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The map label for this gene is mnhD2 [H]

Identifier: 161509150

GI number: 161509150

Start: 943386

End: 944882

Strand: Reverse

Name: mnhD2 [H]

Synonym: USA300HOU_0909

Alternate gene names: 161509150

Gene position: 944882-943386 (Counterclockwise)

Preceding gene: 161509151

Following gene: 161509149

Centisome position: 32.89

GC content: 33.67

Gene sequence:

>1497_bases
ATGATTGAAAGTAATATGCTTGTGTTAACGCTAGTTATTCCAGTAATTACAGCCATATTACTTGTCTTTATTGGAAAGCG
TCCTATTATAAAGCGTTATGTCGCACTAGGCGGTACATTATTAACCTTGGTCGCGGCAATTATCAATTTAGCAAATGTTG
TTAAACATGGACCAATACGCGTAGAGCTAGGTTCATGGAAAGCACCATACAGTATTGTCTTTGTATTAGACATATTCAGT
GCCCTACTAATAATTACTAGTATTATTATTACAGCTATTGTGATTTTATATTCATACCAAACAATTGGTATTGAACGTGA
AAGATATTATTATTATTTTTCAGTACTATTTATGTTAATAGGTATCATTGGTGCATTCACAACTGGTGACATTTTCAACC
TATTCGTATATTTCGAAGTATTTTTAATGTCATCTTATTTCTTATTAGTAATTGGTTCTACAAAAATTCAACTACAAGAA
ACAATAAAATATGTTCTTGTTAACGTTGTATCGTCATCCTTCTTCGTAATGGGTGTAGCGATTTTATATTCAGTAGTAGG
AACATTGAATCTAGCAGATATAAGCAATAAATTAGCTAACCTTTCAGCGCATGACAGTGGCTTAGTCAATATCGTTTTCA
TACTATTTATCTTTGTTTTTGCTACAAAAGCTGGCGTATTCCCAATGTTTGTTTGGTTACCTAGTGCATACTATGCACCA
CCAATTCCAATCATTGCATTCTTTGGTGCTTTGTTAACAAAAGTTGGTGTTTATGCAATCGCTAGAACATTGAGCCTATT
CTTTAGTGATAATGTAAGCTTTTCACATTATGTCATTTTATTCTTAGCACTATTAACGATCATCTTTGGTTGTGTCGGTG
CTGTTGCATATGCCAACATTAAAAAAATCATCCTATACAATGTCATGATTGCTGTAGGTGTGATCTTAGTCGGCGTTGCA
ATGATGACAGAGTCAGGTATGATTGGGGCTATTTACTATACATTGCACGACATGTTAGTAAAACTTGCATTATTCTTGCT
TATCGGAATTATGATTAAAATTACAGGCACTGCTGATTTAAGACAATTTGGTGGCTTAATTAAACGATATCCTGTACTTG
GTTGGTCATTCTTTATCGCAGCATTAAGTTTAGCTGGCATTCCCCCACTCAGTGGCTTTTATGGAAAATTTTTTATTGTC
CAATCAACATTTGAAAGAGGATTTTATTTAAGTGGTGTCATCGTCTTATTATCAAGTTTAGTCGTATTATATTCTGTCAT
TCGCATATTCCTACAAGGCTTTTTCGGTCAACCAAAAGGATATGATCTTAATAACAAAGTCGACGTCAAATATTTAACTA
CGATTGCAATCGTTGCAGTTGTGATAACTGTTTTATATGGATTGTCAGCAGATTACTTATATCCAATGGTAAAAGCTGGT
GCAGAAACATTCTACAATCCATCAACCTACGTTAAAGCTGTATTAGGAGGTAAATAA

Upstream 100 bases:

>100_bases
ATCGCCTTTGCGACAACAGCCTTTTTCTTAGTATTAGCATTTAGAACATATAAAGAATTAGGCACAGATAACGTTGAGAG
TATGAAAGGAGTTCCAGAAG

Downstream 100 bases:

>100_bases
CATGGCTGTACAATTAGTATTAAACTTTATTATCGCAGTGTTTTGGTTGTTTGTGACAAATAGTTACACAACAAATAACT
TTGTGTTAGGTTTCATCTTT

Product: putative monovalent cation/H+ antiporter subunit D

Products: H2; oxidized ferredoxin [C]

Alternate protein names: Mnh complex subunit D1 [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLVAAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFS
ALLIITSIIITAIVILYSYQTIGIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQE
TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAGVFPMFVWLPSAYYAP
PIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVA
MMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIV
QSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAG
AETFYNPSTYVKAVLGGK

Sequences:

>Translated_498_residues
MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLVAAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFS
ALLIITSIIITAIVILYSYQTIGIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQE
TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAGVFPMFVWLPSAYYAP
PIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVA
MMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIV
QSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAG
AETFYNPSTYVKAVLGGK
>Mature_498_residues
MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLVAAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFS
ALLIITSIIITAIVILYSYQTIGIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQE
TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAGVFPMFVWLPSAYYAP
PIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVA
MMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIV
QSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAG
AETFYNPSTYVKAVLGGK

Specific function: Mnh complex is a Na(+)/H(+) antiporter involved in Na(+) excretion [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=387, Percent_Identity=25.8397932816537, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1788827, Length=391, Percent_Identity=26.8542199488491, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788613, Length=352, Percent_Identity=26.7045454545455, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1788831, Length=394, Percent_Identity=25.1269035532995, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI2367154, Length=331, Percent_Identity=25.3776435045317, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788829, Length=437, Percent_Identity=23.3409610983982, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI145693160, Length=381, Percent_Identity=25.1968503937008, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788614, Length=418, Percent_Identity=22.7272727272727, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 54773; Mature: 54773

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLVAAIINLANVVKHGPIR
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEE
VELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTIGIERERYYYYFSVLFMLI
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQETIKYVLVNVVSSSFFVMGVA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAGVFPMFVWLPSAYYAP
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
PIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIILYNVMIAVGVILVGVAMMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIVQSTFERGFYLSGVIVLLSSL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEHHHHCCHHHHHHHHHHHHH
VVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
AETFYNPSTYVKAVLGGK
HHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLVAAIINLANVVKHGPIR
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEE
VELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTIGIERERYYYYFSVLFMLI
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQETIKYVLVNVVSSSFFVMGVA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAGVFPMFVWLPSAYYAP
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
PIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIILYNVMIAVGVILVGVAMMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIVQSTFERGFYLSGVIVLLSSL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEHHHHCCHHHHHHHHHHHHH
VVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
AETFYNPSTYVKAVLGGK
HHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA