCAACCCGC
AGACGCAGAAGGATCGCGCGCTGTTCGAGCGTCTCGGGATTCGCGCGAGCGAGGCCGATGCGCTGGCGGAACACGCATGA

Upstream 100 bases:

>100_bases
CGCACGAATTCCGTATCCGGAATCGGCGCCGATCAACAATCCGGTCGCGATCGAAGGCACGCCGCTCGCCGGCACCGCGC
CGCTCGATCCGTTCGAATTC

Downstream 100 bases:

>100_bases
GCGTCGCACGCTGCGCCGCATGACGAAAGCCGGGACGTGTCCCGGCTTTTTTCATTGGCGCGGCGGGGCGAAGCACGTGC
ATCGGCGCCGCGTCCGCACT

Product: biotin and thiamin synthesis associated protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 79; Mature: 79

Protein sequence:

>79_residues
MRTIAVARITMPKAVVRLSAGREQLDDGLQALCFLAGANSMFYGDQLLTTSNPQTQKDRALFERLGIRASEADALAEHA

Sequences:

>Translated_79_residues
MRTIAVARITMPKAVVRLSAGREQLDDGLQALCFLAGANSMFYGDQLLTTSNPQTQKDRALFERLGIRASEADALAEHA
>Mature_79_residues
MRTIAVARITMPKAVVRLSAGREQLDDGLQALCFLAGANSMFYGDQLLTTSNPQTQKDRALFERLGIRASEADALAEHA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=76, Percent_Identity=59.2105263157895, Blast_Score=97, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321725, Length=72, Percent_Identity=45.8333333333333, Blast_Score=70, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 8593; Mature: 8593

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIAVARITMPKAVVRLSAGREQLDDGLQALCFLAGANSMFYGDQLLTTSNPQTQKDRA
CCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHH
LFERLGIRASEADALAEHA
HHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure
MRTIAVARITMPKAVVRLSAGREQLDDGLQALCFLAGANSMFYGDQLLTTSNPQTQKDRA
CCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHH
LFERLGIRASEADALAEHA
HHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA