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The map label for this gene is lysC1 [H]

Identifier: 161509504

GI number: 161509504

Start: 1355764

End: 1357146

Strand: Reverse

Name: lysC1 [H]

Synonym: USA300HOU_1271

Alternate gene names: 161509504

Gene position: 1357146-1355764 (Counterclockwise)

Preceding gene: 161509517

Following gene: 161509502

Centisome position: 47.24

GC content: 31.45

Gene sequence:

>1383_bases
ATGAAAGTTTCCAAATTCGGTGGTAGCTCCGTCTCCAATGCTACTCAAATTAAAAAGGTTTTAAATATTGTTAATTCTGA
TCCAGAGCGAAAGATTGTTATCGTTTCTGCTCCAGGTAAAAGACATGATAATGATATTAAAACAACTGATTTGTTAATCA
GATTATATGAAAAGGTCATTAATCATCTTGATTATCATGATAAAAAAAGAGAAATTATTCAGCGTTATGATGATATTGTA
AAAGAATTGCAAATGGATGAAAGTATTTTACGGACGATAGATGTGACTTTGGAACATTATATTAATCAATTAAAAAATGA
ACCAAAGAGACTATTAGATGCATTACTTTCTTGTGGTGAAGATTTTAATGCGCAATTGATAGCTTTATATAATAATAGTC
AAGGTATACCAACAAAATATATATCACCGAAGGAAGCGGGTATTTTTGTTACTGACGTCCCACAAGAAGCACAAATATTA
GATAGTGCTTACTCTGAAATTAAAAAATTAAGAGATTATGATGAAAAACTAATTATCCCAGGATTTTTTGGCGTATCTCA
TGAAGGATATATAGTTACATTTCCACGTGGCGGATCAGATATAACTGGCGCCATCATATCAAGTGGTGTTAGAGCCACAA
TTTATGAAAATTTCACCGATGTGTCTGGTATTTATAAAGCTAATCCAAATATCATCAAAGATCCTGAATTAATCGAAGAA
ATCACTTATCGAGAAATGCGTGAATTATCTTATGCTGGTTTTAGTGTTTTCCATGATGAAGCCTTACAACCACTTTATAA
AGATCGCATCCCTGTGGTGATTAAAAATACGAACCGCCCTCAAGATAAGGGTACATTTATAGTGCATGATAGAGAAATAA
ATGCGAAAAATGTGATTAGCGGTATAAGTTGTGATAAAGATTTTACCGTTATTAATATCAAAAAGTATTTAATGAATAGA
CAAGTCGGCTTTACTAGAAAGATATTAGGTGTCTTAGAGGATAATAATATTTCATTTGATCATATGCCTTCAGGTATTGA
TACTATCAGTATCGTTATGCGTTCAAAACAAATTCAAAATAAAGAAACAAAAGTATTAAATGAAATTCGTCAAAAATGTG
ATGTTGATGAGCTAAGTATTGAGCATGATTTAGCAATATTAATGATTGTCGGCGAAGGTATGAATAAAGTCATCGGTACT
GCAAATAAAATTACACATGCCTTAGCTGAATCAAAAATTAACTTAAAAATGATTAACCAAGGTGCTTCGGAAATCTCGAT
GATGTTTGGAATTCATGTAGATGATGCTGAAAAAGCTGTATTATCTACATATGAGTTTTGCTATCACGGGGTTGCATTAA
AAAACTTGTGTGATAAAAACTAA

Upstream 100 bases:

>100_bases
TTGTAAAGTTCGTAATATTCAAAATATAAATACATAATATCATTCTTACTTACAAAACTGAATACAATTTTCTGATTTTT
TAGAAAGGTTGGCATGCACT

Downstream 100 bases:

>100_bases
TATAAAAGCAATTCGTAACACATGTATTCAACTGCGTTATGAATTGCTTTTTCTTAATTATTTATTGTTCTCTGGATTGC
ATCCTTAATACGTTGCTCAC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIV
KELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPELIEE
ITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNR
QVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGT
ANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN

Sequences:

>Translated_460_residues
MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIV
KELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPELIEE
ITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNR
QVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGT
ANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN
>Mature_460_residues
MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIV
KELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPELIEE
ITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNR
QVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGT
ANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=472, Percent_Identity=26.9067796610169, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1790455, Length=458, Percent_Identity=25.1091703056769, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790376, Length=302, Percent_Identity=25.4966887417219, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320893, Length=488, Percent_Identity=25, Blast_Score=119, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 52004; Mature: 52004

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVI
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
NHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
DFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEKLIIP
CCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEC
GFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPELIEE
CEECCCCCCEEEEECCCCCCHHHHHHHCCCEEEEHHCCCCCCCEEECCCCCCCCHHHHHH
ITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVIS
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCHHHHHC
GISCDKDFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQN
CCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC
KETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINLKMINQ
HHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCCHHHHHCCHHHHHHHHHHCCCCEEEECC
GASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN
CHHHEEHHHEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVI
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
NHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
DFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEKLIIP
CCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEC
GFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPELIEE
CEECCCCCCEEEEECCCCCCHHHHHHHCCCEEEEHHCCCCCCCEEECCCCCCCCHHHHHH
ITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVIS
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCHHHHHC
GISCDKDFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQN
CCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC
KETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINLKMINQ
HHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCCHHHHHCCHHHHHHHHHHCCCCEEEECC
GASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN
CHHHEEHHHEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]